Electroacupuncture alleviates damage to myopic RGCs probably through lncRNA-XR_002789763.1-mediated mitophagy
Abstract Background Mitophagy is closely related to the regulation of retinal ganglion cell (RGC) structure and function. Our previous study suggested that long noncoding RNAs (lncRNAs) can cause damage to myopic RGCs. However, whether electroacupuncture (EA) treatment can delay myopia progression t...
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BMC
2025-02-01
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| Series: | Chinese Medicine |
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| Online Access: | https://doi.org/10.1186/s13020-025-01058-5 |
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| author | Xuejun Wang Qinghong Lin Li Tian Xiaoying Li Teruko Fukuyama Weijung Ten Xiehe Kong Yanting Yang Xiaopeng Ma Xingtao Zhou |
| author_facet | Xuejun Wang Qinghong Lin Li Tian Xiaoying Li Teruko Fukuyama Weijung Ten Xiehe Kong Yanting Yang Xiaopeng Ma Xingtao Zhou |
| author_sort | Xuejun Wang |
| collection | DOAJ |
| description | Abstract Background Mitophagy is closely related to the regulation of retinal ganglion cell (RGC) structure and function. Our previous study suggested that long noncoding RNAs (lncRNAs) can cause damage to myopic RGCs. However, whether electroacupuncture (EA) treatment can delay myopia progression through lncRNA-mediated mitophagy in RGCs is currently unknown. This study aimed to investigate the effect of EA on lncRNA-mediated mitophagy in myopic RGCs. Methods Our study investigated the modulatory effect of EA on mitophagy in RGCs of guinea pigs with form-deprived myopia (FDM). RNA sequencing was performed to further analyze the expression profiles of lncRNAs and mRNAs in RGCs of guinea pigs with FDM after EA treatment, and the related competing endogenous RNA (ceRNA) network was constructed. Importantly, PINK1, a mitophagy-related gene, was included in the core ceRNA network to explore the relationship between lncRNAs and mitophagy in myopic RGCs regulated by EA. We also collected eyeballs from myopic and highly myopic adults to further verify the mechanistic results. Results This study demonstrated that EA treatment delayed the reduction in refraction and increase in axial length and alleviated RGC damage in guinea pigs with FDM. We further found that EA could induce mitophagy in guinea pig RGCs with FDM by promoting the mitophagy-related PINK1/Parkin signaling pathway. Moreover, mitophagy is inhibited in the retina of highly myopic adults. RNA sequencing revealed that 599 lncRNAs and 455 mRNAs were differentially expressed in guinea pig RGCs with FDM after EA treatment. A core ceRNA network was constructed by incorporating PINK1 and verified by related molecular experiments, and we found that EA treatment may induce mitophagy and attenuated RGC injury in guinea pigs with FDM by sponging miR-342-5p through lncRNA-XR_002789763.1 to activate the PINK1/Parkin signaling pathway and promote Mfn2 ubiquitination. Conclusion EA treatment might regulate lncRNA-XR_002789763.1/miR-342-5p axis and activate the mitophagy-related PINK1/Parkin signaling pathway, and promote Mfn2 ubiquitination, thereby alleviating RGC damage and delaying myopia progression. |
| format | Article |
| id | doaj-art-3fdcf990e6454ed996eec4742ebd7e46 |
| institution | Kabale University |
| issn | 1749-8546 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
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| series | Chinese Medicine |
