Application of serological methods combined with flow cytometry in the detection of immune hemolytic transfusion reaction
[Objective] To explore the effects of different methods on antibody detection through investigating the causes of cross-matching incompatible in a patient with gastric malignant tumor, and to establish flow cytometry protocol for confirming hemolytic transfusion reaction (HTR). [Methods] Antibodies...
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Institute of Blood Transfusion of Chinese Academy of Medical Sciences
2025-01-01
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| Series: | Zhongguo shuxue zazhi |
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| Online Access: | https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.01.020&lang=en |
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| _version_ | 1832585123277570048 |
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| author | LIU Lei ZENG Qunjuan GONG Guoqin XIANG Dong WANG Zhongying |
| author_facet | LIU Lei ZENG Qunjuan GONG Guoqin XIANG Dong WANG Zhongying |
| author_sort | LIU Lei |
| collection | DOAJ |
| description | [Objective] To explore the effects of different methods on antibody detection through investigating the causes of cross-matching incompatible in a patient with gastric malignant tumor, and to establish flow cytometry protocol for confirming hemolytic transfusion reaction (HTR). [Methods] Antibodies in the patient's serum were identified by red blood cells (RBCs) blood grouping, antibody screening and identification, acid elution test and PEG enhancement test. To confirm HTR, patient RBCs, proximal and distal ends RBCs, separated by capillary centrifugation, were tested by direct antiglobulin test (DAT) and Jka antigen single label and double label flow cytometry. [Results] Routine serological technology revealed the presence of anti-C, e (titer:2) and anti-Jka (titer >1) in the patient’s serum. After separation using capillary centrifugation technology, both the proximal and distal DAT and Jka antigen tests were negative. Both DAT and Jka antigen positive red blood cells (0.21%, 6/6 327) were found in the patient's blood samples by flow cytometry. After separation of blood samples by capillary centrifugation, there were significantly more DAT and Jka antigen double-positive RBCs in the distal end (0.43%, 33/7 707) than in the proximal end (0.09%, 15/7 225). Two blood samples were screened from over 100 donor blood samples that are compatible with the patient's cross-matching, and the transfusion effect was favorable. [Conclusion] Serological methods combined with flow cytometry could improve the sensitivity of antibody detection, provide a more accurate basis for the diagnosis of HTRs, and guarantee the safety of blood transfusion. |
| format | Article |
| id | doaj-art-41c39d2870cb4758b172895d11b06f60 |
| institution | Kabale University |
| issn | 1004-549X |
| language | zho |
| publishDate | 2025-01-01 |
| publisher | Institute of Blood Transfusion of Chinese Academy of Medical Sciences |
| record_format | Article |
| series | Zhongguo shuxue zazhi |
| spelling | doaj-art-41c39d2870cb4758b172895d11b06f602025-01-27T01:46:12ZzhoInstitute of Blood Transfusion of Chinese Academy of Medical SciencesZhongguo shuxue zazhi1004-549X2025-01-0138111612110.13303/j.cjbt.issn.1004-549x.2025.01.0201004-549X(2025)1-0116-06Application of serological methods combined with flow cytometry in the detection of immune hemolytic transfusion reactionLIU Lei0ZENG Qunjuan1GONG Guoqin2XIANG Dong3WANG Zhongying4Shanghai Blood Center, Shanghai 200051, ChinaDazhou Central Hospital, Dazhou 635000, ChinaShanghai Blood Center, Shanghai 200051, ChinaShanghai Blood Center, Shanghai 200051, ChinaShanghai Blood Center, Shanghai 200051, China[Objective] To explore the effects of different methods on antibody detection through investigating the causes of cross-matching incompatible in a patient with gastric malignant tumor, and to establish flow cytometry protocol for confirming hemolytic transfusion reaction (HTR). [Methods] Antibodies in the patient's serum were identified by red blood cells (RBCs) blood grouping, antibody screening and identification, acid elution test and PEG enhancement test. To confirm HTR, patient RBCs, proximal and distal ends RBCs, separated by capillary centrifugation, were tested by direct antiglobulin test (DAT) and Jka antigen single label and double label flow cytometry. [Results] Routine serological technology revealed the presence of anti-C, e (titer:2) and anti-Jka (titer >1) in the patient’s serum. After separation using capillary centrifugation technology, both the proximal and distal DAT and Jka antigen tests were negative. Both DAT and Jka antigen positive red blood cells (0.21%, 6/6 327) were found in the patient's blood samples by flow cytometry. After separation of blood samples by capillary centrifugation, there were significantly more DAT and Jka antigen double-positive RBCs in the distal end (0.43%, 33/7 707) than in the proximal end (0.09%, 15/7 225). Two blood samples were screened from over 100 donor blood samples that are compatible with the patient's cross-matching, and the transfusion effect was favorable. [Conclusion] Serological methods combined with flow cytometry could improve the sensitivity of antibody detection, provide a more accurate basis for the diagnosis of HTRs, and guarantee the safety of blood transfusion.https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.01.020&lang=enflow cytometryanti-c, eanti-jkacapillary centrifugation technologyhemolytic transfusion reaction |
| spellingShingle | LIU Lei ZENG Qunjuan GONG Guoqin XIANG Dong WANG Zhongying Application of serological methods combined with flow cytometry in the detection of immune hemolytic transfusion reaction Zhongguo shuxue zazhi flow cytometry anti-c, e anti-jka capillary centrifugation technology hemolytic transfusion reaction |
| title | Application of serological methods combined with flow cytometry in the detection of immune hemolytic transfusion reaction |
| title_full | Application of serological methods combined with flow cytometry in the detection of immune hemolytic transfusion reaction |
| title_fullStr | Application of serological methods combined with flow cytometry in the detection of immune hemolytic transfusion reaction |
| title_full_unstemmed | Application of serological methods combined with flow cytometry in the detection of immune hemolytic transfusion reaction |
| title_short | Application of serological methods combined with flow cytometry in the detection of immune hemolytic transfusion reaction |
| title_sort | application of serological methods combined with flow cytometry in the detection of immune hemolytic transfusion reaction |
| topic | flow cytometry anti-c, e anti-jka capillary centrifugation technology hemolytic transfusion reaction |
| url | https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.01.020&lang=en |
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