The Feasibility of Using Near-Infrared Spectroscopy and Chemometrics for Untargeted Detection of Protein Adulteration in Yogurt: Removing Unwanted Variations in Pure Yogurt
Untargeted detection of protein adulteration in Chinese yogurt was performed using near-infrared (NIR) spectroscopy and chemometrics class modelling techniques. sixty yogurt samples were prepared with pure and fresh milk from local market, and 197 adulterated yogurt samples were prepared by blending...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2013-01-01
|
| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/201873 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832556810606739456 |
|---|---|
| author | Lu Xu Si-Min Yan Chen-Bo Cai Zhen-Ji Wang Xiao-Ping Yu |
| author_facet | Lu Xu Si-Min Yan Chen-Bo Cai Zhen-Ji Wang Xiao-Ping Yu |
| author_sort | Lu Xu |
| collection | DOAJ |
| description | Untargeted detection of protein adulteration in Chinese yogurt was performed using near-infrared (NIR) spectroscopy and chemometrics class modelling techniques. sixty yogurt samples were prepared with pure and fresh milk from local market, and 197 adulterated yogurt samples were prepared by blending the pure yogurt objects with different levels of edible gelatin, industrial gelatin, and soy protein powder, which have been frequently used for yogurt adulteration. A recently proposed one-class partial least squares (OCPLS) model was used to model the NIR spectra of pure yogurt objects and analyze those of future objects. To improve the raw spectra, orthogonal projection (OP) of raw spectra onto the spectrum of pure water and standard normal variate (SNV) transformation were used to remove unwanted spectral variations. The best model was obtained with OP preprocessing with sensitivity of 0.900 and specificity of 0.949. Moreover, adulterations of yogurt with 1% (w/w) edible gelatin, 2% (w/w) industrial gelatin, and 2% (w/w) soy protein powder can be safely detected by the proposed method. This study demonstrates the potential of combining NIR spectroscopy and OCPLS as an untargeted detection tool for protein adulteration in yogurt. |
| format | Article |
| id | doaj-art-3ffcc3456484474b8ac76151fb7fa0e2 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-3ffcc3456484474b8ac76151fb7fa0e22025-02-03T05:44:16ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732013-01-01201310.1155/2013/201873201873The Feasibility of Using Near-Infrared Spectroscopy and Chemometrics for Untargeted Detection of Protein Adulteration in Yogurt: Removing Unwanted Variations in Pure YogurtLu Xu0Si-Min Yan1Chen-Bo Cai2Zhen-Ji Wang3Xiao-Ping Yu4Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Xueyuan Street, Xiasha Higher Education District, Hangzhou 310018, ChinaZhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Xueyuan Street, Xiasha Higher Education District, Hangzhou 310018, ChinaDepartment of Chemistry and Life Science, Chuxiong Normal University, Luchengnan Road, Chuxiong 675000, ChinaDepartment of Chemistry and Life Science, Chuxiong Normal University, Luchengnan Road, Chuxiong 675000, ChinaZhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Xueyuan Street, Xiasha Higher Education District, Hangzhou 310018, ChinaUntargeted detection of protein adulteration in Chinese yogurt was performed using near-infrared (NIR) spectroscopy and chemometrics class modelling techniques. sixty yogurt samples were prepared with pure and fresh milk from local market, and 197 adulterated yogurt samples were prepared by blending the pure yogurt objects with different levels of edible gelatin, industrial gelatin, and soy protein powder, which have been frequently used for yogurt adulteration. A recently proposed one-class partial least squares (OCPLS) model was used to model the NIR spectra of pure yogurt