Role of CD117 and mast cells in periodontal diseases: Histological and immunohistochemical study
Mast cells are mobile secretory cells with a variety of biological tasks such as phagocytosis, processing of antigens, cytokine production, and the release of both newly formed and preexisting physiological mediators. This study aimed to investigate and establish the relationship between mast cells...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology
2025-03-01
|
| Series: | Journal of Advanced Biotechnology and Experimental Therapeutics |
| Subjects: | |
| Online Access: | https://www.bsmiab.org/jabet/?mno=218355 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832578888525414400 |
|---|---|
| author | Fatin L. Khaphi Hanaa Ali Hussein Oula Fouad Hameed Huda Jassem Jebur Zahra Kadhum Saeed |
| author_facet | Fatin L. Khaphi Hanaa Ali Hussein Oula Fouad Hameed Huda Jassem Jebur Zahra Kadhum Saeed |
| author_sort | Fatin L. Khaphi |
| collection | DOAJ |
| description | Mast cells are mobile secretory cells with a variety of biological tasks such as phagocytosis, processing of antigens, cytokine production, and the release of both newly formed and preexisting physiological mediators. This study aimed to investigate and establish the relationship between mast cells and various stages of periodontal diseases. A total of 36 soft tissue samples, 12 each of normal healthy periodontal, dental plaque periodontitis, and chronic periodontitis were selected. Samples were collected from patients with periodontal surgery in chronic periodontitis and plaque periodontitis. Samples were stained with 1% toluidine blue (TB), immunohistochemistry for CD117 expression, and observed under a light microscope for the presence of mast cells. All data were analyzed using SPSS. The mast cells were found to be highest in the chronic periodontitis group, followed by the dental plaque periodontitis group, as compared to the healthy periodontal group. The quantitative study of mast cells using TB staining showed statistically significant differences among the three groups. However, the results showed no statistically significant difference in the expression levels of CD117 in the plaque periodontitis and healthy periodontal group but showed a statistically significant difference between chronic periodontitis and healthy periodontal group. Thus, an increase in mast cells in human periodontal diseases suggests that these cells are crucial for developing chronic periodontitis. [ J Adv Biotechnol Exp Ther 2025; 8(1.000): 182-191] |
| format | Article |
| id | doaj-art-3fe34f347d9643518f1d4712fbd91820 |
| institution | Kabale University |
| issn | 2616-4760 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology |
| record_format | Article |
| series | Journal of Advanced Biotechnology and Experimental Therapeutics |
| spelling | doaj-art-3fe34f347d9643518f1d4712fbd918202025-01-30T13:01:08ZengBangladesh Society for Microbiology, Immunology, and Advanced BiotechnologyJournal of Advanced Biotechnology and Experimental Therapeutics2616-47602025-03-018118219110.5455/jabet.2025.15218355Role of CD117 and mast cells in periodontal diseases: Histological and immunohistochemical studyFatin L. Khaphi0Hanaa Ali Hussein1Oula Fouad Hameed2Huda Jassem Jebur3Zahra Kadhum Saeed4University of Basrah, College of Dentistry, Basic Science Branch University of Basrah, College of Dentistry, Basic Science Branch University of Basrah, College of Dentistry, Basic Science Branch University of Basrah, College of Dentistry University of Basrah, College of Dentistry, Basic Science BranchMast cells are mobile secretory cells with a variety of biological tasks such as phagocytosis, processing of antigens, cytokine production, and the release of both newly formed and preexisting physiological mediators. This study aimed to investigate and establish the relationship between mast cells and various stages of periodontal diseases. A total of 36 soft tissue samples, 12 each of normal healthy periodontal, dental plaque periodontitis, and chronic periodontitis were selected. Samples were collected from patients with periodontal surgery in chronic periodontitis and plaque periodontitis. Samples were stained with 1% toluidine blue (TB), immunohistochemistry for CD117 expression, and observed under a light microscope for the presence of mast cells. All data were analyzed using SPSS. The mast cells were found to be highest in the chronic periodontitis group, followed by the dental plaque periodontitis group, as compared to the healthy periodontal group. The quantitative study of mast cells using TB staining showed statistically significant differences among the three groups. However, the results showed no statistically significant difference in the expression levels of CD117 in the plaque periodontitis and healthy periodontal group but showed a statistically significant difference between chronic periodontitis and healthy periodontal group. Thus, an increase in mast cells in human periodontal diseases suggests that these cells are crucial for developing chronic periodontitis. [ J Adv Biotechnol Exp Ther 2025; 8(1.000): 182-191]https://www.bsmiab.org/jabet/?mno=218355mast cellplaque periodontitischronic periodontitistoluidine blue (tb)cd117 |
| spellingShingle | Fatin L. Khaphi Hanaa Ali Hussein Oula Fouad Hameed Huda Jassem Jebur Zahra Kadhum Saeed Role of CD117 and mast cells in periodontal diseases: Histological and immunohistochemical study Journal of Advanced Biotechnology and Experimental Therapeutics mast cell plaque periodontitis chronic periodontitis toluidine blue (tb) cd117 |
| title | Role of CD117 and mast cells in periodontal diseases: Histological and immunohistochemical study |
| title_full | Role of CD117 and mast cells in periodontal diseases: Histological and immunohistochemical study |
| title_fullStr | Role of CD117 and mast cells in periodontal diseases: Histological and immunohistochemical study |
| title_full_unstemmed | Role of CD117 and mast cells in periodontal diseases: Histological and immunohistochemical study |
| title_short | Role of CD117 and mast cells in periodontal diseases: Histological and immunohistochemical study |
| title_sort | role of cd117 and mast cells in periodontal diseases histological and immunohistochemical study |
| topic | mast cell plaque periodontitis chronic periodontitis toluidine blue (tb) cd117 |
| url | https://www.bsmiab.org/jabet/?mno=218355 |
| work_keys_str_mv | AT fatinlkhaphi roleofcd117andmastcellsinperiodontaldiseaseshistologicalandimmunohistochemicalstudy AT hanaaalihussein roleofcd117andmastcellsinperiodontaldiseaseshistologicalandimmunohistochemicalstudy AT oulafouadhameed roleofcd117andmastcellsinperiodontaldiseaseshistologicalandimmunohistochemicalstudy AT hudajassemjebur roleofcd117andmastcellsinperiodontaldiseaseshistologicalandimmunohistochemicalstudy AT zahrakadhumsaeed roleofcd117andmastcellsinperiodontaldiseaseshistologicalandimmunohistochemicalstudy |