Stimulation of MMP-1 and CCL2 by NAMPT in PDL Cells
Periodontitis is an inflammatory disease caused by pathogenic microorganisms and characterized by the destruction of the periodontium. Obese individuals have an increased risk of periodontitis, and elevated circulating levels of adipokines, such as nicotinamide phosphoribosyltransferase (NAMPT), may...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2013/437123 |
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| author | Marjan Nokhbehsaim Sigrun Eick Andressa Vilas Boas Nogueira Per Hoffmann Stefan Herms Holger Fröhlich Søren Jepsen Andreas Jäger Joni Augusto Cirelli James Deschner |
| author_facet | Marjan Nokhbehsaim Sigrun Eick Andressa Vilas Boas Nogueira Per Hoffmann Stefan Herms Holger Fröhlich Søren Jepsen Andreas Jäger Joni Augusto Cirelli James Deschner |
| author_sort | Marjan Nokhbehsaim |
| collection | DOAJ |
| description | Periodontitis is an inflammatory disease caused by pathogenic microorganisms and characterized by the destruction of the periodontium. Obese individuals have an increased risk of periodontitis, and elevated circulating levels of adipokines, such as nicotinamide phosphoribosyltransferase (NAMPT), may be a pathomechanistic link between both diseases. The aim of this in vitro study was to examine the regulation of periodontal ligament (PDL) cells by NAMPT and its production under inflammatory and infectious conditions. NAMPT caused a significant upregulation of 9 genes and downregulation of 3 genes, as analyzed by microarray analysis. Eight of these genes could be confirmed by real-time PCR: NAMPT induced a significant upregulation of EGR1, MMP-1, SYT7, ITPKA, CCL2, NTM, IGF2BP3, and NRP1. NAMPT also increased significantly the MMP-1 and CCL2 protein synthesis. NAMPT was significantly induced by interleukin-1β and the periodontal microorganism P. gingivalis. NAMPT may contribute to periodontitis through upregulation of MMP-1 and CCL2 in PDL cells. Increased NAMPT levels, as found in obesity, may therefore represent a mechanism whereby obesity could confer an increased risk of periodontitis. Furthermore, microbial and inflammatory signals may enhance the NAMPT synthesis in PDL cells and thereby contribute to the increased gingival and serum levels of this adipokine, as found in periodontitis. |
| format | Article |
| id | doaj-art-4018f37e5d214977b2975e56885949a1 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-4018f37e5d214977b2975e56885949a12025-02-03T01:26:45ZengWileyMediators of Inflammation0962-93511466-18612013-01-01201310.1155/2013/437123437123Stimulation of MMP-1 and CCL2 by NAMPT in PDL CellsMarjan Nokhbehsaim0Sigrun Eick1Andressa Vilas Boas Nogueira2Per Hoffmann3Stefan Herms4Holger Fröhlich5Søren Jepsen6Andreas Jäger7Joni Augusto Cirelli8James Deschner9Experimental Dento-Maxillo-Facial Medicine, Center of Dento-Maxillo-Facial Medicine, University of Bonn, 53111 Bonn, GermanyDepartment of Periodontology, Laboratory of Oral Microbiology, University of Bern, 3010 Bern, SwitzerlandExperimental Dento-Maxillo-Facial Medicine, Center of Dento-Maxillo-Facial Medicine, University of Bonn, 53111 Bonn, GermanyInstitute of Human Genetics, Biomedical Center, University of Bonn, 53127 Bonn, GermanyInstitute of Human Genetics, Biomedical Center, University of Bonn, 53127 Bonn, GermanyBonn-Aachen International Center for IT, Algorithmic Bioinformatics, University of Bonn, 53113 Bonn, GermanyClinical Research Unit 208, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Welschnonnenstraße 17, 53111 Bonn, GermanyClinical Research Unit 208, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Welschnonnenstraße 17, 53111 Bonn, GermanyDepartment of Diagnosis and Surgery, School of Dentistry, SP, UNESP, 14801-903 Araraquara, BrazilExperimental Dento-Maxillo-Facial Medicine, Center of Dento-Maxillo-Facial Medicine, University of Bonn, 53111 Bonn, GermanyPeriodontitis is an inflammatory disease caused by pathogenic microorganisms and characterized by the destruction of the periodontium. Obese individuals have an increased risk of periodontitis, and elevated circulating levels of adipokines, such as nicotinamide phosphoribosyltransferase (NAMPT), may be a pathomechanistic link between both diseases. The aim of this in vitro study was to examine the regulation of periodontal ligament (PDL) cells by NAMPT and its production under inflammatory and infectious conditions. NAMPT caused a significant upregulation of 9 genes and downregulation of 3 genes, as analyzed by microarray analysis. Eight of these genes could be confirmed by real-time PCR: NAMPT induced a significant upregulation of EGR1, MMP-1, SYT7, ITPKA, CCL2, NTM, IGF2BP3, and NRP1. NAMPT also increased significantly the MMP-1 and CCL2 protein synthesis. NAMPT was significantly induced by interleukin-1β and the periodontal microorganism P. gingivalis. NAMPT may contribute to periodontitis through upregulation of MMP-1 and CCL2 in PDL cells. Increased NAMPT levels, as found in obesity, may therefore represent a mechanism whereby obesity could confer an increased risk of periodontitis. Furthermore, microbial and inflammatory signals may enhance the NAMPT synthesis in PDL cells and thereby contribute to the increased gingival and serum levels of this adipokine, as found in periodontitis.http://dx.doi.org/10.1155/2013/437123 |
| spellingShingle | Marjan Nokhbehsaim Sigrun Eick Andressa Vilas Boas Nogueira Per Hoffmann Stefan Herms Holger Fröhlich Søren Jepsen Andreas Jäger Joni Augusto Cirelli James Deschner Stimulation of MMP-1 and CCL2 by NAMPT in PDL Cells Mediators of Inflammation |
| title | Stimulation of MMP-1 and CCL2 by NAMPT in PDL Cells |
| title_full | Stimulation of MMP-1 and CCL2 by NAMPT in PDL Cells |
| title_fullStr | Stimulation of MMP-1 and CCL2 by NAMPT in PDL Cells |
| title_full_unstemmed | Stimulation of MMP-1 and CCL2 by NAMPT in PDL Cells |
| title_short | Stimulation of MMP-1 and CCL2 by NAMPT in PDL Cells |
| title_sort | stimulation of mmp 1 and ccl2 by nampt in pdl cells |
| url | http://dx.doi.org/10.1155/2013/437123 |
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