Evaluation of safe exposure time for two-photon microscopy imaging of acrylic-painted mock-ups
Nonlinear optical microscopies are widely used in the biological and biomedical fields, as they are non-invasive techniques that permit the safe structural and morphological characterisation of cells and tissues. They are increasingly being used in the Cultural Heritage field because of their abilit...
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| Format: | Article |
| Language: | English |
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IOP Publishing
2025-01-01
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| Series: | JPhys Photonics |
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| Online Access: | https://doi.org/10.1088/2515-7647/ada998 |
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| author | Sara Mattana Alice Dal Fovo Enrico Baria Raffaella Fontana Riccardo Cicchi |
| author_facet | Sara Mattana Alice Dal Fovo Enrico Baria Raffaella Fontana Riccardo Cicchi |
| author_sort | Sara Mattana |
| collection | DOAJ |
| description | Nonlinear optical microscopies are widely used in the biological and biomedical fields, as they are non-invasive techniques that permit the safe structural and morphological characterisation of cells and tissues. They are increasingly being used in the Cultural Heritage field because of their ability to overcome some limits of the well-established optical techniques. However, since nonlinear optical microscopies use pulsed laser sources with high peak power, their application in Cultural Heritage raises concerns due to artworks’ unique, priceless, and delicate nature. In this paper, we present a new method for evaluating the photo-induced damage when using a near-infrared femtosecond pulsed laser to perform two-photon excited fluorescence imaging of acrylic-painted mock-ups. In particular, we explore herein several irradiation conditions, varying the exposure time and excitation power, in order to provide useful experimental indications for safely imaging acrylic paints with two-photon microscopy. |
| format | Article |
| id | doaj-art-40254e47a31f4d7ea66650ab34cc71e6 |
| institution | Kabale University |
| issn | 2515-7647 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | IOP Publishing |
| record_format | Article |
| series | JPhys Photonics |
| spelling | doaj-art-40254e47a31f4d7ea66650ab34cc71e62025-02-04T14:14:36ZengIOP PublishingJPhys Photonics2515-76472025-01-017202500110.1088/2515-7647/ada998Evaluation of safe exposure time for two-photon microscopy imaging of acrylic-painted mock-upsSara Mattana0https://orcid.org/0000-0002-6619-0017Alice Dal Fovo1https://orcid.org/0000-0001-7960-2546Enrico Baria2https://orcid.org/0000-0001-9679-7736Raffaella Fontana3https://orcid.org/0000-0002-7663-1277Riccardo Cicchi4https://orcid.org/0000-0002-2583-6694Consiglio Nazionale delle Ricerche—Istituto Nazionale di Ottica (CNR-INO) , Largo Enrico Fermi 6, 50125 Firenze, ItalyConsiglio Nazionale delle Ricerche—Istituto Nazionale di Ottica (CNR-INO) , Largo Enrico Fermi 6, 50125 Firenze, ItalyConsiglio Nazionale delle Ricerche—Istituto Nazionale di Ottica (CNR-INO) , Largo Enrico Fermi 6, 50125 Firenze, Italy; Department of Physics and Astronomy, University of Florence , Via G. Sansone 1, 50019 Sesto Fiorentino, ItalyConsiglio Nazionale delle Ricerche—Istituto Nazionale di Ottica (CNR-INO) , Largo Enrico Fermi 6, 50125 Firenze, ItalyConsiglio Nazionale delle Ricerche—Istituto Nazionale di Ottica (CNR-INO) , Largo Enrico Fermi 6, 50125 Firenze, Italy; European Laboratory for Non-linear Spectroscopy (LENS) , Via Nello Carrara 1, 50019 Sesto Fiorentino, ItalyNonlinear optical microscopies are widely used in the biological and biomedical fields, as they are non-invasive techniques that permit the safe structural and morphological characterisation of cells and tissues. They are increasingly being used in the Cultural Heritage field because of their ability to overcome some limits of the well-established optical techniques. However, since nonlinear optical microscopies use pulsed laser sources with high peak power, their application in Cultural Heritage raises concerns due to artworks’ unique, priceless, and delicate nature. In this paper, we present a new method for evaluating the photo-induced damage when using a near-infrared femtosecond pulsed laser to perform two-photon excited fluorescence imaging of acrylic-painted mock-ups. In particular, we explore herein several irradiation conditions, varying the exposure time and excitation power, in order to provide useful experimental indications for safely imaging acrylic paints with two-photon microscopy.https://doi.org/10.1088/2515-7647/ada998nonlinear optical microscopiestwo-photon excited fluorescencephoto-damagingacrylic paintspeak power |
| spellingShingle | Sara Mattana Alice Dal Fovo Enrico Baria Raffaella Fontana Riccardo Cicchi Evaluation of safe exposure time for two-photon microscopy imaging of acrylic-painted mock-ups JPhys Photonics nonlinear optical microscopies two-photon excited fluorescence photo-damaging acrylic paints peak power |
| title | Evaluation of safe exposure time for two-photon microscopy imaging of acrylic-painted mock-ups |
| title_full | Evaluation of safe exposure time for two-photon microscopy imaging of acrylic-painted mock-ups |
| title_fullStr | Evaluation of safe exposure time for two-photon microscopy imaging of acrylic-painted mock-ups |
| title_full_unstemmed | Evaluation of safe exposure time for two-photon microscopy imaging of acrylic-painted mock-ups |
| title_short | Evaluation of safe exposure time for two-photon microscopy imaging of acrylic-painted mock-ups |
| title_sort | evaluation of safe exposure time for two photon microscopy imaging of acrylic painted mock ups |
| topic | nonlinear optical microscopies two-photon excited fluorescence photo-damaging acrylic paints peak power |
| url | https://doi.org/10.1088/2515-7647/ada998 |
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