Cardioprotective Effect of Nec-1 in Rats Subjected to MI/R: Downregulation of Autophagy-Like Cell Death
Objective. Necrostatin-1 (Nec-1), an inhibitor of necroptosis, has been reported to protect against myocardial ischemia-reperfusion (MI/R) injury. However, the contribution of the potential antinecroptotic effect of Nec-1 on its infarct limitation and cardiac function improvement effects after MI/R...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Cardiovascular Therapeutics |
| Online Access: | http://dx.doi.org/10.1155/2021/9956814 |
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| _version_ | 1832566271563005952 |
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| author | Liang Wang Xuebai Lv Jue Tian Xiaoliang Wang Ye Wu Hui Rong Liu |
| author_facet | Liang Wang Xuebai Lv Jue Tian Xiaoliang Wang Ye Wu Hui Rong Liu |
| author_sort | Liang Wang |
| collection | DOAJ |
| description | Objective. Necrostatin-1 (Nec-1), an inhibitor of necroptosis, has been reported to protect against myocardial ischemia-reperfusion (MI/R) injury. However, the contribution of the potential antinecroptotic effect of Nec-1 on its infarct limitation and cardiac function improvement effects after MI/R has not been investigated. Methods. The present study investigated the effect of Nec-1 on myocardial infarct size, necroptosis, and cardiac functional recovery in rats subjected to myocardial ischemia-reperfusion (MI/R 30 min/12, 24, 48, and 72 h). Results. The study showed that Nec-1 might reduce myocardial cell death and maintain myoarchitectonic integrity, consequently inhibiting the reactive fibrosis process in rats in myocardial ischemia/late reperfusion. Moreover, the administration of Nec-1 (0.6 mg/kg) at the onset of reperfusion significantly reduced the release of creatine kinase and downregulation of autophagy within 24 h after reperfusion, and there was a significantly positive correlation between them. Conclusion. These results suggest that antinecroptosis treatment may improve the clinical outcomes of patients with ischemic heart disease. |
| format | Article |
| id | doaj-art-41815859425343b1850a3ffd808b8cec |
| institution | Kabale University |
| issn | 1755-5914 1755-5922 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cardiovascular Therapeutics |
| spelling | doaj-art-41815859425343b1850a3ffd808b8cec2025-02-03T01:04:33ZengWileyCardiovascular Therapeutics1755-59141755-59222021-01-01202110.1155/2021/99568149956814Cardioprotective Effect of Nec-1 in Rats Subjected to MI/R: Downregulation of Autophagy-Like Cell DeathLiang Wang0Xuebai Lv1Jue Tian2Xiaoliang Wang3Ye Wu4Hui Rong Liu5Department of Cardiology, Peking University International Hospital, Beijing 030001, ChinaThird Medical Center, The General Hospital of the People's Liberation Army, Beijing 102206, ChinaDepartment of Physiology, Shanxi Medical University, Taiyuan 030001, ChinaDepartment of Physiology, Shanxi Medical University, Taiyuan 030001, ChinaDepartment of Physiology, Shanxi Medical University, Taiyuan 030001, ChinaDepartment of Physiology, Shanxi Medical University, Taiyuan 030001, ChinaObjective. Necrostatin-1 (Nec-1), an inhibitor of necroptosis, has been reported to protect against myocardial ischemia-reperfusion (MI/R) injury. However, the contribution of the potential antinecroptotic effect of Nec-1 on its infarct limitation and cardiac function improvement effects after MI/R has not been investigated. Methods. The present study investigated the effect of Nec-1 on myocardial infarct size, necroptosis, and cardiac functional recovery in rats subjected to myocardial ischemia-reperfusion (MI/R 30 min/12, 24, 48, and 72 h). Results. The study showed that Nec-1 might reduce myocardial cell death and maintain myoarchitectonic integrity, consequently inhibiting the reactive fibrosis process in rats in myocardial ischemia/late reperfusion. Moreover, the administration of Nec-1 (0.6 mg/kg) at the onset of reperfusion significantly reduced the release of creatine kinase and downregulation of autophagy within 24 h after reperfusion, and there was a significantly positive correlation between them. Conclusion. These results suggest that antinecroptosis treatment may improve the clinical outcomes of patients with ischemic heart disease.http://dx.doi.org/10.1155/2021/9956814 |
| spellingShingle | Liang Wang Xuebai Lv Jue Tian Xiaoliang Wang Ye Wu Hui Rong Liu Cardioprotective Effect of Nec-1 in Rats Subjected to MI/R: Downregulation of Autophagy-Like Cell Death Cardiovascular Therapeutics |
| title | Cardioprotective Effect of Nec-1 in Rats Subjected to MI/R: Downregulation of Autophagy-Like Cell Death |
| title_full | Cardioprotective Effect of Nec-1 in Rats Subjected to MI/R: Downregulation of Autophagy-Like Cell Death |
| title_fullStr | Cardioprotective Effect of Nec-1 in Rats Subjected to MI/R: Downregulation of Autophagy-Like Cell Death |
| title_full_unstemmed | Cardioprotective Effect of Nec-1 in Rats Subjected to MI/R: Downregulation of Autophagy-Like Cell Death |
| title_short | Cardioprotective Effect of Nec-1 in Rats Subjected to MI/R: Downregulation of Autophagy-Like Cell Death |
| title_sort | cardioprotective effect of nec 1 in rats subjected to mi r downregulation of autophagy like cell death |
| url | http://dx.doi.org/10.1155/2021/9956814 |
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