Molecular characterization of feline caliciviruses isolated from several adult cats with atypical infection showing severe flu-like symptoms on a remote island in Ehime, Japan
In November 2020, a volunteer group reported an outbreak of an infectious disease with a high fatality rate and flu-like symptoms among stray cats in Aoshima, a remote island in Ehime, Japan. Nine adult cats with severe symptoms were hospitalized. Feline calicivirus (FCV) was isolated from pharyngea...
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Elsevier
2025-03-01
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| Series: | Virus Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0168170225000115 |
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| author | Yuki Nishisaka Hikaru Fujii Fumiko Ono Sho Kadekaru Hiroyuki Kogiku Yumi Une Shione Takeguchi Naomi Ohta Masumi Eto Chiharu Takeuchi Seigou Takeuchi Tetsuko Miki Akihiko Tokuda Keiko Ookawa Yukinobu Tohya Keita Ishijima Akiko Okutani Ken Maeda Shumpei Watanabe Shigeru Morikawa |
| author_facet | Yuki Nishisaka Hikaru Fujii Fumiko Ono Sho Kadekaru Hiroyuki Kogiku Yumi Une Shione Takeguchi Naomi Ohta Masumi Eto Chiharu Takeuchi Seigou Takeuchi Tetsuko Miki Akihiko Tokuda Keiko Ookawa Yukinobu Tohya Keita Ishijima Akiko Okutani Ken Maeda Shumpei Watanabe Shigeru Morikawa |
| author_sort | Yuki Nishisaka |
| collection | DOAJ |
| description | In November 2020, a volunteer group reported an outbreak of an infectious disease with a high fatality rate and flu-like symptoms among stray cats in Aoshima, a remote island in Ehime, Japan. Nine adult cats with severe symptoms were hospitalized. Feline calicivirus (FCV) was isolated from pharyngeal swabs of six hospitalized cats. An outbreak of virulent systemic FCV (VS-FCV) infection was initially suspected because of obvious flu-like symptoms in adult cats; however, no symptoms typically associated with VS-FCV, such as skin ulcers on the limbs, edema, or viremia, were observed. Notably, two of the hospitalized cats that showed severe disease had diarrhea and anemia, and died or had a prolonged illness. These cases reveal atypical symptoms of FCV infection that have not been previously reported. We further isolated typical strains from western Japan (Osaka, Kumamoto, and Ehime) and analyzed the viral genes along with virulent strains from Aoshima. Phylogenetic analysis showed that the Aoshima strain formed a new lineage distinct from known FCVs. The Aoshima strains isolated in the initial outbreak before December 5, 2020, and those isolated after the end of the outbreak, which are suspected pathogenic and typical non-pathogenic strains, respectively, were located in the same cluster and shown to be very similar in sequence. The virulent Aoshima strain, which causes atypical FCV infections in cats, may have been derived by acquiring several mutations from a typical strain that chronically infects cats on a remote island. |
| format | Article |
| id | doaj-art-404d6ecab0cd46c980c9bb3b0095ccca |
| institution | Kabale University |
| issn | 1872-7492 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Virus Research |
| spelling | doaj-art-404d6ecab0cd46c980c9bb3b0095ccca2025-01-31T05:10:03ZengElsevierVirus Research1872-74922025-03-01353199535Molecular characterization of feline caliciviruses isolated from several adult cats with atypical infection showing severe flu-like symptoms on a remote island in Ehime, JapanYuki Nishisaka0Hikaru Fujii1Fumiko Ono2Sho Kadekaru3Hiroyuki Kogiku4Yumi Une5Shione Takeguchi6Naomi Ohta7Masumi Eto8Chiharu Takeuchi9Seigou Takeuchi10Tetsuko Miki11Akihiko Tokuda12Keiko Ookawa13Yukinobu Tohya14Keita Ishijima15Akiko Okutani16Ken Maeda17Shumpei Watanabe18Shigeru Morikawa19Department of Microbiology, Faculty of Veterinary Medicine, Okayama University of Science, Imabari, Ehime, JapanDepartment of Microbiology, Faculty of Veterinary Medicine, Okayama University of Science, Imabari, Ehime, JapanLaboratory Animal Science, Faculty of Veterinary Medicine, Okayama University of Science, Imabari, Ehime, Japan; Corresponding author.Laboratory of Veterinary Pathology, Faculty of Veterinary Medicine, Okayama University of Science, Imabari, Ehime, JapanLaboratory of Veterinary Pathology, Faculty of Veterinary Medicine, Okayama University of Science, Imabari, Ehime, JapanLaboratory of Veterinary Pathology, Faculty of Veterinary Medicine, Okayama University of Science, Imabari, Ehime, JapanLaboratory Animal Science, Faculty of Veterinary Medicine, Okayama University of Science, Imabari, Ehime, JapanDepartment of Epidemiology, Faculty of Veterinary Medicine, Okayama University of Science, Imabari, Ehime, JapanBiochemistry Unit, Faculty of Veterinary Medicine, Okayama University of