Molecular characterization of the TUBG1 meshwork's influence on Cytoskeletal organization
The γ-tubulin (TUBG) meshwork is a central regulator of cellular architecture, orchestrating processes such as microtubule nucleation, mitochondrial organization, and genomic integrity. This study investigates the molecular impact of TUBG depletion on the cytoskeleton. Knockdown of TUBG using single...
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Elsevier
2025-01-01
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| Series: | Heliyon |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844025002099 |
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| author | Darina Malycheva Maria Alvarado-Kristensson |
| author_facet | Darina Malycheva Maria Alvarado-Kristensson |
| author_sort | Darina Malycheva |
| collection | DOAJ |
| description | The γ-tubulin (TUBG) meshwork is a central regulator of cellular architecture, orchestrating processes such as microtubule nucleation, mitochondrial organization, and genomic integrity. This study investigates the molecular impact of TUBG depletion on the cytoskeleton. Knockdown of TUBG using single guide RNA disrupted microtubule, vimentin, and lamin B networks while simultaneously reinforcing actin filaments structures. These findings suggest that actin reinforcement may act as a compensatory response to the broader disruption of cytoskeletal integrity. Expression of N-terminal (TUBG1-335) or C-terminal (TUBG334-451) fragments of TUBG1 partially restored these networks, with the C-terminal fragment demonstrating greater effectiveness reestablishing microtubule integrity. Both fragments stabilized vimentin filaments and the nuclear envelope, underscoring TUBG's dual structural and regulatory roles across multiple cytoskeletal systems. This study highlights the critical hubbing properties of TUBG in coordinating cytoskeletal integrity and its potential as a therapeutic target in cytoskeleton-related disorders. |
| format | Article |
| id | doaj-art-41cec886c22349a9bb9523dfd2dd80ea |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-41cec886c22349a9bb9523dfd2dd80ea2025-02-02T05:28:19ZengElsevierHeliyon2405-84402025-01-01112e41829Molecular characterization of the TUBG1 meshwork's influence on Cytoskeletal organizationDarina Malycheva0Maria Alvarado-Kristensson1Molecular Pathology, Department of Translational Medicine, Lund University, SE, 21428 Malmö, SwedenCorresponding author. Molecular Pathology Lund University 53 Inga Marie Nilssons Street, Floor 2 SE, 214 28, Malmö, Sweden.; Molecular Pathology, Department of Translational Medicine, Lund University, SE, 21428 Malmö, SwedenThe γ-tubulin (TUBG) meshwork is a central regulator of cellular architecture, orchestrating processes such as microtubule nucleation, mitochondrial organization, and genomic integrity. This study investigates the molecular impact of TUBG depletion on the cytoskeleton. Knockdown of TUBG using single guide RNA disrupted microtubule, vimentin, and lamin B networks while simultaneously reinforcing actin filaments structures. These findings suggest that actin reinforcement may act as a compensatory response to the broader disruption of cytoskeletal integrity. Expression of N-terminal (TUBG1-335) or C-terminal (TUBG334-451) fragments of TUBG1 partially restored these networks, with the C-terminal fragment demonstrating greater effectiveness reestablishing microtubule integrity. Both fragments stabilized vimentin filaments and the nuclear envelope, underscoring TUBG's dual structural and regulatory roles across multiple cytoskeletal systems. This study highlights the critical hubbing properties of TUBG in coordinating cytoskeletal integrity and its potential as a therapeutic target in cytoskeleton-related disorders.http://www.sciencedirect.com/science/article/pii/S2405844025002099 |
| spellingShingle | Darina Malycheva Maria Alvarado-Kristensson Molecular characterization of the TUBG1 meshwork's influence on Cytoskeletal organization Heliyon |
| title | Molecular characterization of the TUBG1 meshwork's influence on Cytoskeletal organization |
| title_full | Molecular characterization of the TUBG1 meshwork's influence on Cytoskeletal organization |
| title_fullStr | Molecular characterization of the TUBG1 meshwork's influence on Cytoskeletal organization |
| title_full_unstemmed | Molecular characterization of the TUBG1 meshwork's influence on Cytoskeletal organization |
| title_short | Molecular characterization of the TUBG1 meshwork's influence on Cytoskeletal organization |
| title_sort | molecular characterization of the tubg1 meshwork s influence on cytoskeletal organization |
| url | http://www.sciencedirect.com/science/article/pii/S2405844025002099 |
| work_keys_str_mv | AT darinamalycheva molecularcharacterizationofthetubg1meshworksinfluenceoncytoskeletalorganization AT mariaalvaradokristensson molecularcharacterizationofthetubg1meshworksinfluenceoncytoskeletalorganization |