Extended-Spectrum Beta-Lactamase- and Plasmidic AmpC-Producing <i>Enterobacterales</i> among the Faecal Samples in the Bulgarian Community

Extended-Spectrum Beta-Lactamase- and Plasmidic AmpC-Producing <i>Enterobacterales</i> among the Faecal Samples in the Bulgarian Community

The aim of the present work was to genetically characterise cefotaxime-resistant enterobacteria isolated from community carriers in Bulgaria. In total, 717 faecal samples from children and adults in five medical centres in Sofia, Pleven and Burgas were examined. Antimicrobial susceptibility was eval...

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Main Authors: Petya Stankova, Lyudmila Boyanova, Daniela Atanasova, Sashka Mihaylova, Mariya Sredkova, Raina Gergova, Kalina Mihova, Rumyana Markovska
Format: Article
Language:English
Published: MDPI AG 2024-08-01
Series:Microorganisms
Subjects:
faecal carriage
CTX-M
DHA
<i>Esherichia coli</i>
<i>Klebsiella pneumoniae</i>
Bulgaria
Online Access:https://www.mdpi.com/2076-2607/12/9/1777
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Summary:The aim of the present work was to genetically characterise cefotaxime-resistant enterobacteria isolated from community carriers in Bulgaria. In total, 717 faecal samples from children and adults in five medical centres in Sofia, Pleven and Burgas were examined. Antimicrobial susceptibility was evaluated by the disk diffusion method. <i>bla</i><sub>ESBL</sub> or plasmidic AmpC (pAmpC) genes were detected by PCR and sequencing. MLST and ERIC-PCR were used to detect clonal relatedness. Among the faecal samples, 140 cefotaxime-resistant enterobacteria were found. The most frequently detected species was <i>Escherichia coli</i> (77.9%, 109/140 samples), followed by <i>Klebsiella pneumoniae</i> (7.9%, 11/140). Among the isolates, <i>bla</i><sub>CTX-M-15</sub> (37.1%) was predominant, followed by <i>bla</i><sub>CTX-M-3</sub> (19.2%), <i>bla</i><sub>CTX-M-14</sub> (10%), and <i>bla</i><sub>CTX-M-27</sub> (4.3 %). Genes encoding pAmpC were observed in 11.4% (<i>bla</i><sub>DHA-1</sub>, 16/140) and in 1.4% (<i>bla</i><sub>CMY-2</sub>, 2/140). The frequency of ESBL and pAmpC producers among the subjects was 14.6% and 2.5%, respectively. No carbapenem-resistant isolates were found. Four main clonal complexes (CC131, CC10, CC38, and CC155) were detected among <i>E. coli</i> isolates. The most common type was ST131, phylogroup B2 (16.5%). The increased frequency of ESBL- and pAmpC-producing enterobacteria in the community is a prerequisite for treatment failures of the associated infections and a good background for further studies.
ISSN:2076-2607

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