Science, Imabari, Ehime, JapanOzuMorino Animal Hospital, Ozu, Ehime, JapanOzuMorino Animal Hospital, Ozu, Ehime, JapanDaktari Animal Hospital Kansai Medical Center, Sakai, Osaka, JapanRyunosuke Animal Hospital, Kumamoto, Kumamoto, JapanRyunosuke Animal Hospital, Kumamoto, Kumamoto, JapanLaboratory of Veterinary Microbiology, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, JapanDepartment of Veterinary Science, National Institute of Infectious Diseases, Shinjuku, Tokyo, JapanDepartment of Veterinary Science, National Institute of Infectious Diseases, Shinjuku, Tokyo, JapanDepartment of Veterinary Science, National Institute of Infectious Diseases, Shinjuku, Tokyo, JapanDepartment of Microbiology, Faculty of Veterinary Medicine, Okayama University of Science, Imabari, Ehime, Japan; Corresponding author.Department of Microbiology, Faculty of Veterinary Medicine, Okayama University of Science, Imabari, Ehime, JapanIn November 2020, a volunteer group reported an outbreak of an infectious disease with a high fatality rate and flu-like symptoms among stray cats in Aoshima, a remote island in Ehime, Japan. Nine adult cats with severe symptoms were hospitalized. Feline calicivirus (FCV) was isolated from pharyngeal swabs of six hospitalized cats. An outbreak of virulent systemic FCV (VS-FCV) infection was initially suspected because of obvious flu-like symptoms in adult cats; however, no symptoms typically associated with VS-FCV, such as skin ulcers on the limbs, edema, or viremia, were observed. Notably, two of the hospitalized cats that showed severe disease had diarrhea and anemia, and died or had a prolonged illness. These cases reveal atypical symptoms of FCV infection that have not been previously reported. We further isolated typical strains from western Japan (Osaka, Kumamoto, and Ehime) and analyzed the viral genes along with virulent strains from Aoshima. Phylogenetic analysis showed that the Aoshima strain formed a new lineage distinct from known FCVs. The Aoshima strains isolated in the initial outbreak before December 5, 2020, and those isolated after the end of the outbreak, which are suspected pathogenic and typical non-pathogenic strains, respectively, were located in the same cluster and shown to be very similar in sequence. The virulent Aoshima strain, which causes atypical FCV infections in cats, may have been derived by acquiring several mutations from a typical strain that chronically infects cats on a remote island.http://www.sciencedirect.com/science/article/pii/S0168170225000115Feline calicivirusVirulent systemic feline calicivirusVP1Feline infectious diseaseUpper respiratory tract diseaseSevere flu-like symptom |
| spellingShingle | Yuki Nishisaka Hikaru Fujii Fumiko Ono Sho Kadekaru Hiroyuki Kogiku Yumi Une Shione Takeguchi Naomi Ohta Masumi Eto Chiharu Takeuchi Seigou Takeuchi Tetsuko Miki Akihiko Tokuda Keiko Ookawa Yukinobu Tohya Keita Ishijima Akiko Okutani Ken Maeda Shumpei Watanabe Shigeru Morikawa Molecular characterization of feline caliciviruses isolated from several adult cats with atypical infection showing severe flu-like symptoms on a remote island in Ehime, Japan Virus Research Feline calicivirus Virulent systemic feline calicivirus VP1 Feline infectious disease Upper respiratory tract disease Severe flu-like symptom |
| title | Molecular characterization of feline caliciviruses isolated from several adult cats with atypical infection showing severe flu-like symptoms on a remote island in Ehime, Japan |
| title_full | Molecular characterization of feline caliciviruses isolated from several adult cats with atypical infection showing severe flu-like symptoms on a remote island in Ehime, Japan |
| title_fullStr | Molecular characterization of feline caliciviruses isolated from several adult cats with atypical infection showing severe flu-like symptoms on a remote island in Ehime, Japan |
| title_full_unstemmed | Molecular characterization of feline caliciviruses isolated from several adult cats with atypical infection showing severe flu-like symptoms on a remote island in Ehime, Japan |
| title_short | Molecular characterization of feline caliciviruses isolated from several adult cats with atypical infection showing severe flu-like symptoms on a remote island in Ehime, Japan |
| title_sort | molecular characterization of feline caliciviruses isolated from several adult cats with atypical infection showing severe flu like symptoms on a remote island in ehime japan |
| topic | Feline calicivirus Virulent systemic feline calicivirus VP1 Feline infectious disease Upper respiratory tract disease Severe flu-like symptom |
| url | http://www.sciencedirect.com/science/article/pii/S0168170225000115 |
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