Migration and proliferation drive the emergence of patterns in co-cultures of differentiating vascular progenitor cells
Vascular cells self-organize into unique structures guided by cell proliferation, migration, and/or differentiation from neighboring cells, mechanical factors, and/or soluble signals. However, the relative contribution of each of these factors remains unclear. Our objective was to develop a computat...
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| Language: | English |
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AIMS Press
2024-08-01
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| Series: | Mathematical Biosciences and Engineering |
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| Online Access: | https://www.aimspress.com/article/doi/10.3934/mbe.2024295 |
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| author | Jose E. Zamora Alvarado Kara E. McCloskey Ajay Gopinathan |
| author_facet | Jose E. Zamora Alvarado Kara E. McCloskey Ajay Gopinathan |
| author_sort | Jose E. Zamora Alvarado |
| collection | DOAJ |
| description | Vascular cells self-organize into unique structures guided by cell proliferation, migration, and/or differentiation from neighboring cells, mechanical factors, and/or soluble signals. However, the relative contribution of each of these factors remains unclear. Our objective was to develop a computational model to explore the different factors affecting the emerging micropatterns in 2D. This was accomplished by developing a stochastic on-lattice population-based model starting with vascular progenitor cells with the potential to proliferate, migrate, and/or differentiate into either endothelial cells or smooth muscle cells. The simulation results yielded patterns that were qualitatively and quantitatively consistent with experimental observations. Our results suggested that post-differentiation cell migration and proliferation when balanced could generate between 30–70% of each cell type enabling the formation of vascular patterns. Moreover, the cell-to-cell sensing could enhance the robustness of this patterning. These findings computationally supported that 2D patterning is mechanistically similar to current microfluidic platforms that take advantage of the migration-directed self-assembly of mature endothelial and mural cells to generate perfusable 3D vasculature in permissible hydrogel environments and suggest that stem or progenitor cells may not be fully necessary components in many tissue formations like those formed by vasculogenesis. |
| format | Article |
| id | doaj-art-42065763f67646939db4066a109cb7f5 |
| institution | Kabale University |
| issn | 1551-0018 |
| language | English |
| publishDate | 2024-08-01 |
| publisher | AIMS Press |
| record_format | Article |
| series | Mathematical Biosciences and Engineering |
| spelling | doaj-art-42065763f67646939db4066a109cb7f52025-01-23T07:47:47ZengAIMS PressMathematical Biosciences and Engineering1551-00182024-08-012186731675710.3934/mbe.2024295Migration and proliferation drive the emergence of patterns in co-cultures of differentiating vascular progenitor cellsJose E. Zamora Alvarado0Kara E. McCloskey1Ajay Gopinathan2School of Engineering, University of California Merced, Merced, CA 95343, USASchool of Engineering, University of California Merced, Merced, CA 95343, USAGraduate Program in Materials and Biomaterials Science and Engineering, University of California Merced, Merced, CA 95343, USAVascular cells self-organize into unique structures guided by cell proliferation, migration, and/or differentiation from neighboring cells, mechanical factors, and/or soluble signals. However, the relative contribution of each of these factors remains unclear. Our objective was to develop a computational model to explore the different factors affecting the emerging micropatterns in 2D. This was accomplished by developing a stochastic on-lattice population-based model starting with vascular progenitor cells with the potential to proliferate, migrate, and/or differentiate into either endothelial cells or smooth muscle cells. The simulation results yielded patterns that were qualitatively and quantitatively consistent with experimental observations. Our results suggested that post-differentiation cell migration and proliferation when balanced could generate between 30–70% of each cell type enabling the formation of vascular patterns. Moreover, the cell-to-cell sensing could enhance the robustness of this patterning. These findings computationally supported that 2D patterning is mechanistically similar to current microfluidic platforms that take advantage of the migration-directed self-assembly of mature endothelial and mural cells to generate perfusable 3D vasculature in permissible hydrogel environments and suggest that stem or progenitor cells may not be fully necessary components in many tissue formations like those formed by vasculogenesis.https://www.aimspress.com/article/doi/10.3934/mbe.2024295computational modelstem cell differentiationvascular developmentpatterningendothelial cellssmooth muscle cells |
| spellingShingle | Jose E. Zamora Alvarado Kara E. McCloskey Ajay Gopinathan Migration and proliferation drive the emergence of patterns in co-cultures of differentiating vascular progenitor cells Mathematical Biosciences and Engineering computational model stem cell differentiation vascular development patterning endothelial cells smooth muscle cells |
| title | Migration and proliferation drive the emergence of patterns in co-cultures of differentiating vascular progenitor cells |
| title_full | Migration and proliferation drive the emergence of patterns in co-cultures of differentiating vascular progenitor cells |
| title_fullStr | Migration and proliferation drive the emergence of patterns in co-cultures of differentiating vascular progenitor cells |
| title_full_unstemmed | Migration and proliferation drive the emergence of patterns in co-cultures of differentiating vascular progenitor cells |
| title_short | Migration and proliferation drive the emergence of patterns in co-cultures of differentiating vascular progenitor cells |
| title_sort | migration and proliferation drive the emergence of patterns in co cultures of differentiating vascular progenitor cells |
| topic | computational model stem cell differentiation vascular development patterning endothelial cells smooth muscle cells |
| url | https://www.aimspress.com/article/doi/10.3934/mbe.2024295 |
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