Validation of an UHPLC-MS/MS Method for Screening of Antimicrobial Residues in Eggs and Their Application to Analyses of Eggs from Laying Hens Subjected to Pharmacological Treatment
A multiresidue method by UHPLC/MS-MS was optimized and validated for the screening and semiquantitative detection of antimicrobials residues from tetracyclines, aminoglycosides, quinolones, lincosamides, β-lactams, sulfonamides, and macrolides families in eggs. A qualitative approach was used to ens...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2017/3259073 |
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| author | Letícia Gomes Magnago Caldeira Flávio Alves Santos Andréa Melo Garcia de Oliveira Josefa Abucater Lima Leonardo Francisco de Souza Guilherme Resende da Silva Débora Cristina Sampaio de Assis Silvana de Vasconcelos Cançado |
| author_facet | Letícia Gomes Magnago Caldeira Flávio Alves Santos Andréa Melo Garcia de Oliveira Josefa Abucater Lima Leonardo Francisco de Souza Guilherme Resende da Silva Débora Cristina Sampaio de Assis Silvana de Vasconcelos Cançado |
| author_sort | Letícia Gomes Magnago Caldeira |
| collection | DOAJ |
| description | A multiresidue method by UHPLC/MS-MS was optimized and validated for the screening and semiquantitative detection of antimicrobials residues from tetracyclines, aminoglycosides, quinolones, lincosamides, β-lactams, sulfonamides, and macrolides families in eggs. A qualitative approach was used to ensure adequate sensitivity to detect residues at the level of interest, defined as maximum residue limit (MRL), or less. The applicability of the methods was assessed by analyzing egg samples from hens that had been subjected to pharmacological treatment with neomycin, enrofloxacin, lincomycin, oxytetracycline, and doxycycline during five days and after discontinuation of medication (10 days). The method was adequate for screening all studied analytes in eggs, since the performance parameters ensured a false-compliant rate below or equal to 5%, except for flumequine. In the analyses of eggs from laying hens subjected to pharmacological treatment, all antimicrobial residues were detected throughout the experimental period, even after discontinuation of medication, except for neomycin, demonstrating the applicability of the method for analyses of antimicrobial residues in eggs. |
| format | Article |
| id | doaj-art-4157b536240848ba9a579b988e4541d0 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-4157b536240848ba9a579b988e4541d02025-02-03T00:59:58ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732017-01-01201710.1155/2017/32590733259073Validation of an UHPLC-MS/MS Method for Screening of Antimicrobial Residues in Eggs and Their Application to Analyses of Eggs from Laying Hens Subjected to Pharmacological TreatmentLetícia Gomes Magnago Caldeira0Flávio Alves Santos1Andréa Melo Garcia de Oliveira2Josefa Abucater Lima3Leonardo Francisco de Souza4Guilherme Resende da Silva5Débora Cristina Sampaio de Assis6Silvana de Vasconcelos Cançado7Escola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Av. Antônio Carlos 6627, 30.123-970 Belo Horizonte, MG, BrazilLaboratório Nacional Agropecuário (Lanagro-MG), Av. Rômulo Joviano s/n, 33.600-000 Pedro Leopoldo, MG, BrazilLaboratório Nacional Agropecuário (Lanagro-MG), Av. Rômulo Joviano s/n, 33.600-000 Pedro Leopoldo, MG, BrazilLaboratório Nacional Agropecuário (Lanagro-MG), Av. Rômulo Joviano s/n, 33.600-000 Pedro Leopoldo, MG, BrazilLaboratório Nacional Agropecuário (Lanagro-MG), Av. Rômulo Joviano s/n, 33.600-000 Pedro Leopoldo, MG, BrazilEscola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Av. Antônio Carlos 6627, 30.123-970 Belo Horizonte, MG, BrazilEscola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Av. Antônio Carlos 6627, 30.123-970 Belo Horizonte, MG, BrazilEscola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Av. Antônio Carlos 6627, 30.123-970 Belo Horizonte, MG, BrazilA multiresidue method by UHPLC/MS-MS was optimized and validated for the screening and semiquantitative detection of antimicrobials residues from tetracyclines, aminoglycosides, quinolones, lincosamides, β-lactams, sulfonamides, and macrolides families in eggs. A qualitative approach was used to ensure adequate sensitivity to detect residues at the level of interest, defined as maximum residue limit (MRL), or less. The applicability of the methods was assessed by analyzing egg samples from hens that had been subjected to pharmacological treatment with neomycin, enrofloxacin, lincomycin, oxytetracycline, and doxycycline during five days and after discontinuation of medication (10 days). The method was adequate for screening all studied analytes in eggs, since the performance parameters ensured a false-compliant rate below or equal to 5%, except for flumequine. In the analyses of eggs from laying hens subjected to pharmacological treatment, all antimicrobial residues were detected throughout the experimental period, even after discontinuation of medication, except for neomycin, demonstrating the applicability of the method for analyses of antimicrobial residues in eggs.http://dx.doi.org/10.1155/2017/3259073 |
| spellingShingle | Letícia Gomes Magnago Caldeira Flávio Alves Santos Andréa Melo Garcia de Oliveira Josefa Abucater Lima Leonardo Francisco de Souza Guilherme Resende da Silva Débora Cristina Sampaio de Assis Silvana de Vasconcelos Cançado Validation of an UHPLC-MS/MS Method for Screening of Antimicrobial Residues in Eggs and Their Application to Analyses of Eggs from Laying Hens Subjected to Pharmacological Treatment Journal of Analytical Methods in Chemistry |
| title | Validation of an UHPLC-MS/MS Method for Screening of Antimicrobial Residues in Eggs and Their Application to Analyses of Eggs from Laying Hens Subjected to Pharmacological Treatment |
| title_full | Validation of an UHPLC-MS/MS Method for Screening of Antimicrobial Residues in Eggs and Their Application to Analyses of Eggs from Laying Hens Subjected to Pharmacological Treatment |
| title_fullStr | Validation of an UHPLC-MS/MS Method for Screening of Antimicrobial Residues in Eggs and Their Application to Analyses of Eggs from Laying Hens Subjected to Pharmacological Treatment |
| title_full_unstemmed | Validation of an UHPLC-MS/MS Method for Screening of Antimicrobial Residues in Eggs and Their Application to Analyses of Eggs from Laying Hens Subjected to Pharmacological Treatment |
| title_short | Validation of an UHPLC-MS/MS Method for Screening of Antimicrobial Residues in Eggs and Their Application to Analyses of Eggs from Laying Hens Subjected to Pharmacological Treatment |
| title_sort | validation of an uhplc ms ms method for screening of antimicrobial residues in eggs and their application to analyses of eggs from laying hens subjected to pharmacological treatment |
| url | http://dx.doi.org/10.1155/2017/3259073 |
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