objects and analyze those of future objects. To improve the raw spectra, orthogonal projection (OP) of raw spectra onto the spectrum of pure water and standard normal variate (SNV) transformation were used to remove unwanted spectral variations. The best model was obtained with OP preprocessing with sensitivity of 0.900 and specificity of 0.949. Moreover, adulterations of yogurt with 1% (w/w) edible gelatin, 2% (w/w) industrial gelatin, and 2% (w/w) soy protein powder can be safely detected by the proposed method. This study demonstrates the potential of combining NIR spectroscopy and OCPLS as an untargeted detection tool for protein adulteration in yogurt.http://dx.doi.org/10.1155/2013/201873 |
| spellingShingle | Lu Xu Si-Min Yan Chen-Bo Cai Zhen-Ji Wang Xiao-Ping Yu The Feasibility of Using Near-Infrared Spectroscopy and Chemometrics for Untargeted Detection of Protein Adulteration in Yogurt: Removing Unwanted Variations in Pure Yogurt Journal of Analytical Methods in Chemistry |
| title | The Feasibility of Using Near-Infrared Spectroscopy and Chemometrics for Untargeted Detection of Protein Adulteration in Yogurt: Removing Unwanted Variations in Pure Yogurt |
| title_full | The Feasibility of Using Near-Infrared Spectroscopy and Chemometrics for Untargeted Detection of Protein Adulteration in Yogurt: Removing Unwanted Variations in Pure Yogurt |
| title_fullStr | The Feasibility of Using Near-Infrared Spectroscopy and Chemometrics for Untargeted Detection of Protein Adulteration in Yogurt: Removing Unwanted Variations in Pure Yogurt |
| title_full_unstemmed | The Feasibility of Using Near-Infrared Spectroscopy and Chemometrics for Untargeted Detection of Protein Adulteration in Yogurt: Removing Unwanted Variations in Pure Yogurt |
| title_short | The Feasibility of Using Near-Infrared Spectroscopy and Chemometrics for Untargeted Detection of Protein Adulteration in Yogurt: Removing Unwanted Variations in Pure Yogurt |
| title_sort | feasibility of using near infrared spectroscopy and chemometrics for untargeted detection of protein adulteration in yogurt removing unwanted variations in pure yogurt |
| url | http://dx.doi.org/10.1155/2013/201873 |
| work_keys_str_mv | AT luxu thefeasibilityofusingnearinfraredspectroscopyandchemometricsforuntargeteddetectionofproteinadulterationinyogurtremovingunwantedvariationsinpureyogurt AT siminyan thefeasibilityofusingnearinfraredspectroscopyandchemometricsforuntargeteddetectionofproteinadulterationinyogurtremovingunwantedvariationsinpureyogurt AT chenbocai thefeasibilityofusingnearinfraredspectroscopyandchemometricsforuntargeteddetectionofproteinadulterationinyogurtremovingunwantedvariationsinpureyogurt AT zhenjiwang thefeasibilityofusingnearinfraredspectroscopyandchemometricsforuntargeteddetectionofproteinadulterationinyogurtremovingunwantedvariationsinpureyogurt AT xiaopingyu thefeasibilityofusingnearinfraredspectroscopyandchemometricsforuntargeteddetectionofproteinadulterationinyogurtremovingunwantedvariationsinpureyogurt AT luxu feasibilityofusingnearinfraredspectroscopyandchemometricsforuntargeteddetectionofproteinadulterationinyogurtremovingunwantedvariationsinpureyogurt AT siminyan feasibilityofusingnearinfraredspectroscopyandchemometricsforuntargeteddetectionofproteinadulterationinyogurtremovingunwantedvariationsinpureyogurt AT chenbocai feasibilityofusingnearinfraredspectroscopyandchemometricsforuntargeteddetectionofproteinadulterationinyogurtremovingunwantedvariationsinpureyogurt AT zhenjiwang feasibilityofusingnearinfraredspectroscopyandchemometricsforuntargeteddetectionofproteinadulterationinyogurtremovingunwantedvariationsinpureyogurt AT xiaopingyu feasibilityofusingnearinfraredspectroscopyandchemometricsforuntargeteddetectionofproteinadulterationinyogurtremovingunwantedvariationsinpureyogurt |