De novo abnormalities identified by fluorescence in situ hybridization during follow-up confer poor prognosis in Chinese multiple myeloma

BackgroundAlthough there is evolving consensus to re-evaluate cytogenetic features during follow-up in multiple myeloma (MM), longitudinal studies on cytogenetic evolution in Chinese MM patients are still lacking. Our aim was to highlight the importance of ongoing monitoring of cytogenetic character...

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Main Authors: Shumin Chen, Lu Gao, Lin Feng, Zheng Wang, Ye Li, Qing Liu, Wenjie Song, Shu Kong, Yang Liu, Jin Lu, Yingjun Chang, Xiaojun Huang, Yueyun Lai
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-03-01
Series:Frontiers in Medicine
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Online Access:https://www.frontiersin.org/articles/10.3389/fmed.2025.1536825/full
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author Shumin Chen
Lu Gao
Lin Feng
Zheng Wang
Ye Li
Qing Liu
Wenjie Song
Shu Kong
Yang Liu
Jin Lu
Yingjun Chang
Xiaojun Huang
Yueyun Lai
author_facet Shumin Chen
Lu Gao
Lin Feng
Zheng Wang
Ye Li
Qing Liu
Wenjie Song
Shu Kong
Yang Liu
Jin Lu
Yingjun Chang
Xiaojun Huang
Yueyun Lai
author_sort Shumin Chen
collection DOAJ
description BackgroundAlthough there is evolving consensus to re-evaluate cytogenetic features during follow-up in multiple myeloma (MM), longitudinal studies on cytogenetic evolution in Chinese MM patients are still lacking. Our aim was to highlight the importance of ongoing monitoring of cytogenetic characteristics and shed light on the implications of clonal evolution in Chinese MM patients.Patients and methodsThe clinical data of 230 MM patients were retrospectively analyzed, including 100 patients were continuously monitored for cytogenetic abnormalities by fluorescence in situ hybridization (FISH).Results49 out of 100 patients acquired de novo FISH abnormalities during follow-up, which were associated with disease progression (p = 0.003) and inferior progression free survival (PFS) (median 31 vs. 51 months, p = 0.032). Patients with ≥2 de novo FISH abnormalities had poorer PFS (median 24 vs. 45 months, p = 0.003) when compared to those with l or no de novo FISH abnormality. Patients who acquired new abnormalities within 31 months since diagnosis had significantly worse PFS (median: 20 vs. 41 months, p < 0.001) and Overall Survival (OS) (median: 61 vs. 100 months, p = 0.008) compared to those who acquired new abnormalities after 31 months. When gain/amp 1q21, del(17p), t(4;14), and t(14;16) were classified as high risk abnormalities (HRA), patients with ≥2 HRA had a shorter PFS (median 28 vs. 49 months, p = 0.038) and OS (median 75 vs. 107 months, p = 0.040) when compared to those without HRA.ConclusionRe-evaluation of cytogenetic characteristics by serial FISH tests is important in MM patients. De novo FISH abnormalities during follow-up are adverse prognostic factors, especially when ≥2 new FISH anomalies and acquired new abnormalities within 31 months since diagnosis are presented, and the presence of ≥2 HRA during the disease process are associated with poor survival in Chinese MM patients.
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spelling doaj-art-401cf4d03bc54c2092ed878c35c94eb32025-08-20T02:58:37ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2025-03-011210.3389/fmed.2025.15368251536825De novo abnormalities identified by fluorescence in situ hybridization during follow-up confer poor prognosis in Chinese multiple myelomaShumin ChenLu GaoLin FengZheng WangYe LiQing LiuWenjie SongShu KongYang LiuJin LuYingjun ChangXiaojun HuangYueyun LaiBackgroundAlthough there is evolving consensus to re-evaluate cytogenetic features during follow-up in multiple myeloma (MM), longitudinal studies on cytogenetic evolution in Chinese MM patients are still lacking. Our aim was to highlight the importance of ongoing monitoring of cytogenetic characteristics and shed light on the implications of clonal evolution in Chinese MM patients.Patients and methodsThe clinical data of 230 MM patients were retrospectively analyzed, including 100 patients were continuously monitored for cytogenetic abnormalities by fluorescence in situ hybridization (FISH).Results49 out of 100 patients acquired de novo FISH abnormalities during follow-up, which were associated with disease progression (p = 0.003) and inferior progression free survival (PFS) (median 31 vs. 51 months, p = 0.032). Patients with ≥2 de novo FISH abnormalities had poorer PFS (median 24 vs. 45 months, p = 0.003) when compared to those with l or no de novo FISH abnormality. Patients who acquired new abnormalities within 31 months since diagnosis had significantly worse PFS (median: 20 vs. 41 months, p < 0.001) and Overall Survival (OS) (median: 61 vs. 100 months, p = 0.008) compared to those who acquired new abnormalities after 31 months. When gain/amp 1q21, del(17p), t(4;14), and t(14;16) were classified as high risk abnormalities (HRA), patients with ≥2 HRA had a shorter PFS (median 28 vs. 49 months, p = 0.038) and OS (median 75 vs. 107 months, p = 0.040) when compared to those without HRA.ConclusionRe-evaluation of cytogenetic characteristics by serial FISH tests is important in MM patients. De novo FISH abnormalities during follow-up are adverse prognostic factors, especially when ≥2 new FISH anomalies and acquired new abnormalities within 31 months since diagnosis are presented, and the presence of ≥2 HRA during the disease process are associated with poor survival in Chinese MM patients.https://www.frontiersin.org/articles/10.3389/fmed.2025.1536825/fullmultiple myelomasurvivalhigh-riskclonal evolutioncytogenetics
spellingShingle Shumin Chen
Lu Gao
Lin Feng
Zheng Wang
Ye Li
Qing Liu
Wenjie Song
Shu Kong
Yang Liu
Jin Lu
Yingjun Chang
Xiaojun Huang
Yueyun Lai
De novo abnormalities identified by fluorescence in situ hybridization during follow-up confer poor prognosis in Chinese multiple myeloma
Frontiers in Medicine
multiple myeloma
survival
high-risk
clonal evolution
cytogenetics
title De novo abnormalities identified by fluorescence in situ hybridization during follow-up confer poor prognosis in Chinese multiple myeloma
title_full De novo abnormalities identified by fluorescence in situ hybridization during follow-up confer poor prognosis in Chinese multiple myeloma
title_fullStr De novo abnormalities identified by fluorescence in situ hybridization during follow-up confer poor prognosis in Chinese multiple myeloma
title_full_unstemmed De novo abnormalities identified by fluorescence in situ hybridization during follow-up confer poor prognosis in Chinese multiple myeloma
title_short De novo abnormalities identified by fluorescence in situ hybridization during follow-up confer poor prognosis in Chinese multiple myeloma
title_sort de novo abnormalities identified by fluorescence in situ hybridization during follow up confer poor prognosis in chinese multiple myeloma
topic multiple myeloma
survival
high-risk
clonal evolution
cytogenetics
url https://www.frontiersin.org/articles/10.3389/fmed.2025.1536825/full
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