Phenotyping determination of cyp1a2 enzyme activity using caffeine in sheep
<b>Aim:</b> The aims of this study were to determine the validity of the plasma metabolic ratios (MR) to investigate the CYP1A2 activity in practice and to compare in vivo CYP1A2 enzyme activity using caffeine (CF) as a probe in Morkaraman (MK), Akkaraman (AK) and Anatolia Merino (AM) sh...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=543 |
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| Summary: | <b>Aim:</b> The aims of this study were to determine the validity of
the plasma metabolic ratios (MR) to investigate the CYP1A2
activity in practice and to compare in vivo CYP1A2 enzyme
activity using caffeine (CF) as a probe in Morkaraman (MK),
Akkaraman (AK) and Anatolia Merino (AM) sheep breeds.<p>
<b>Materials and Methods:</b> Caffeine was administered as a
single dose of 5 mg/kg b.w. by the intravenous in MK, AK
and AM sheep breeds. The plasma levels of CF and paraxanthine
(PX) were measured using high-performance liquid
chromatography. CYP1A2 phenotyping was measured using
the ratio [(PX/CF)AUC] between areas under the plasma
concentration-time curve (AUCs) of PX and CF and the ratios
[(PX/CF)MR→3-16 h] between plasma concentrations of
PX and CF at 3 to 16 h after CF administration. Correlations
between the plasma (PX/CF)MR→3-16 h and (PX/CF)AUC ratios
were determined to investigate of the CYP1A2 phenotyping
by single blood sampling in practice.<p>
<b>Results:</b> It was determined that the more reliable sampling
time within the plasma (PX/CF)MR→3-16 h ratios in the determination
of the CYP1A2 phenotyping was 10 h after CF
administration. (PX/CF)MR→10 h and (PX/CF)AUC ratios were
similar (p>0.05) among sheep breeds.<p>
<b>Conclusion:</b> The plasma (PX/CF)MR→10 h ratio might be used
as a rapid and simple screening test for CYP1A2 phenotyping
in sheep. CYP1A2 enzyme may not be clinically important
in the observed differences to the effects of drugs and
environmental chemicals with its substrates among MK, AK
and AM sheep breeds. |
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| ISSN: | 1309-6958 2146-1953 |