Localization and expression of C1QTNF6 in chicken follicles and its regulatory effect on follicular granulosa cells
C1q/tumor necrosis factor-related protein 6 (C1QTNF6) is a newly discovered adiponectin analog that plays a significant role in female reproduction. However, its expression and function in chickens remain unclear. In this study, the full-length coding sequence (CDS) of chicken C1QTNF6 was cloned fro...
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Elsevier
2025-01-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124011167 |
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| author | Chong Li Qi Li Xing Wu Zihao Zhang Jing Li Ruirui Jiang Guoxi Li Xiaojun Liu Xiangtao Kang Zhuanjian Li Donghua Li Yadong Tian |
| author_facet | Chong Li Qi Li Xing Wu Zihao Zhang Jing Li Ruirui Jiang Guoxi Li Xiaojun Liu Xiangtao Kang Zhuanjian Li Donghua Li Yadong Tian |
| author_sort | Chong Li |
| collection | DOAJ |
| description | C1q/tumor necrosis factor-related protein 6 (C1QTNF6) is a newly discovered adiponectin analog that plays a significant role in female reproduction. However, its expression and function in chickens remain unclear. In this study, the full-length coding sequence (CDS) of chicken C1QTNF6 was cloned from adult chicken ovary. We demonstrated that the putative C1QTNF6 protein shares a highly conserved amino acid sequence with known bird homologs. Using quantitative real-time PCR (qRT-PCR) and fluorescence in situ hybridization (FISH), we observed that C1QTNF6 is widely expressed in adult chicken follicle tissues, predominantly in granulosa cells (GCs). In vitro, stimulation with follicle-stimulating hormone (FSH) and luteinizing hormone (LH) significantly increased the expression of C1QTNF6 in chicken preovulatory granulosa cells (PoGCs) and prehierarchical granulosa cells (PhGCs), respectively. Overexpression and knockdown experiments in granulosa cells revealed that C1QTNF6 affects the proliferation and apoptosis of granulosa cells, reducing the expression of steroid hormone synthesis-related genes (STAR, Cyp11a1, Cyp19a1, and 3B-HSD) and hormones (progesterone and estrogen). In granulosa cells (GCs), co-treatment with C1QTNF6 and AdipoRon (an activator of adiponectin receptors) showed that AdipoRon promotes progesterone secretion, while C1QTNF6 inhibits AdipoR1 (adiponectin receptor 1) expression. There was no synergistic effect between C1QTNF6 and AdipoRon in steroid hormone production. Collectively, these findings suggest that C1QTNF6 may be a candidate gene for regulating follicular development and reproductive performance in chickens. |
| format | Article |
| id | doaj-art-425a88af24a24c06b140bf0d6289631d |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-425a88af24a24c06b140bf0d6289631d2025-01-22T05:40:23ZengElsevierPoultry Science0032-57912025-01-011041104538Localization and expression of C1QTNF6 in chicken follicles and its regulatory effect on follicular granulosa cellsChong Li0Qi Li1Xing Wu2Zihao Zhang3Jing Li4Ruirui Jiang5Guoxi Li6Xiaojun Liu7Xiangtao Kang8Zhuanjian Li9Donghua Li10Yadong Tian11College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, PR China; Henan Key laboratory for innovation and utilization of chicken germplasm resources, Zhengzhou, 450046, PR ChinaDepartment of Animal Science, Henan zhumadian Agricultural School, 463000, Zhumadian, PR China; Corresponding author.College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, PR China; Henan Key laboratory for innovation and utilization of chicken germplasm resources, Zhengzhou, 450046, PR ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, PR China; Henan Key laboratory for innovation and utilization of chicken germplasm resources, Zhengzhou, 450046, PR ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, PR China; Henan Key laboratory for innovation and utilization of chicken germplasm resources, Zhengzhou, 450046, PR ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, PR China; Henan Key laboratory for innovation and utilization of chicken germplasm resources, Zhengzhou, 450046, PR ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, PR