MicroRNA-129-3p Suppresses Tumor Progression and Chemoradioresistance in Head and Neck Squamous Cell Carcinoma
(1) Background: MicroRNA-129 (miR-129) participates in tumor progression and chemoresistance in various cancer types. In this study, the role of miR-129-3p, the main mature form of miR-129, in tumor progression and chemoradiotherapy resistance in head and neck cancer was evaluated. (2) Methods: RT-P...
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MDPI AG
2025-01-01
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| Series: | Current Oncology |
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| author | Tae Mi Yoon Sun-Ae Kim Eun Kyung Jung Young-Kook Kim Kyung-Hwa Lee Sang Chul Lim |
| author_facet | Tae Mi Yoon Sun-Ae Kim Eun Kyung Jung Young-Kook Kim Kyung-Hwa Lee Sang Chul Lim |
| author_sort | Tae Mi Yoon |
| collection | DOAJ |
| description | (1) Background: MicroRNA-129 (miR-129) participates in tumor progression and chemoresistance in various cancer types. In this study, the role of miR-129-3p, the main mature form of miR-129, in tumor progression and chemoradiotherapy resistance in head and neck cancer was evaluated. (2) Methods: RT-PCR, western blotting, cell proliferation assays, cell apoptosis assays, and cell invasion and migration assays were used. (3) Results: In this study, the miR-129-3p overexpression suppressed the proliferation, invasion, and migration of SNU1041, SCC15, and SCC25 human HNSCC cell lines. Additionally, it induced apoptosis and enhanced radiation-/cisplatin-induced apoptosis of SNU1041, SCC15, and SCC25 cells. Therefore, miR-129-3p could suppress tumor progression and enhance chemoradiosensitivity in human HNSCC. Furthermore, miR-129-3p was downregulated in fresh tumor tissues from patients with HNSCC compared with that in the adjacent normal mucosa. In a nude mouse xenograft model with SNU15 cells, the miR-129-3p overexpression significantly decreased tumor growth, as measured by tumor weight and volume. (4) Conclusions: Our study provides evidence that miR-129-3p suppresses tumor progression and chemoradioresistance in HNSCC. This finding may serve as a basis for developing miR-129-3p-based therapeutic strategies. |
| format | Article |
| id | doaj-art-4192003094f84a6a940412474e6cd54a |
| institution | Kabale University |
| issn | 1198-0052 1718-7729 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Current Oncology |
| spelling | doaj-art-4192003094f84a6a940412474e6cd54a2025-01-24T13:28:30ZengMDPI AGCurrent Oncology1198-00521718-77292025-01-013215410.3390/curroncol32010054MicroRNA-129-3p Suppresses Tumor Progression and Chemoradioresistance in Head and Neck Squamous Cell CarcinomaTae Mi Yoon0Sun-Ae Kim1Eun Kyung Jung2Young-Kook Kim3Kyung-Hwa Lee4Sang Chul Lim5Departments of Otorhinolaryngology-Head and Neck Surgery, Chonnam National University Medical School, Hwasun Hospital, Hwasun 58128, Jeonnam, Republic of KoreaDepartments of Otorhinolaryngology-Head and Neck Surgery, Chonnam National University Medical School, Hwasun Hospital, Hwasun 58128, Jeonnam, Republic of KoreaDepartments of Otorhinolaryngology-Head and Neck Surgery, Chonnam National University Medical School, Hwasun Hospital, Hwasun 58128, Jeonnam, Republic of KoreaDepartments of Biochemistry, Chonnam National University Medical School, Hwasun Hospital, Hwasun 58128, Jeonnam, Republic of KoreaDepartments of Pathology, Chonnam National University Medical School, Hwasun Hospital, Hwasun 58128, Jeonnam, Republic of KoreaDepartments of Otorhinolaryngology-Head and Neck Surgery, Chonnam National University Medical School, Hwasun Hospital, Hwasun 58128, Jeonnam, Republic of Korea(1) Background: MicroRNA-129 (miR-129) participates in tumor progression and chemoresistance in various cancer types. In this study, the role of miR-129-3p, the main mature form of miR-129, in tumor progression and chemoradiotherapy resistance in head and neck cancer was evaluated. (2) Methods: RT-PCR, western blotting, cell proliferation assays, cell apoptosis assays, and cell invasion and migration assays were used. (3) Results: In this study, the miR-129-3p overexpression suppressed the proliferation, invasion, and migration of SNU1041, SCC15, and SCC25 human HNSCC cell lines. Additionally, it induced apoptosis and enhanced radiation-/cisplatin-induced apoptosis of SNU1041, SCC15, and SCC25 cells. Therefore, miR-129-3p could suppress tumor progression and enhance chemoradiosensitivity in human HNSCC. Furthermore, miR-129-3p was downregulated in fresh tumor tissues from patients with HNSCC compared with that in the adjacent normal mucosa. In a nude mouse xenograft model with SNU15 cells, the miR-129-3p overexpression significantly decreased tumor growth, as measured by tumor weight and volume. (4) Conclusions: Our study provides evidence that miR-129-3p suppresses tumor progression and chemoradioresistance in HNSCC. This finding may serve as a basis for developing miR-129-3p-based therapeutic strategies.https://www.mdpi.com/1718-7729/32/1/54miRNAradioresistanceapoptosismolecular targeted therapyhead and neck squamous cell carcinoma |
| spellingShingle | Tae Mi Yoon Sun-Ae Kim Eun Kyung Jung Young-Kook Kim Kyung-Hwa Lee Sang Chul Lim MicroRNA-129-3p Suppresses Tumor Progression and Chemoradioresistance in Head and Neck Squamous Cell Carcinoma Current Oncology miRNA radioresistance apoptosis molecular targeted therapy head and neck squamous cell carcinoma |
| title | MicroRNA-129-3p Suppresses Tumor Progression and Chemoradioresistance in Head and Neck Squamous Cell Carcinoma |
| title_full | MicroRNA-129-3p Suppresses Tumor Progression and Chemoradioresistance in Head and Neck Squamous Cell Carcinoma |
| title_fullStr | MicroRNA-129-3p Suppresses Tumor Progression and Chemoradioresistance in Head and Neck Squamous Cell Carcinoma |
| title_full_unstemmed | MicroRNA-129-3p Suppresses Tumor Progression and Chemoradioresistance in Head and Neck Squamous Cell Carcinoma |
| title_short | MicroRNA-129-3p Suppresses Tumor Progression and Chemoradioresistance in Head and Neck Squamous Cell Carcinoma |
| title_sort | microrna 129 3p suppresses tumor progression and chemoradioresistance in head and neck squamous cell carcinoma |
| topic | miRNA radioresistance apoptosis molecular targeted therapy head and neck squamous cell carcinoma |
| url | https://www.mdpi.com/1718-7729/32/1/54 |
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