In Vitro Acid Resistance of Pathogenic Candida Species in Simulated Gastric Fluid
Background and Aims: Although species in the fungal genus Candida are often commensal residents of the gastrointestinal (GI) tract, they can also cause high-mortality systemic candidiasis. Most pathogenic Candida species are dimorphic fungi that exist predominantly in filamentous forms in the invadi...
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Elsevier
2025-01-01
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| Series: | Gastro Hep Advances |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772572324001870 |
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| author | Eri Ikeda Masaya Yamaguchi Masayuki Ono Shigetada Kawabata |
| author_facet | Eri Ikeda Masaya Yamaguchi Masayuki Ono Shigetada Kawabata |
| author_sort | Eri Ikeda |
| collection | DOAJ |
| description | Background and Aims: Although species in the fungal genus Candida are often commensal residents of the gastrointestinal (GI) tract, they can also cause high-mortality systemic candidiasis. Most pathogenic Candida species are dimorphic fungi that exist predominantly in filamentous forms in the invading tissues. Candida albicans is the most prominent pathogen among Candida species, but nonalbicans Candida species have also emerged as important pathogens. The stomach is the most acidic niche in the GI tract and is maintained at pH 1–2 in healthy individuals. The aim of the present study was to determine whether Candida species can survive in gastric fluid and to observe their morphology under varied pH conditions. Methods: We investigated the in vitro survival of the pathogenic Candida species C. albicans, Candida glabrata, Candida parapsilosis, and Candida tropicalis in simulated gastric fluid. Results: We first described that a portion of the 4 Candida species can survive under highly acidic conditions. Moreover, dimorphic Candida species, namely, C. albicans, C. parapsilosis, and C. tropicalis, exhibited yeast–hyphal transition in simulated gastric fluid with elevated pH. Pathogenic filamentous cells had lower acid resistance than yeast cells. Conclusion: These findings may illuminate the migration to the lower GI tract by commensal fungi of the oral cavity. |
| format | Article |
| id | doaj-art-40b5f66aaaff4ef6be7dc7911a3321a6 |
| institution | Kabale University |
| issn | 2772-5723 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Gastro Hep Advances |
| spelling | doaj-art-40b5f66aaaff4ef6be7dc7911a3321a62025-02-06T05:13:04ZengElsevierGastro Hep Advances2772-57232025-01-0144100591In Vitro Acid Resistance of Pathogenic Candida Species in Simulated Gastric FluidEri Ikeda0Masaya Yamaguchi1Masayuki Ono2Shigetada Kawabata3Department of Microbiology, Graduates School of Dentistry, Osaka University, Osaka, Japan; Correspondence: Address correspondence to: Eri Ikeda, DDS, PhD, Assistant Professor, Department of Microbiology, Graduate School of Dental Sciences, Osaka University, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan.Department of Microbiology, Graduates School of Dentistry, Osaka University, Osaka, Japan; Center for Infectious Disease Education and Research (CiDER), Osaka University, Osaka, Japan; Bioinformatics Research Unit, Graduates School of Dentistry, Osaka University, Osaka, Japan; Bioinformatics Center, Research Institute for Microbial Diseases, Osaka University, Osaka, JapanDepartment of Microbiology, Graduates School of Dentistry, Osaka University, Osaka, Japan; Bioinformatics Research Unit, Graduates School of Dentistry, Osaka University, Osaka, JapanDepartment of Microbiology, Graduates School of Dentistry, Osaka University, Osaka, Japan; Center for Infectious Disease Education and Research (CiDER), Osaka University, Osaka, Japan; Shigetada Kawabata, DDS, PhD, Professor, Department of Microbiology, Graduate School of Dental Sciences, Osaka University, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan.Background and Aims: Although species in the fungal genus Candida are often commensal residents of the gastrointestinal (GI) tract, they can also cause high-mortality systemic candidiasis. Most pathogenic Candida species are dimorphic fungi that exist predominantly in filamentous forms in the invading tissues. Candida albicans is the most prominent pathogen among Candida species, but nonalbicans Candida species have also emerged as important pathogens. The stomach is the most acidic niche in the GI tract and is maintained at pH 1–2 in healthy individuals. The aim of the present study was to determine whether Candida species can survive in gastric fluid and to observe their morphology under varied pH conditions. Methods: We investigated the in vitro survival of the pathogenic Candida species C. albicans, Candida glabrata, Candida parapsilosis, and Candida tropicalis in simulated gastric fluid. Results: We first described that a portion of the 4 Candida species can survive under highly acidic conditions. Moreover, dimorphic Candida species, namely, C. albicans, C. parapsilosis, and C. tropicalis, exhibited yeast–hyphal transition in simulated gastric fluid with elevated pH. Pathogenic filamentous cells had lower acid resistance than yeast cells. Conclusion: These findings may illuminate the migration to the lower GI tract by commensal fungi of the oral cavity.http://www.sciencedirect.com/science/article/pii/S2772572324001870Artificial Gastric JuiceCandida AlbicansCandida GlabrataCandida ParapsilosisCandida Tropicalis |
| spellingShingle | Eri Ikeda Masaya Yamaguchi Masayuki Ono Shigetada Kawabata In Vitro Acid Resistance of Pathogenic Candida Species in Simulated Gastric Fluid Gastro Hep Advances Artificial Gastric Juice Candida Albicans Candida Glabrata Candida Parapsilosis Candida Tropicalis |
| title | In Vitro Acid Resistance of Pathogenic Candida Species in Simulated Gastric Fluid |
| title_full | In Vitro Acid Resistance of Pathogenic Candida Species in Simulated Gastric Fluid |
| title_fullStr | In Vitro Acid Resistance of Pathogenic Candida Species in Simulated Gastric Fluid |
| title_full_unstemmed | In Vitro Acid Resistance of Pathogenic Candida Species in Simulated Gastric Fluid |
| title_short | In Vitro Acid Resistance of Pathogenic Candida Species in Simulated Gastric Fluid |
| title_sort | in vitro acid resistance of pathogenic candida species in simulated gastric fluid |
| topic | Artificial Gastric Juice Candida Albicans Candida Glabrata Candida Parapsilosis Candida Tropicalis |
| url | http://www.sciencedirect.com/science/article/pii/S2772572324001870 |
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