Thiol-Disulfide Homeostasis in Noise-Induced Hearing Loss in Rats
Background: This study was designed to assess if thiol-disulfide homeostasis could be used as diagnostic biomarker in noise-induced hearing loss (NIHL) in a laboratory animal model. Methods: The study was carried out with a total of 28 female albino rats in 4 groups: group 1 (control group) inclu...
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AVES
2024-11-01
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| Series: | Journal of International Advanced Otology |
| Online Access: | https://www.advancedotology.org/en/thiol-disulfide-homeostasis-in-noise-induced-hearing-loss-in-rats-131960 |
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| author | Enes Aydın Serpil Mungan Durankaya Osman Yilmaz Günay Kirkim Safiye Aktaş Salim Neşelioğlu Özcan Erel Yüksel Olgun Abdullah Dalgıç |
| author_facet | Enes Aydın Serpil Mungan Durankaya Osman Yilmaz Günay Kirkim Safiye Aktaş Salim Neşelioğlu Özcan Erel Yüksel Olgun Abdullah Dalgıç |
| author_sort | Enes Aydın |
| collection | DOAJ |
| description | Background: This study was designed to assess if thiol-disulfide homeostasis could be used as diagnostic biomarker in noise-induced hearing loss (NIHL) in a laboratory animal model.
Methods: The study was carried out with a total of 28 female albino rats in 4 groups: group 1 (control group) included rats that were not exposed to noise or any study treatment; in group 2, following noise exposure, rats received 2 mg of dexamethasone per kilogram of body weight via the intramuscular route for 5 days; in Group 3, rats were exposed to noise and received a saline solution for 5 days, in a volume (0.15 cc) matched to that of dexamethasone administered in group 2; and in group 4, rats were exposed to noise, and blood samples were collected during the early phase to assess thiol-disulfide homeostasis without administering any treatment. Rats in groups 2, 3, and 4 were exposed to 120 dB noise in the 4 kHz octave band for 4 hours. The auditory brainstem response (ABR) test was performed in all groups on day 1 after noise exposure and was repeated in groups 1, 2, and 3 on day 5. Auditory brainstem response thresholds were recorded at 8, 12, 16, 20, and 32 kHz frequencies. Groups 1, 2, and 3 rats were sacrificed on day 5, and group 4 rats were sacrificed by exsanguination on day 1 after noise exposure. Venous blood samples collected from the caudal vena cava were centrifuged and sent to the corresponding laboratory for thiol-disulfide homeostasis studies. After sacrificing the rats, the right and left temporal bones of each rat were removed and stained with hematoxylin eosin for histological studies to explore any pyknotic changes in spiral ganglion cells.
Results: Intergroup comparisons by frequency on day 5 of noise exposure showed statistically significantly lower responses in ABR measurements at 8 kHz, 12 kHz, and 16 kHz in group 2 compared to group 3 (P = .003, P=.006, and P=.002). Improvements were observed with dexamethasone administered for therapeutic purposes, particularly if the hearing loss was induced by low-frequency noise. In the assessment of the parameters of thiol-disulfide homeostasis, disulfide/native thiol and disulfide/total thiol ratios and ischemia-modified albumin (IMA) levels were higher in group 4 than in other groups, although only the differences between group 1 and group 4 reached statistical significance.
Conclusion: According to this study, thiol-disulfide homeostasis and IMA can be shown as diagnostic biomarkers in NIHL, especially in the early period. The results from our study suggest that these markers may be used as adjunctive diagnostic tools in NIHL, in addition to audiological studies. However, this issue can be clarified with further clinical studies. |
| format | Article |
| id | doaj-art-4052df4d333b4de3a6e8033e57ca9fe7 |
| institution | Kabale University |
| issn | 2148-3817 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | AVES |
| record_format | Article |
| series | Journal of International Advanced Otology |
