Gold Nanorod Bioconjugates for Active Tumor Targeting and Photothermal Therapy
The mastery of active tumor targeting is a great challenge in near infrared photothermal therapy (NIRPTT). To improve efficiency for targeted treatment of malignant tumors, we modify the technique of conjugating gold nanoparticles to tumor-specific antibodies. Polyethylene glycol-coated (PEGylated)...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Journal of Nanotechnology |
| Online Access: | http://dx.doi.org/10.1155/2011/631753 |
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| author | Hadiyah N. Green Dmitry V. Martyshkin Cynthia M. Rodenburg Eben L. Rosenthal Sergey B. Mirov |
| author_facet | Hadiyah N. Green Dmitry V. Martyshkin Cynthia M. Rodenburg Eben L. Rosenthal Sergey B. Mirov |
| author_sort | Hadiyah N. Green |
| collection | DOAJ |
| description | The mastery of active tumor targeting is a great challenge in near infrared photothermal therapy (NIRPTT). To improve efficiency for targeted treatment of malignant tumors, we modify the technique of conjugating gold nanoparticles to tumor-specific antibodies. Polyethylene glycol-coated (PEGylated) gold nanorods (GNRs) were fabricated and conjugated to an anti-EGFR antibody. We characterized the conjugation efficiency of the GNRs by comparing the efficiency of antibody binding and the photothermal effect of the GNRs before and after conjugation. We demonstrate that the binding efficiency of the antibodies conjugated to the PEGylated GNRs is comparable to the binding efficiency of the unmodified antibodies and 33.9% greater than PEGylated antibody-GNR conjugates as reported by Liao and Hafner (2005). In addition, cell death by NIRPTT was sufficient to kill nearly 90% of tumor cells, which is comparable to NIRPTT with GNRs alone confirming that NIRPTT using GNRs is not compromised by conjugation of GNRs to antibodies. |
| format | Article |
| id | doaj-art-406a86108c6544af81edddd65d52a086 |
| institution | Kabale University |
| issn | 1687-9503 1687-9511 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nanotechnology |
| spelling | doaj-art-406a86108c6544af81edddd65d52a0862025-02-03T05:59:00ZengWileyJournal of Nanotechnology1687-95031687-95112011-01-01201110.1155/2011/631753631753Gold Nanorod Bioconjugates for Active Tumor Targeting and Photothermal TherapyHadiyah N. Green0Dmitry V. Martyshkin1Cynthia M. Rodenburg2Eben L. Rosenthal3Sergey B. Mirov4Center for Optical Sensors and Spectroscopies and the Department of Physics, The University of Alabama at Birmingham, Campbell Hall 310, 1300 University Boulevard, Birmingham, AL 35294, USACenter for Optical Sensors and Spectroscopies and the Department of Physics, The University of Alabama at Birmingham, Campbell Hall 310, 1300 University Boulevard, Birmingham, AL 35294, USADepartment of Microbiology, The University of Alabama at Birmingham, Bevill Biomedical Research Building, 845 19th Street South, Birmingham, AL 35294, USADivision of Otolaryngology, Head and Neck Surgery, Department of Surgery, The University of Alabama at Birmingham, Volker Hall G082, 1670 University Boulevard, Birmingham, AL 35233, USACenter for Optical Sensors and Spectroscopies and the Department of Physics, The University of Alabama at Birmingham, Campbell Hall 310, 1300 University Boulevard, Birmingham, AL 35294, USAThe mastery of active tumor targeting is a great challenge in near infrared photothermal therapy (NIRPTT). To improve efficiency for targeted treatment of malignant tumors, we modify the technique of conjugating gold nanoparticles to tumor-specific antibodies. Polyethylene glycol-coated (PEGylated) gold nanorods (GNRs) were fabricated and conjugated to an anti-EGFR antibody. We characterized the conjugation efficiency of the GNRs by comparing the efficiency of antibody binding and the photothermal effect of the GNRs before and after conjugation. We demonstrate that the binding efficiency of the antibodies conjugated to the PEGylated GNRs is comparable to the binding efficiency of the unmodified antibodies and 33.9% greater than PEGylated antibody-GNR conjugates as reported by Liao and Hafner (2005). In addition, cell death by NIRPTT was sufficient to kill nearly 90% of tumor cells, which is comparable to NIRPTT with GNRs alone confirming that NIRPTT using GNRs is not compromised by conjugation of GNRs to antibodies.http://dx.doi.org/10.1155/2011/631753 |
| spellingShingle | Hadiyah N. Green Dmitry V. Martyshkin Cynthia M. Rodenburg Eben L. Rosenthal Sergey B. Mirov Gold Nanorod Bioconjugates for Active Tumor Targeting and Photothermal Therapy Journal of Nanotechnology |
| title | Gold Nanorod Bioconjugates for Active Tumor Targeting and Photothermal Therapy |
| title_full | Gold Nanorod Bioconjugates for Active Tumor Targeting and Photothermal Therapy |
| title_fullStr | Gold Nanorod Bioconjugates for Active Tumor Targeting and Photothermal Therapy |
| title_full_unstemmed | Gold Nanorod Bioconjugates for Active Tumor Targeting and Photothermal Therapy |
| title_short | Gold Nanorod Bioconjugates for Active Tumor Targeting and Photothermal Therapy |
| title_sort | gold nanorod bioconjugates for active tumor targeting and photothermal therapy |
| url | http://dx.doi.org/10.1155/2011/631753 |
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