Bacterial Quality and Molecular Detection of Food Poisoning Virulence Genes Isolated from Nasser Lake Fish, Aswan, Egypt
The microbial analysis of fish is critical for ensuring overall health. Uncooked fish can serve as a conduit for transmitting several types of microbes; the current investigation sought to assess the bacterial levels in various kinds of fish from Nasser Lake, Aswan, Egypt, considered the chief sourc...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | International Journal of Food Science |
| Online Access: | http://dx.doi.org/10.1155/2024/6095430 |
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| author | Nady Khairy Elbarbary Mohamed K. Dandrawy Ghada Hadad Maha Abdelhaseib Amna A. A. Osman Rawaf Alenazy Ibrahim Elbagory Neveen M. Abdelmotilib Fagelnour Elnoamany Ghada A. Ibrahim Reda A. Gomaa |
| author_facet | Nady Khairy Elbarbary Mohamed K. Dandrawy Ghada Hadad Maha Abdelhaseib Amna A. A. Osman Rawaf Alenazy Ibrahim Elbagory Neveen M. Abdelmotilib Fagelnour Elnoamany Ghada A. Ibrahim Reda A. Gomaa |
| author_sort | Nady Khairy Elbarbary |
| collection | DOAJ |
| description | The microbial analysis of fish is critical for ensuring overall health. Uncooked fish can serve as a conduit for transmitting several types of microbes; the current investigation sought to assess the bacterial levels in various kinds of fish from Nasser Lake, Aswan, Egypt, considered the chief source of potable water in Egypt. Two hundred and fifty fish samples, including 50 of each Oreochromis niloticus, Sander lucioperca, Lates niloticus, Clarias gariepinus, and Mormyrus kannume, from Nasser Lake, Aswan, Egypt, were collected to detect the bacterial load, isolation, and identification of Aeromonas hydrophila, Pseudomonas aeruginosa, and Vibrio parahaemolyticus and their virulence genes. The findings revealed that Oreochromis niloticus and Clarias gariepinus exhibited higher bacterial loads than other fish species. Incidences of bacterial contamination among examined fishes were 28.8%, 20.4%, and 16% for Aeromonas hydrophila, Pseudomonas aeruginosa, and Vibrio parahaemolyticus, respectively. Additionally, PCR analysis detected the presence of aerA (60%) and Act (40%) genes in A. hydrophila, rpoB (70%) and LasB (30%) genes in P. aeruginosa, and ToxR (70%) and tdh (50%) genes in V. parahaemolyticus. The study suggested that the bacterial contamination levels in Oreochromis niloticus and Clarias gariepinus could be notably more significant than in other species that could potentially be harmful to the consumers, especially considering the identification of particular bacteria known to cause foodborne illnesses. Further recommendations emphasized that regular monitoring and assessments are required to preserve their quality. |
| format | Article |
| id | doaj-art-40e73e730c3b45008baa6846c882b0f4 |
| institution | Kabale University |
| issn | 2314-5765 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Food Science |
| spelling | doaj-art-40e73e730c3b45008baa6846c882b0f42025-02-03T07:23:38ZengWileyInternational Journal of Food Science2314-57652024-01-01202410.1155/2024/6095430Bacterial Quality and Molecular Detection of Food Poisoning Virulence Genes Isolated from Nasser Lake Fish, Aswan, EgyptNady Khairy Elbarbary0Mohamed K. Dandrawy1Ghada Hadad2Maha Abdelhaseib3Amna A. A. Osman4Rawaf Alenazy5Ibrahim Elbagory6Neveen M. Abdelmotilib7Fagelnour Elnoamany8Ghada A. Ibrahim9Reda A. Gomaa10Department of Food Hygiene and ControlDepartment of Food Hygiene and ControlDepartment of Animal Hygiene and ZoonosesDepartment of Food HygieneDepartment of Food Science and TechnologyDepartment of Medical LaboratoryDepartment of PharmaceuticsDepartment of Food TechnologyGeneral Administration for Laboratories AffairsDepartment of BacteriologyDepartment of Food Science and TechnologyThe microbial analysis of fish is critical for ensuring overall health. Uncooked fish can serve as a conduit for transmitting several types of microbes; the current investigation sought to assess the bacterial levels in various kinds of fish from Nasser Lake, Aswan, Egypt, considered the chief source of potable water in Egypt. Two hundred and fifty fish samples, including 50 of each Oreochromis niloticus, Sander lucioperca, Lates niloticus, Clarias gariepinus, and Mormyrus kannume, from Nasser Lake, Aswan, Egypt, were collected to detect the bacterial load, isolation, and identification of Aeromonas hydrophila, Pseudomonas aeruginosa, and Vibrio parahaemolyticus and their virulence genes. The findings revealed that Oreochromis niloticus and Clarias gariepinus exhibited higher bacterial loads than other fish species. Incidences of bacterial contamination among examined fishes were 28.8%, 20.4%, and 16% for Aeromonas hydrophila, Pseudomonas aeruginosa, and Vibrio parahaemolyticus, respectively. Additionally, PCR analysis detected the presence of aerA (60%) and Act (40%) genes in A. hydrophila, rpoB (70%) and LasB (30%) genes in P. aeruginosa, and ToxR (70%) and tdh (50%) genes in V. parahaemolyticus. The study suggested that the bacterial contamination levels in Oreochromis niloticus and Clarias gariepinus could be notably more significant than in other species that could potentially be harmful to the consumers, especially considering the identification of particular bacteria known to cause foodborne illnesses. Further recommendations emphasized that regular monitoring and assessments are required to preserve their quality.http://dx.doi.org/10.1155/2024/6095430 |
| spellingShingle | Nady Khairy Elbarbary Mohamed K. Dandrawy Ghada Hadad Maha Abdelhaseib Amna A. A. Osman Rawaf Alenazy Ibrahim Elbagory Neveen M. Abdelmotilib Fagelnour Elnoamany Ghada A. Ibrahim Reda A. Gomaa Bacterial Quality and Molecular Detection of Food Poisoning Virulence Genes Isolated from Nasser Lake Fish, Aswan, Egypt International Journal of Food Science |
| title | Bacterial Quality and Molecular Detection of Food Poisoning Virulence Genes Isolated from Nasser Lake Fish, Aswan, Egypt |
| title_full | Bacterial Quality and Molecular Detection of Food Poisoning Virulence Genes Isolated from Nasser Lake Fish, Aswan, Egypt |
| title_fullStr | Bacterial Quality and Molecular Detection of Food Poisoning Virulence Genes Isolated from Nasser Lake Fish, Aswan, Egypt |
| title_full_unstemmed | Bacterial Quality and Molecular Detection of Food Poisoning Virulence Genes Isolated from Nasser Lake Fish, Aswan, Egypt |
| title_short | Bacterial Quality and Molecular Detection of Food Poisoning Virulence Genes Isolated from Nasser Lake Fish, Aswan, Egypt |
| title_sort | bacterial quality and molecular detection of food poisoning virulence genes isolated from nasser lake fish aswan egypt |
| url | http://dx.doi.org/10.1155/2024/6095430 |
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