LOW TEMPERATURE STORAGE OF ACTINOBACILLUS PLEUROPNEUMONIAE CULTURES
Basic tasks during storage of any microorganism cultures including Actinobacillus pleuropneumoniae involve maintenance of their vitality and stable taxonomically relevant signs. In different collections the microorganism vitality is mainly maintained using the following methods: repeated passages, l...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Da Vinci Media
2018-04-01
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| Series: | Ветеринария сегодня |
| Subjects: | |
| Online Access: | https://veterinary.arriah.ru/jour/article/view/331 |
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| Summary: | Basic tasks during storage of any microorganism cultures including Actinobacillus pleuropneumoniae involve maintenance of their vitality and stable taxonomically relevant signs. In different collections the microorganism vitality is mainly maintained using the following methods: repeated passages, lyophilisation and storage with mineral oil at low and ultra low temperatures. Promising method of long-term storage of microorganisms includes cryopreservation that demonstrates a number of advantages. The paper examines approaches to possible improvement of means for long term storage of actinobacilli. Experimental data are reported that were obtained during research of potential effect of liquid nitrogen freezing on vitality and some biological properties of actinobacilli. After one year storage (observation period) at -196 °С the microorganism cultures were demonstrated to preserve vitality and species-specific cultural-morphologic and biochemical properties. Factors relevant for Actinobacillus pleuropneumoniae preparation for cryopreservation and affecting storage results are demonstrated. |
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| ISSN: | 2304-196X 2658-6959 |