Ammonia-Assimilating Bacteria Promote Wheat (<i>Triticum aestivum</i>) Growth and Nitrogen Utilization
Nitrogen fertilizers in agriculture often suffer losses. Ammonia-assimilating bacteria can immobilize ammonia and reduce these losses, but they have not been used in agriculture. This study identified an ammonia-assimilating strain, <i>Enterobacter</i> sp. B12, which assimilated ammonia...
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2024-12-01
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| author | Yuqian Gao Qi Zhang Yuannan Chen Yanqing Yang Chenxiao Zhou Jiayang Yu Yanan Li Liyou Qiu |
| author_facet | Yuqian Gao Qi Zhang Yuannan Chen Yanqing Yang Chenxiao Zhou Jiayang Yu Yanan Li Liyou Qiu |
| author_sort | Yuqian Gao |
| collection | DOAJ |
| description | Nitrogen fertilizers in agriculture often suffer losses. Ammonia-assimilating bacteria can immobilize ammonia and reduce these losses, but they have not been used in agriculture. This study identified an ammonia-assimilating strain, <i>Enterobacter</i> sp. B12, which assimilated ammonia via the glutamate dehydrogenase (GDH) pathway at low levels (5 mM) and the glutamine synthetase (GS)-glutamine-2-oxoglutarate aminotransferase (GOGAT) pathway at high levels (10 mM). Inoculating wheat with B12 increased seedling dry weight, nitrogen accumulation, rhizosphere soil nitrogen content, and root enzyme activities, including GDH, superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD), under both conditions. However, root GS, GOGAT enzyme activities, and ammonia assimilation-related gene expressions were lower than the controls. The results suggest that the ammonia-assimilating bacterium promotes wheat growth, nitrogen accumulation, and soil nitrogen immobilization by establishing an ammonia and amino acid exchange with roots and enhancing root antioxidant capacity, making it a potential plant growth-promoting rhizobacteria (PGPR). |
| format | Article |
| id | doaj-art-417eec29539a44e399ac5f92a2dc89e2 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj-art-417eec29539a44e399ac5f92a2dc89e22025-01-24T13:42:24ZengMDPI AGMicroorganisms2076-26072024-12-011314310.3390/microorganisms13010043Ammonia-Assimilating Bacteria Promote Wheat (<i>Triticum aestivum</i>) Growth and Nitrogen UtilizationYuqian Gao0Qi Zhang1Yuannan Chen2Yanqing Yang3Chenxiao Zhou4Jiayang Yu5Yanan Li6Liyou Qiu7Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture and Rural Affairs, College of Life Sciences, Henan Agricultural University, Zhengzhou 450046, ChinaKey Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture and Rural Affairs, College of Life Sciences, Henan Agricultural University, Zhengzhou 450046, ChinaKey Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture and Rural Affairs, College of Life Sciences, Henan Agricultural University, Zhengzhou 450046, ChinaKey Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture and Rural Affairs, College of Life Sciences, Henan Agricultural University, Zhengzhou 450046, ChinaKey Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture and Rural Affairs, College of Life Sciences, Henan Agricultural University, Zhengzhou 450046, ChinaKey Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture and Rural Affairs, College of Life Sciences, Henan Agricultural University, Zhengzhou 450046, ChinaKey Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture and Rural Affairs, College of Life Sciences, Henan Agricultural University, Zhengzhou 450046, ChinaKey Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture and Rural Affairs, College of Life Sciences, Henan Agricultural University, Zhengzhou 450046, ChinaNitrogen fertilizers in agriculture often suffer losses. Ammonia-assimilating bacteria can immobilize ammonia and reduce these losses, but they have not been used in agriculture. This study identified an ammonia-assimilating strain, <i>Enterobacter</i> sp. B12, which assimilated ammonia via the glutamate dehydrogenase (GDH) pathway at low levels (5 mM) and the glutamine synthetase (GS)-glutamine-2-oxoglutarate aminotransferase (GOGAT) pathway at high levels (10 mM). Inoculating wheat with B12 increased seedling dry weight, nitrogen accumulation, rhizosphere soil nitrogen content, and root enzyme activities, including GDH, superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD), under both conditions. However, root GS, GOGAT enzyme activities, and ammonia assimilation-related gene expressions were lower than the controls. The results suggest that the ammonia-assimilating bacterium promotes wheat growth, nitrogen accumulation, and soil nitrogen immobilization by establishing an ammonia and amino acid exchange with roots and enhancing root antioxidant capacity, making it a potential plant growth-promoting rhizobacteria (PGPR).https://www.mdpi.com/2076-2607/13/1/43ammonia-assimilating bacteriumammonium immobilization<i>Enterobacter</i>wheatammonia nitrogen accumulationtotal nitrogen accumulation |
| spellingShingle | Yuqian Gao Qi Zhang Yuannan Chen Yanqing Yang Chenxiao Zhou Jiayang Yu Yanan Li Liyou Qiu Ammonia-Assimilating Bacteria Promote Wheat (<i>Triticum aestivum</i>) Growth and Nitrogen Utilization Microorganisms ammonia-assimilating bacterium ammonium immobilization <i>Enterobacter</i> wheat ammonia nitrogen accumulation total nitrogen accumulation |
| title | Ammonia-Assimilating Bacteria Promote Wheat (<i>Triticum aestivum</i>) Growth and Nitrogen Utilization |
| title_full | Ammonia-Assimilating Bacteria Promote Wheat (<i>Triticum aestivum</i>) Growth and Nitrogen Utilization |
| title_fullStr | Ammonia-Assimilating Bacteria Promote Wheat (<i>Triticum aestivum</i>) Growth and Nitrogen Utilization |
| title_full_unstemmed | Ammonia-Assimilating Bacteria Promote Wheat (<i>Triticum aestivum</i>) Growth and Nitrogen Utilization |
| title_short | Ammonia-Assimilating Bacteria Promote Wheat (<i>Triticum aestivum</i>) Growth and Nitrogen Utilization |
| title_sort | ammonia assimilating bacteria promote wheat i triticum aestivum i growth and nitrogen utilization |
| topic | ammonia-assimilating bacterium ammonium immobilization <i>Enterobacter</i> wheat ammonia nitrogen accumulation total nitrogen accumulation |
| url | https://www.mdpi.com/2076-2607/13/1/43 |
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