Neutrophil-to-lymphocyte ratio is a prognostic factor reflecting immune condition of tumor microenvironment in squamous cell lung cancer

Abstract Inflammatory factors in the peripheral blood, such as the C-reactive protein level and neutrophil-to-lymphocyte ratio (NLR), are prognostic markers in multiple types of cancer, including non-small cell lung cancer (NSCLC). However, the association between inflammatory factors and prognosis...

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Main Authors: Kana Ohashi, Yukari Nishito, Hironori Fukuda, Ryoichi Sadahiro, Yukihiro Yoshida, Shun-ichi Watanabe, Noriko Motoi, Yukiko Sonobe, Hideaki Mizuno, Hiroyuki Tsunoda, Koichiro Tatsumi, Takuji Suzuki, Atsushi Ochiai, Kazunori Aoki
Format: Article
Language:English
Published: Nature Portfolio 2024-01-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-023-50378-9
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author Kana Ohashi
Yukari Nishito
Hironori Fukuda
Ryoichi Sadahiro
Yukihiro Yoshida
Shun-ichi Watanabe
Noriko Motoi
Yukiko Sonobe
Hideaki Mizuno
Hiroyuki Tsunoda
Koichiro Tatsumi
Takuji Suzuki
Atsushi Ochiai
Kazunori Aoki
author_facet Kana Ohashi
Yukari Nishito
Hironori Fukuda
Ryoichi Sadahiro
Yukihiro Yoshida
Shun-ichi Watanabe
Noriko Motoi
Yukiko Sonobe
Hideaki Mizuno
Hiroyuki Tsunoda
Koichiro Tatsumi
Takuji Suzuki
Atsushi Ochiai
Kazunori Aoki
author_sort Kana Ohashi
collection DOAJ
description Abstract Inflammatory factors in the peripheral blood, such as the C-reactive protein level and neutrophil-to-lymphocyte ratio (NLR), are prognostic markers in multiple types of cancer, including non-small cell lung cancer (NSCLC). However, the association between inflammatory factors and prognosis based on histological types has not been adequately reported. In addition, the relationship between these factors and the immune condition of the tumor microenvironment (TME) is unclear. In this single center, retrospective study, we first investigated the relationship between preoperative inflammatory markers and clinical outcomes in 176 patients with NSCLC who underwent surgery. Lung adenocarcinoma (LUAD) showed no significant prognostic marker, whereas for lung squamous cell carcinoma (LUSC), a multivariate analysis showed that a high NLR was significantly associated with postoperative recurrence. In LUSC patients, the median time of postoperative recurrence-free survival in patients with a low NLR was longer than that in patients with a high NLR. We then compared the tumor-infiltrating lymphocyte (TIL) profile with inflammatory markers in peripheral blood and found that the NLR was negatively correlated with the frequencies of T cells and B cells in LUSC tissues. Thus, the NLR is a useful predictive biomarker for postoperative recurrence and may reflect the immune condition of the TME in LUSC.
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spelling doaj-art-4096f7b13c4641aa876bf2b5a00a8b842025-08-20T02:37:57ZengNature PortfolioScientific Reports2045-23222024-01-0114111010.1038/s41598-023-50378-9Neutrophil-to-lymphocyte ratio is a prognostic factor reflecting immune condition of tumor microenvironment in squamous cell lung cancerKana Ohashi0Yukari Nishito1Hironori Fukuda2Ryoichi Sadahiro3Yukihiro Yoshida4Shun-ichi Watanabe5Noriko Motoi6Yukiko Sonobe7Hideaki Mizuno8Hiroyuki Tsunoda9Koichiro Tatsumi10Takuji Suzuki11Atsushi Ochiai12Kazunori Aoki13Department of Immune Medicine, National Cancer Center Research InstituteKamakura Research Laboratories, Chugai Pharmaceutical Co., LtdDepartment of Immune Medicine, National Cancer Center Research InstituteDepartment of Immune Medicine, National Cancer Center Research InstituteDepartment of Thoracic Surgery, National Cancer Center HospitalDepartment of Thoracic Surgery, National Cancer Center HospitalDepartment of Diagnostic Pathology, National Cancer Center HospitalKamakura Research Laboratories, Chugai Pharmaceutical Co., LtdKamakura Research Laboratories, Chugai Pharmaceutical Co., LtdKamakura Research Laboratories, Chugai Pharmaceutical Co., LtdDepartment of Respirology, Graduate School of Medicine, Chiba UniversityDepartment of Respirology, Graduate School of Medicine, Chiba UniversityExploratory Oncology Research and Clinical Trial Center, National Cancer CenterDepartment of Immune Medicine, National Cancer Center Research InstituteAbstract Inflammatory factors in the peripheral blood, such as the C-reactive protein level and neutrophil-to-lymphocyte ratio (NLR), are prognostic markers in multiple types of cancer, including non-small cell lung cancer (NSCLC). However, the association between inflammatory factors and prognosis based on histological types has not been adequately reported. In addition, the relationship between these factors and the immune condition of the tumor microenvironment (TME) is unclear. In this single center, retrospective study, we first investigated the relationship between preoperative inflammatory markers and clinical outcomes in 176 patients with NSCLC who underwent surgery. Lung adenocarcinoma (LUAD) showed no significant prognostic marker, whereas for lung squamous cell carcinoma (LUSC), a multivariate analysis showed that a high NLR was significantly associated with postoperative recurrence. In LUSC patients, the median time of postoperative recurrence-free survival in patients with a low NLR was longer than that in patients with a high NLR. We then compared the tumor-infiltrating lymphocyte (TIL) profile with inflammatory markers in peripheral blood and found that the NLR was negatively correlated with the frequencies of T cells and B cells in LUSC tissues. Thus, the NLR is a useful predictive biomarker for postoperative recurrence and may reflect the immune condition of the TME in LUSC.https://doi.org/10.1038/s41598-023-50378-9
spellingShingle Kana Ohashi
Yukari Nishito
Hironori Fukuda
Ryoichi Sadahiro
Yukihiro Yoshida
Shun-ichi Watanabe
Noriko Motoi
Yukiko Sonobe
Hideaki Mizuno
Hiroyuki Tsunoda
Koichiro Tatsumi
Takuji Suzuki
Atsushi Ochiai
Kazunori Aoki
Neutrophil-to-lymphocyte ratio is a prognostic factor reflecting immune condition of tumor microenvironment in squamous cell lung cancer
Scientific Reports
title Neutrophil-to-lymphocyte ratio is a prognostic factor reflecting immune condition of tumor microenvironment in squamous cell lung cancer
title_full Neutrophil-to-lymphocyte ratio is a prognostic factor reflecting immune condition of tumor microenvironment in squamous cell lung cancer
title_fullStr Neutrophil-to-lymphocyte ratio is a prognostic factor reflecting immune condition of tumor microenvironment in squamous cell lung cancer
title_full_unstemmed Neutrophil-to-lymphocyte ratio is a prognostic factor reflecting immune condition of tumor microenvironment in squamous cell lung cancer
title_short Neutrophil-to-lymphocyte ratio is a prognostic factor reflecting immune condition of tumor microenvironment in squamous cell lung cancer
title_sort neutrophil to lymphocyte ratio is a prognostic factor reflecting immune condition of tumor microenvironment in squamous cell lung cancer
url https://doi.org/10.1038/s41598-023-50378-9
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