Cross-sectional study of proteomic differences between moderate and severe psoriasis
Abstract Although an ongoing understanding of psoriasis vulgaris (PV) pathogenesis, little is known about the proteomic differences between moderate and severe psoriasis. In this cross-sectional study, we evaluated the proteomic differences between moderate and severe psoriasis using data-independen...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-01-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-87252-9 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832571782509363200 |
|---|---|
| author | Lingling Wu Chen Cen Bibo Xie Lihua Hu Jia Huang Ningning Shen Qiang Dong |
| author_facet | Lingling Wu Chen Cen Bibo Xie Lihua Hu Jia Huang Ningning Shen Qiang Dong |
| author_sort | Lingling Wu |
| collection | DOAJ |
| description | Abstract Although an ongoing understanding of psoriasis vulgaris (PV) pathogenesis, little is known about the proteomic differences between moderate and severe psoriasis. In this cross-sectional study, we evaluated the proteomic differences between moderate and severe psoriasis using data-independent acquisition mass spectrometry (DIA-MS). 173 differentially expressed proteins (DEPs) were significantly differentially expressed between the two groups. Among them, 85 proteins were upregulated, while 88 were downregulated (FC ≥ ± 1.5, P < 0.05). Eighteen DEPs were mainly enriched in the IL − 17 signalling pathway, Neutrophil extracellular trap formation, Neutrophil degranulation and NF − kappa B signalling pathway, which were associated with psoriasis pathogenesis. Ingenuity pathway Analysis (IPA) identified TNF and TDP53 as the top upstream up-regulators, while Lipopolysaccharide and YAP1 were the top potential down-regulators. The main active pathways were antimicrobial peptides and PTEN signalling, while the inhibitory pathways were the neutrophil extracellular trap pathway, neutrophil degranulation, and IL-8 signalling. 4D-parallel reaction monitoring (4D-PRM) suggested that KRT6A were downregulated in severe psoriasis. Our data identify Eighteen DEPs as biomarkers of disease severity, and are associated with IL − 17 signalling pathway, Neutrophil extracellular trap formation, NF − kappa B signalling pathway, and defence response to the bacterium. Targeting these molecules and measures to manage infection may improve psoriasis’s severity and therapeutic efficacy. |
| format | Article |
| id | doaj-art-419aa4e2fee24d49939d719fcf2f1aef |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-419aa4e2fee24d49939d719fcf2f1aef2025-02-02T12:20:07ZengNature PortfolioScientific Reports2045-23222025-01-0115111310.1038/s41598-025-87252-9Cross-sectional study of proteomic differences between moderate and severe psoriasisLingling Wu0Chen Cen1Bibo Xie2Lihua Hu3Jia Huang4Ningning Shen5Qiang Dong6Department of Dermatology, Dermatology Hospital of Zhejiang ProvinceDepartment of Dermatology, Dermatology Hospital of Zhejiang ProvinceDepartment of Dermatology, Dermatology Hospital of Zhejiang ProvinceDepartment of Dermatology, Dermatology Hospital of Zhejiang ProvinceDepartment of Dermatology, Dermatology Hospital of Zhejiang ProvinceDepartment of Dermatology, Dermatology Hospital of Zhejiang ProvinceDepartment of Dermatology, Dermatology Hospital of Zhejiang ProvinceAbstract Although an ongoing understanding of psoriasis vulgaris (PV) pathogenesis, little is known about the proteomic differences between moderate and severe psoriasis. In this cross-sectional study, we evaluated the proteomic differences between moderate and severe psoriasis using data-independent acquisition mass spectrometry (DIA-MS). 173 differentially expressed proteins (DEPs) were significantly differentially expressed between the two groups. Among them, 85 proteins were upregulated, while 88 were downregulated (FC ≥ ± 1.5, P < 0.05). Eighteen DEPs were mainly enriched in the IL − 17 signalling pathway, Neutrophil extracellular trap formation, Neutrophil degranulation and NF − kappa B signalling pathway, which were associated with psoriasis pathogenesis. Ingenuity pathway Analysis (IPA) identified TNF and TDP53 as the top upstream up-regulators, while Lipopolysaccharide and YAP1 were the top potential down-regulators. The main active pathways were antimicrobial peptides and PTEN signalling, while the inhibitory pathways were the neutrophil extracellular trap pathway, neutrophil degranulation, and IL-8 signalling. 4D-parallel reaction monitoring (4D-PRM) suggested that KRT6A were downregulated in severe psoriasis. Our data identify Eighteen DEPs as biomarkers of disease severity, and are associated with IL − 17 signalling pathway, Neutrophil extracellular trap formation, NF − kappa B signalling pathway, and defence response to the bacterium. Targeting these molecules and measures to manage infection may improve psoriasis’s severity and therapeutic efficacy.https://doi.org/10.1038/s41598-025-87252-9ProteomicsPsoriasis vulgaris4D-parallel reaction monitoringData-independent acquisition mass spectrometryIngenuity pathway analysisBiomarker |
| spellingShingle | Lingling Wu Chen Cen Bibo Xie Lihua Hu Jia Huang Ningning Shen Qiang Dong Cross-sectional study of proteomic differences between moderate and severe psoriasis Scientific Reports Proteomics Psoriasis vulgaris 4D-parallel reaction monitoring Data-independent acquisition mass spectrometry Ingenuity pathway analysis Biomarker |
| title | Cross-sectional study of proteomic differences between moderate and severe psoriasis |
| title_full | Cross-sectional study of proteomic differences between moderate and severe psoriasis |
| title_fullStr | Cross-sectional study of proteomic differences between moderate and severe psoriasis |
| title_full_unstemmed | Cross-sectional study of proteomic differences between moderate and severe psoriasis |
| title_short | Cross-sectional study of proteomic differences between moderate and severe psoriasis |
| title_sort | cross sectional study of proteomic differences between moderate and severe psoriasis |
| topic | Proteomics Psoriasis vulgaris 4D-parallel reaction monitoring Data-independent acquisition mass spectrometry Ingenuity pathway analysis Biomarker |
| url | https://doi.org/10.1038/s41598-025-87252-9 |
| work_keys_str_mv | AT linglingwu crosssectionalstudyofproteomicdifferencesbetweenmoderateandseverepsoriasis AT chencen crosssectionalstudyofproteomicdifferencesbetweenmoderateandseverepsoriasis AT biboxie crosssectionalstudyofproteomicdifferencesbetweenmoderateandseverepsoriasis AT lihuahu crosssectionalstudyofproteomicdifferencesbetweenmoderateandseverepsoriasis AT jiahuang crosssectionalstudyofproteomicdifferencesbetweenmoderateandseverepsoriasis AT ningningshen crosssectionalstudyofproteomicdifferencesbetweenmoderateandseverepsoriasis AT qiangdong crosssectionalstudyofproteomicdifferencesbetweenmoderateandseverepsoriasis |