China; Henan Key laboratory for innovation and utilization of chicken germplasm resources, Zhengzhou, 450046, PR ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, PR China; Henan Key laboratory for innovation and utilization of chicken germplasm resources, Zhengzhou, 450046, PR ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, PR China; Henan Key laboratory for innovation and utilization of chicken germplasm resources, Zhengzhou, 450046, PR ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, PR China; Henan Key laboratory for innovation and utilization of chicken germplasm resources, Zhengzhou, 450046, PR ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, PR China; Henan Key laboratory for innovation and utilization of chicken germplasm resources, Zhengzhou, 450046, PR ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, PR China; Henan Key laboratory for innovation and utilization of chicken germplasm resources, Zhengzhou, 450046, PR China; Corresponding author.C1q/tumor necrosis factor-related protein 6 (C1QTNF6) is a newly discovered adiponectin analog that plays a significant role in female reproduction. However, its expression and function in chickens remain unclear. In this study, the full-length coding sequence (CDS) of chicken C1QTNF6 was cloned from adult chicken ovary. We demonstrated that the putative C1QTNF6 protein shares a highly conserved amino acid sequence with known bird homologs. Using quantitative real-time PCR (qRT-PCR) and fluorescence in situ hybridization (FISH), we observed that C1QTNF6 is widely expressed in adult chicken follicle tissues, predominantly in granulosa cells (GCs). In vitro, stimulation with follicle-stimulating hormone (FSH) and luteinizing hormone (LH) significantly increased the expression of C1QTNF6 in chicken preovulatory granulosa cells (PoGCs) and prehierarchical granulosa cells (PhGCs), respectively. Overexpression and knockdown experiments in granulosa cells revealed that C1QTNF6 affects the proliferation and apoptosis of granulosa cells, reducing the expression of steroid hormone synthesis-related genes (STAR, Cyp11a1, Cyp19a1, and 3B-HSD) and hormones (progesterone and estrogen). In granulosa cells (GCs), co-treatment with C1QTNF6 and AdipoRon (an activator of adiponectin receptors) showed that AdipoRon promotes progesterone secretion, while C1QTNF6 inhibits AdipoR1 (adiponectin receptor 1) expression. There was no synergistic effect between C1QTNF6 and AdipoRon in steroid hormone production. Collectively, these findings suggest that C1QTNF6 may be a candidate gene for regulating follicular development and reproductive performance in chickens.http://www.sciencedirect.com/science/article/pii/S0032579124011167LayersC1QTNF6Follicles, Granulosa cellsAdipoRon |
| spellingShingle | Chong Li Qi Li Xing Wu Zihao Zhang Jing Li Ruirui Jiang Guoxi Li Xiaojun Liu Xiangtao Kang Zhuanjian Li Donghua Li Yadong Tian Localization and expression of C1QTNF6 in chicken follicles and its regulatory effect on follicular granulosa cells Poultry Science Layers C1QTNF6 Follicles, Granulosa cells AdipoRon |
| title | Localization and expression of C1QTNF6 in chicken follicles and its regulatory effect on follicular granulosa cells |
| title_full | Localization and expression of C1QTNF6 in chicken follicles and its regulatory effect on follicular granulosa cells |
| title_fullStr | Localization and expression of C1QTNF6 in chicken follicles and its regulatory effect on follicular granulosa cells |
| title_full_unstemmed | Localization and expression of C1QTNF6 in chicken follicles and its regulatory effect on follicular granulosa cells |
| title_short | Localization and expression of C1QTNF6 in chicken follicles and its regulatory effect on follicular granulosa cells |
| title_sort | localization and expression of c1qtnf6 in chicken follicles and its regulatory effect on follicular granulosa cells |
| topic | Layers C1QTNF6 Follicles, Granulosa cells AdipoRon |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124011167 |
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