Establishment and Application of a Method for the Determination of Ganoderic Acid A
A method for the quantitative determination of ganoderic acid A was constructed using the principle of indirect competitive enzyme-linked immunosorbent assay (ELISA), and this method was used to determine the ganoderic A contents of Ganoderma lucidum samples in the market. The conjugate of ganoderic...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Food Quality |
| Online Access: | http://dx.doi.org/10.1155/2020/6621853 |
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| author | Shengyun Li Yaowu Yuan Chenchen Yu Hao Gao Jianxin Tan Yiling Tian |
| author_facet | Shengyun Li Yaowu Yuan Chenchen Yu Hao Gao Jianxin Tan Yiling Tian |
| author_sort | Shengyun Li |
| collection | DOAJ |
| description | A method for the quantitative determination of ganoderic acid A was constructed using the principle of indirect competitive enzyme-linked immunosorbent assay (ELISA), and this method was used to determine the ganoderic A contents of Ganoderma lucidum samples in the market. The conjugate of ganoderic acid A and bovine serum albumin was used for four rounds of immunization on test rabbits to obtain rabbit antiganoderic acid A antibody IgG. The enzyme-labeled plate was coated with the conjugate of ganoderic acid A and ovalbumin. The first stage reaction in the indirect competitive ELISA was that the conjugate of ganoderic acid A in the sample competed with the conjugate coated on the enzyme-labeled plate to bind rabbit antibodies. The second stage reaction was the combination of goat anti-rabbit IgG–horseradish peroxidase and rabbit antiganoderic acid A antibody IgG. The results of the determination of ganoderic acid A standard by this method showed that the coefficient of variation of repeated wells in the group was <5%, the detection limit of ganoderic acid A was 0.6 μg/L, and ganoderic acid A had a substantial dose-response relationship in the content range of 0.9–72.9 μg/L (R2 = 0.994). This method was used to measure the ganoderic A content of 12 varieties of G. lucidum in the market and showed the obvious differences in the ganoderic acid A contents of the different varieties. This method is simple, fast, and of great importance to the quality control of Ganoderma products. |
| format | Article |
| id | doaj-art-420728a6c5e84e8bb0bc16117e96613d |
| institution | OA Journals |
| issn | 0146-9428 1745-4557 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
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| series | Journal of Food Quality |
| spelling | doaj-art-420728a6c5e84e8bb0bc16117e96613d2025-08-20T02:37:51ZengWileyJournal of Food Quality0146-94281745-45572020-01-01202010.1155/2020/66218536621853Establishment and Application of a Method for the Determination of Ganoderic Acid AShengyun Li0Yaowu Yuan1Chenchen Yu2Hao Gao3Jianxin Tan4Yiling Tian5College of Food Science and Technology, Hebei Agricultural University, No. 289 Lingyusi Street, Baoding 071001, Hebei, ChinaCollege of Food Science and Technology, Hebei Agricultural University, No. 289 Lingyusi Street, Baoding 071001, Hebei, ChinaCollege of Food Science and Technology, Hebei Agricultural University, No. 289 Lingyusi Street, Baoding 071001, Hebei, ChinaCollege of Food Science and Technology, Hebei Agricultural University, No. 289 Lingyusi Street, Baoding 071001, Hebei, ChinaCollege of Food Science and Technology, Hebei Agricultural University, No. 289 Lingyusi Street, Baoding 071001, Hebei, ChinaCollege of Food Science and Technology, Hebei Agricultural University, No. 289 Lingyusi Street, Baoding 071001, Hebei, ChinaA method for the quantitative determination of ganoderic acid A was constructed using the principle of indirect competitive enzyme-linked immunosorbent assay (ELISA), and this method was used to determine the ganoderic A contents of Ganoderma lucidum samples in the market. The conjugate of ganoderic acid A and bovine serum albumin was used for four rounds of immunization on test rabbits to obtain rabbit antiganoderic acid A antibody IgG. The enzyme-labeled plate was coated with the conjugate of ganoderic acid A and ovalbumin. The first stage reaction in the indirect competitive ELISA was that the conjugate of ganoderic acid A in the sample competed with the conjugate coated on the enzyme-labeled plate to bind rabbit antibodies. The second stage reaction was the combination of goat anti-rabbit IgG–horseradish peroxidase and rabbit antiganoderic acid A antibody IgG. The results of the determination of ganoderic acid A standard by this method showed that the coefficient of variation of repeated wells in the group was <5%, the detection limit of ganoderic acid A was 0.6 μg/L, and ganoderic acid A had a substantial dose-response relationship in the content range of 0.9–72.9 μg/L (R2 = 0.994). This method was used to measure the ganoderic A content of 12 varieties of G. lucidum in the market and showed the obvious differences in the ganoderic acid A contents of the different varieties. This method is simple, fast, and of great importance to the quality control of Ganoderma products.http://dx.doi.org/10.1155/2020/6621853 |
| spellingShingle | Shengyun Li Yaowu Yuan Chenchen Yu Hao Gao Jianxin Tan Yiling Tian Establishment and Application of a Method for the Determination of Ganoderic Acid A Journal of Food Quality |
| title | Establishment and Application of a Method for the Determination of Ganoderic Acid A |
| title_full | Establishment and Application of a Method for the Determination of Ganoderic Acid A |
| title_fullStr | Establishment and Application of a Method for the Determination of Ganoderic Acid A |
| title_full_unstemmed | Establishment and Application of a Method for the Determination of Ganoderic Acid A |
| title_short | Establishment and Application of a Method for the Determination of Ganoderic Acid A |
| title_sort | establishment and application of a method for the determination of ganoderic acid a |
| url | http://dx.doi.org/10.1155/2020/6621853 |
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