Transcriptional repression of GTL1 under water‐deficit stress promotes anthocyanin biosynthesis to enhance drought tolerance
Abstract The transcription factor GT2‐LIKE 1 (GTL1) has been implicated in orchestrating a transcriptional network of diverse physiological, biochemical, and developmental processes. In response to water‐limiting conditions, GTL1 is a negative regulator of stomatal development, but its potential rol...
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| Format: | Article |
| Language: | English |
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Wiley
2024-05-01
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| Series: | Plant Direct |
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| Online Access: | https://doi.org/10.1002/pld3.594 |
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| _version_ | 1832541398189998080 |
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| author | Noel Anthony Mano Mearaj A. Shaikh Joshua R. Widhalm Chan Yul Yoo Michael V. Mickelbart |
| author_facet | Noel Anthony Mano Mearaj A. Shaikh Joshua R. Widhalm Chan Yul Yoo Michael V. Mickelbart |
| author_sort | Noel Anthony Mano |
| collection | DOAJ |
| description | Abstract The transcription factor GT2‐LIKE 1 (GTL1) has been implicated in orchestrating a transcriptional network of diverse physiological, biochemical, and developmental processes. In response to water‐limiting conditions, GTL1 is a negative regulator of stomatal development, but its potential rolein other water‐deficit responses is unknown. We hypothesized that GTL1 regulates transcriptome changes associated with drought tolerance over leaf developmental stages. To test the hypothesis, gene expression was profiled by RNA‐seq analysis in emerging and expanding leaves of wild‐type and a drought‐tolerant gtl1‐4 knockout mutant under well‐watered and water‐deficit conditions. Our comparative analysis of genotype‐treatment combinations within leaf developmental age identified 459 and 1073 differentially expressed genes in emerging and expanding leaves, respectively, as water‐deficit responsive GTL1‐regulated genes. Transcriptional profiling identified a potential role of GTL1 in two important pathways previously linked to drought tolerance: flavonoid and polyamine biosynthesis. In expanding leaves, negative regulation of GTL1 under water‐deficit conditions promotes biosynthesis of flavonoids and anthocyanins that may contribute to drought tolerance. Quantification of polyamines did not support a role for GTL1 in these drought‐responsive pathways, but this is likely due to the complex nature of polyamine synthesis and turnover. Our global transcriptome analysis suggests that transcriptional repression of GTL1 by water deficit allows plants to activate diverse pathways that collectively contribute to drought tolerance. |
| format | Article |
| id | doaj-art-41000782aaef422abb6dc4ecb83d8f3c |
| institution | Kabale University |
| issn | 2475-4455 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plant Direct |
| spelling | doaj-art-41000782aaef422abb6dc4ecb83d8f3c2025-02-04T08:30:58ZengWileyPlant Direct2475-44552024-05-0185n/an/a10.1002/pld3.594Transcriptional repression of GTL1 under water‐deficit stress promotes anthocyanin biosynthesis to enhance drought toleranceNoel Anthony Mano0Mearaj A. Shaikh1Joshua R. Widhalm2Chan Yul Yoo3Michael V. Mickelbart4Department of Botany and Plant Pathology Purdue University West Lafayette Indiana USACenter for Plant Biology Purdue University West Lafayette Indiana USACenter for Plant Biology Purdue University West Lafayette Indiana USASchool of Biological Sciences The University of Utah Salt Lake City Utah USADepartment of Botany and Plant Pathology Purdue University West Lafayette Indiana USAAbstract The transcription factor GT2‐LIKE 1 (GTL1) has been implicated in orchestrating a transcriptional network of diverse physiological, biochemical, and developmental processes. In response to water‐limiting conditions, GTL1 is a negative regulator of stomatal development, but its potential rolein other water‐deficit responses is unknown. We hypothesized that GTL1 regulates transcriptome changes associated with drought tolerance over leaf developmental stages. To test the hypothesis, gene expression was profiled by RNA‐seq analysis in emerging and expanding leaves of wild‐type and a drought‐tolerant gtl1‐4 knockout mutant under well‐watered and water‐deficit conditions. Our comparative analysis of genotype‐treatment combinations within leaf developmental age identified 459 and 1073 differentially expressed genes in emerging and expanding leaves, respectively, as water‐deficit responsive GTL1‐regulated genes. Transcriptional profiling identified a potential role of GTL1 in two important pathways previously linked to drought tolerance: flavonoid and polyamine biosynthesis. In expanding leaves, negative regulation of GTL1 under water‐deficit conditions promotes biosynthesis of flavonoids and anthocyanins that may contribute to drought tolerance. Quantification of polyamines did not support a role for GTL1 in these drought‐responsive pathways, but this is likely due to the complex nature of polyamine synthesis and turnover. Our global transcriptome analysis suggests that transcriptional repression of GTL1 by water deficit allows plants to activate diverse pathways that collectively contribute to drought tolerance.https://doi.org/10.1002/pld3.594leaf developmentpolyamineRNA‐seqtranscription factor |
| spellingShingle | Noel Anthony Mano Mearaj A. Shaikh Joshua R. Widhalm Chan Yul Yoo Michael V. Mickelbart Transcriptional repression of GTL1 under water‐deficit stress promotes anthocyanin biosynthesis to enhance drought tolerance Plant Direct leaf development polyamine RNA‐seq transcription factor |
| title | Transcriptional repression of GTL1 under water‐deficit stress promotes anthocyanin biosynthesis to enhance drought tolerance |
| title_full | Transcriptional repression of GTL1 under water‐deficit stress promotes anthocyanin biosynthesis to enhance drought tolerance |
| title_fullStr | Transcriptional repression of GTL1 under water‐deficit stress promotes anthocyanin biosynthesis to enhance drought tolerance |
| title_full_unstemmed | Transcriptional repression of GTL1 under water‐deficit stress promotes anthocyanin biosynthesis to enhance drought tolerance |
| title_short | Transcriptional repression of GTL1 under water‐deficit stress promotes anthocyanin biosynthesis to enhance drought tolerance |
| title_sort | transcriptional repression of gtl1 under water deficit stress promotes anthocyanin biosynthesis to enhance drought tolerance |
| topic | leaf development polyamine RNA‐seq transcription factor |
| url | https://doi.org/10.1002/pld3.594 |
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