Effects of carbon sources and temperature on the formation and structural characteristics of food-related Staphylococcus epidermidis biofilms
Biofilms are a constant concern in the food industry; understanding the effect of environmental conditions on biofilm formation is essential to develop effective control strategies. Therefore, this study was conducted to investigate biofilms formation by Staphylococcus epidermidis under various cond...
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Tsinghua University Press
2020-12-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453020301506 |
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| author | Mingming Zou Donghong Liu |
| author_facet | Mingming Zou Donghong Liu |
| author_sort | Mingming Zou |
| collection | DOAJ |
| description | Biofilms are a constant concern in the food industry; understanding the effect of environmental conditions on biofilm formation is essential to develop effective control strategies. Therefore, this study was conducted to investigate biofilms formation by Staphylococcus epidermidis under various conditions. Biofilms were cultured in nutrient broth containing different carbon source concentrations (0–10 mg/mL) on polystyrene surfaces for 32 h of incubation at 37 °C or 55 °C, with quantification and enumeration at 8, 16, 24 and 32 h. S. epidermidis developed biofilms under all tested conditions; achieved the highest yield of biofilm biomass at 2.5 mg/mL for all carbon sources at 37 °C. The highest efficiency of extracellular polymeric substance (EPS) molecule production occurred under glucose availability in the growth environment, with a higher yield of biomass and a significantly smaller number of metabolically active cells than under other tested conditions. A condensed ball-shaped structure was observed under the lactose condition. Meanwhile, biofilms in the presence of maltose showed mainly opaque thick rich colonies, while a compact multilayered-shaped structure was exhibited under both glucose and sucrose conditions. These results contribute to a better understanding of the biofilm formation by S. epidermidis in order to reduce contamination and recontamination in the food industry. |
| format | Article |
| id | doaj-art-404903aaeec24bbda4a59fe58d73b0dc |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2020-12-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-404903aaeec24bbda4a59fe58d73b0dc2025-02-03T04:07:26ZengTsinghua University PressFood Science and Human Wellness2213-45302020-12-0194370376Effects of carbon sources and temperature on the formation and structural characteristics of food-related Staphylococcus epidermidis biofilmsMingming Zou0Donghong Liu1College of Biosystems Engineering and Food Science, National-Local Joint Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang Engineering Laboratory of Food Technology and Equipment, Zhejiang University, Hangzhou 310058, ChinaCollege of Biosystems Engineering and Food Science, National-Local Joint Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang Engineering Laboratory of Food Technology and Equipment, Zhejiang University, Hangzhou 310058, China; Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058, China; Ningbo Research Institute, Zhejiang University, Ningbo 315100, China; Corresponding author at: College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Rd., Hangzhou, Zhejiang, 310058, China.Biofilms are a constant concern in the food industry; understanding the effect of environmental conditions on biofilm formation is essential to develop effective control strategies. Therefore, this study was conducted to investigate biofilms formation by Staphylococcus epidermidis under various conditions. Biofilms were cultured in nutrient broth containing different carbon source concentrations (0–10 mg/mL) on polystyrene surfaces for 32 h of incubation at 37 °C or 55 °C, with quantification and enumeration at 8, 16, 24 and 32 h. S. epidermidis developed biofilms under all tested conditions; achieved the highest yield of biofilm biomass at 2.5 mg/mL for all carbon sources at 37 °C. The highest efficiency of extracellular polymeric substance (EPS) molecule production occurred under glucose availability in the growth environment, with a higher yield of biomass and a significantly smaller number of metabolically active cells than under other tested conditions. A condensed ball-shaped structure was observed under the lactose condition. Meanwhile, biofilms in the presence of maltose showed mainly opaque thick rich colonies, while a compact multilayered-shaped structure was exhibited under both glucose and sucrose conditions. These results contribute to a better understanding of the biofilm formation by S. epidermidis in order to reduce contamination and recontamination in the food industry.http://www.sciencedirect.com/science/article/pii/S2213453020301506Staphylococcus epidermidisCarbon sourcesTemperatureBacterial biofilmCommunity structure |
| spellingShingle | Mingming Zou Donghong Liu Effects of carbon sources and temperature on the formation and structural characteristics of food-related Staphylococcus epidermidis biofilms Food Science and Human Wellness Staphylococcus epidermidis Carbon sources Temperature Bacterial biofilm Community structure |
| title | Effects of carbon sources and temperature on the formation and structural characteristics of food-related Staphylococcus epidermidis biofilms |
| title_full | Effects of carbon sources and temperature on the formation and structural characteristics of food-related Staphylococcus epidermidis biofilms |
| title_fullStr | Effects of carbon sources and temperature on the formation and structural characteristics of food-related Staphylococcus epidermidis biofilms |
| title_full_unstemmed | Effects of carbon sources and temperature on the formation and structural characteristics of food-related Staphylococcus epidermidis biofilms |
| title_short | Effects of carbon sources and temperature on the formation and structural characteristics of food-related Staphylococcus epidermidis biofilms |
| title_sort | effects of carbon sources and temperature on the formation and structural characteristics of food related staphylococcus epidermidis biofilms |
| topic | Staphylococcus epidermidis Carbon sources Temperature Bacterial biofilm Community structure |
| url | http://www.sciencedirect.com/science/article/pii/S2213453020301506 |
| work_keys_str_mv | AT mingmingzou effectsofcarbonsourcesandtemperatureontheformationandstructuralcharacteristicsoffoodrelatedstaphylococcusepidermidisbiofilms AT donghongliu effectsofcarbonsourcesandtemperatureontheformationandstructuralcharacteristicsoffoodrelatedstaphylococcusepidermidisbiofilms |