Turnour necrosis factor stimulates endothelin-1 gene expression in cultured bovine endothelial cells
We have studied the effect of human recombinant tumour necrosis factor-α (TNF-α) on gene expression and production of endothelin-1 in cultured bovine aortic endothelial cells. TNF-α (10 and 100 ng ml−1) increased in a time dependent manner the preproendothelin-1 mRNA levels in respect to unstimulate...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
1992-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935192000401 |
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| _version_ | 1832567931049869312 |
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| author | Silvia Orisio Marina Morigi Carla Zoja Norberto Perico Giuseppe Remuzzi |
| author_facet | Silvia Orisio Marina Morigi Carla Zoja Norberto Perico Giuseppe Remuzzi |
| author_sort | Silvia Orisio |
| collection | DOAJ |
| description | We have studied the effect of human recombinant tumour necrosis factor-α (TNF-α) on gene expression and production of endothelin-1 in cultured bovine aortic endothelial cells. TNF-α (10 and 100 ng ml−1) increased in a time dependent manner the preproendothelin-1 mRNA levels in respect to unstimulated endothelial cells. TNF-α induced endothelin-1 gene expression was associated with a parallel increase in the release of the corresponding peptide in the culture medium. These findings suggest that the enhanced synthesis and release of endothelin-1 occurring in conditions of increased generation of TNF, may act as a modulatory factor that counteracts the hypotensive effect and the excessive platelet aggregation and adhesion induced by TNF. |
| format | Article |
| id | doaj-art-41b119a67a8943e395dbe27416a5a245 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1992-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-41b119a67a8943e395dbe27416a5a2452025-02-03T01:00:04ZengWileyMediators of Inflammation0962-93511466-18611992-01-011426326610.1155/S0962935192000401Turnour necrosis factor stimulates endothelin-1 gene expression in cultured bovine endothelial cellsSilvia Orisio0Marina Morigi1Carla Zoja2Norberto Perico3Giuseppe Remuzzi4Mario Negri Institute for Pharmacological Research, Via Gavazzeni 11, Ospedali Riuniti di Bergamo, Bergamo 24100, ItalyMario Negri Institute for Pharmacological Research, Via Gavazzeni 11, Ospedali Riuniti di Bergamo, Bergamo 24100, ItalyMario Negri Institute for Pharmacological Research, Via Gavazzeni 11, Ospedali Riuniti di Bergamo, Bergamo 24100, ItalyMario Negri Institute for Pharmacological Research, Via Gavazzeni 11, Ospedali Riuniti di Bergamo, Bergamo 24100, ItalyMario Negri Institute for Pharmacological Research, Via Gavazzeni 11, Ospedali Riuniti di Bergamo, Bergamo 24100, ItalyWe have studied the effect of human recombinant tumour necrosis factor-α (TNF-α) on gene expression and production of endothelin-1 in cultured bovine aortic endothelial cells. TNF-α (10 and 100 ng ml−1) increased in a time dependent manner the preproendothelin-1 mRNA levels in respect to unstimulated endothelial cells. TNF-α induced endothelin-1 gene expression was associated with a parallel increase in the release of the corresponding peptide in the culture medium. These findings suggest that the enhanced synthesis and release of endothelin-1 occurring in conditions of increased generation of TNF, may act as a modulatory factor that counteracts the hypotensive effect and the excessive platelet aggregation and adhesion induced by TNF.http://dx.doi.org/10.1155/S0962935192000401 |
| spellingShingle | Silvia Orisio Marina Morigi Carla Zoja Norberto Perico Giuseppe Remuzzi Turnour necrosis factor stimulates endothelin-1 gene expression in cultured bovine endothelial cells Mediators of Inflammation |
| title | Turnour necrosis factor stimulates endothelin-1 gene expression in cultured bovine endothelial cells |
| title_full | Turnour necrosis factor stimulates endothelin-1 gene expression in cultured bovine endothelial cells |
| title_fullStr | Turnour necrosis factor stimulates endothelin-1 gene expression in cultured bovine endothelial cells |
| title_full_unstemmed | Turnour necrosis factor stimulates endothelin-1 gene expression in cultured bovine endothelial cells |
| title_short | Turnour necrosis factor stimulates endothelin-1 gene expression in cultured bovine endothelial cells |
| title_sort | turnour necrosis factor stimulates endothelin 1 gene expression in cultured bovine endothelial cells |
| url | http://dx.doi.org/10.1155/S0962935192000401 |
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