| spelling | doaj-art-4052df4d333b4de3a6e8033e57ca9fe72025-01-30T07:16:34ZengAVESJournal of International Advanced Otology2148-38172024-11-0120646647110.5152/iao.2024.241555Thiol-Disulfide Homeostasis in Noise-Induced Hearing Loss in RatsEnes Aydın0https://orcid.org/0000-0003-3413-0535Serpil Mungan Durankaya1https://orcid.org/0000-0003-4170-5317Osman Yilmaz2https://orcid.org/0000-0001-7817-7576Günay Kirkim3https://orcid.org/0000-0003-4170-5317Safiye Aktaş4https://orcid.org/0000-0002-7658-5565Salim Neşelioğlu5https://orcid.org/0000-0002-0974-5717Özcan Erel6https://orcid.org/0000-0002-2996-3236Yüksel Olgun7https://orcid.org/0000-0003-1769-4224Abdullah Dalgıç8Clinic of Ear-Nose and Throat, İstanbul Silivri State Hospital, İstanbul, TürkiyeDepartment of Ear-Nose and Throat, Audiology Section, Dokuz Eylül University Institute of Health Sciences, İzmir, TürkiyeDepartment of Laboratory Animals, Dokuz Eylül University Institute of Health Sciences, İzmir, TürkiyeDepartment of Ear-Nose and Throat, Audiology Section, Dokuz Eylül University Institute of Health Sciences, İzmir, TürkiyeDepartment of Oncology, Dokuz Eylül University Institute of Health Sciences, İzmir, TürkiyeDepartment of Medical Biochemistry, Yıldırım Beyazıt University School of Medicine, Ankara, TürkiyeDepartment of Medical Biochemistry, Yıldırım Beyazıt University School of Medicine, Ankara, TürkiyeDepartment of Ear-Nose and Throat, Dokuz Eylül University School of Medicine, İzmir, Türkiyehttps://orcid.org/0000-0002-6958-4169Background: This study was designed to assess if thiol-disulfide homeostasis could be used as diagnostic biomarker in noise-induced hearing loss (NIHL) in a laboratory animal model. Methods: The study was carried out with a total of 28 female albino rats in 4 groups: group 1 (control group) included rats that were not exposed to noise or any study treatment; in group 2, following noise exposure, rats received 2 mg of dexamethasone per kilogram of body weight via the intramuscular route for 5 days; in Group 3, rats were exposed to noise and received a saline solution for 5 days, in a volume (0.15 cc) matched to that of dexamethasone administered in group 2; and in group 4, rats were exposed to noise, and blood samples were collected during the early phase to assess thiol-disulfide homeostasis without administering any treatment. Rats in groups 2, 3, and 4 were exposed to 120 dB noise in the 4 kHz octave band for 4 hours. The auditory brainstem response (ABR) test was performed in all groups on day 1 after noise exposure and was repeated in groups 1, 2, and 3 on day 5. Auditory brainstem response thresholds were recorded at 8, 12, 16, 20, and 32 kHz frequencies. Groups 1, 2, and 3 rats were sacrificed on day 5, and group 4 rats were sacrificed by exsanguination on day 1 after noise exposure. Venous blood samples collected from the caudal vena cava were centrifuged and sent to the corresponding laboratory for thiol-disulfide homeostasis studies. After sacrificing the rats, the right and left temporal bones of each rat were removed and stained with hematoxylin eosin for histological studies to explore any pyknotic changes in spiral ganglion cells. Results: Intergroup comparisons by frequency on day 5 of noise exposure showed statistically significantly lower responses in ABR measurements at 8 kHz, 12 kHz, and 16 kHz in group 2 compared to group 3 (P = .003, P=.006, and P=.002). Improvements were observed with dexamethasone administered for therapeutic purposes, particularly if the hearing loss was induced by low-frequency noise. In the assessment of the parameters of thiol-disulfide homeostasis, disulfide/native thiol and disulfide/total thiol ratios and ischemia-modified albumin (IMA) levels were higher in group 4 than in other groups, although only the differences between group 1 and group 4 reached statistical significance. Conclusion: According to this study, thiol-disulfide homeostasis and IMA can be shown as diagnostic biomarkers in NIHL, especially in the early period. The results from our study suggest that these markers may be used as adjunctive diagnostic tools in NIHL, in addition to audiological studies. However, this issue can be clarified with further clinical studies.https://www.advancedotology.org/en/thiol-disulfide-homeostasis-in-noise-induced-hearing-loss-in-rats-131960 |
| spellingShingle | Enes Aydın Serpil Mungan Durankaya Osman Yilmaz Günay Kirkim Safiye Aktaş Salim Neşelioğlu Özcan Erel Yüksel Olgun Abdullah Dalgıç Thiol-Disulfide Homeostasis in Noise-Induced Hearing Loss in Rats Journal of International Advanced Otology |
| title | Thiol-Disulfide Homeostasis in Noise-Induced Hearing Loss in Rats |
| title_full | Thiol-Disulfide Homeostasis in Noise-Induced Hearing Loss in Rats |
| title_fullStr | Thiol-Disulfide Homeostasis in Noise-Induced Hearing Loss in Rats |
| title_full_unstemmed | Thiol-Disulfide Homeostasis in Noise-Induced Hearing Loss in Rats |
| title_short | Thiol-Disulfide Homeostasis in Noise-Induced Hearing Loss in Rats |
| title_sort | thiol disulfide homeostasis in noise induced hearing loss in rats |
| url | https://www.advancedotology.org/en/thiol-disulfide-homeostasis-in-noise-induced-hearing-loss-in-rats-131960 |
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