| spelling | doaj-art-3fdcf990e6454ed996eec4742ebd7e462025-02-02T12:42:36ZengBMCChinese Medicine1749-85462025-02-0120112110.1186/s13020-025-01058-5Electroacupuncture alleviates damage to myopic RGCs probably through lncRNA-XR_002789763.1-mediated mitophagyXuejun Wang0Qinghong Lin1Li Tian2Xiaoying Li3Teruko Fukuyama4Weijung Ten5Xiehe Kong6Yanting Yang7Xiaopeng Ma8Xingtao Zhou9Eye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan UniversityEye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan UniversityLonghua Hospital, Shanghai University of Traditional Chinese MedicineYueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese MedicineEye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan UniversityEye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan UniversityYueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese MedicineYueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese MedicineYueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese MedicineEye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan UniversityAbstract Background Mitophagy is closely related to the regulation of retinal ganglion cell (RGC) structure and function. Our previous study suggested that long noncoding RNAs (lncRNAs) can cause damage to myopic RGCs. However, whether electroacupuncture (EA) treatment can delay myopia progression through lncRNA-mediated mitophagy in RGCs is currently unknown. This study aimed to investigate the effect of EA on lncRNA-mediated mitophagy in myopic RGCs. Methods Our study investigated the modulatory effect of EA on mitophagy in RGCs of guinea pigs with form-deprived myopia (FDM). RNA sequencing was performed to further analyze the expression profiles of lncRNAs and mRNAs in RGCs of guinea pigs with FDM after EA treatment, and the related competing endogenous RNA (ceRNA) network was constructed. Importantly, PINK1, a mitophagy-related gene, was included in the core ceRNA network to explore the relationship between lncRNAs and mitophagy in myopic RGCs regulated by EA. We also collected eyeballs from myopic and highly myopic adults to further verify the mechanistic results. Results This study demonstrated that EA treatment delayed the reduction in refraction and increase in axial length and alleviated RGC damage in guinea pigs with FDM. We further found that EA could induce mitophagy in guinea pig RGCs with FDM by promoting the mitophagy-related PINK1/Parkin signaling pathway. Moreover, mitophagy is inhibited in the retina of highly myopic adults. RNA sequencing revealed that 599 lncRNAs and 455 mRNAs were differentially expressed in guinea pig RGCs with FDM after EA treatment. A core ceRNA network was constructed by incorporating PINK1 and verified by related molecular experiments, and we found that EA treatment may induce mitophagy and attenuated RGC injury in guinea pigs with FDM by sponging miR-342-5p through lncRNA-XR_002789763.1 to activate the PINK1/Parkin signaling pathway and promote Mfn2 ubiquitination. Conclusion EA treatment might regulate lncRNA-XR_002789763.1/miR-342-5p axis and activate the mitophagy-related PINK1/Parkin signaling pathway, and promote Mfn2 ubiquitination, thereby alleviating RGC damage and delaying myopia progression.https://doi.org/10.1186/s13020-025-01058-5MyopiaMitophagyLncRNARetinal ganglion cellLncRNA-XR_002789763.1/miR-342-5p/PINK1 axis |
| spellingShingle | Xuejun Wang Qinghong Lin Li Tian Xiaoying Li Teruko Fukuyama Weijung Ten Xiehe Kong Yanting Yang Xiaopeng Ma Xingtao Zhou Electroacupuncture alleviates damage to myopic RGCs probably through lncRNA-XR_002789763.1-mediated mitophagy Chinese Medicine Myopia Mitophagy LncRNA Retinal ganglion cell LncRNA-XR_002789763.1/miR-342-5p/PINK1 axis |
| title | Electroacupuncture alleviates damage to myopic RGCs probably through lncRNA-XR_002789763.1-mediated mitophagy |
| title_full | Electroacupuncture alleviates damage to myopic RGCs probably through lncRNA-XR_002789763.1-mediated mitophagy |
| title_fullStr | Electroacupuncture alleviates damage to myopic RGCs probably through lncRNA-XR_002789763.1-mediated mitophagy |
| title_full_unstemmed | Electroacupuncture alleviates damage to myopic RGCs probably through lncRNA-XR_002789763.1-mediated mitophagy |
| title_short | Electroacupuncture alleviates damage to myopic RGCs probably through lncRNA-XR_002789763.1-mediated mitophagy |
| title_sort | electroacupuncture alleviates damage to myopic rgcs probably through lncrna xr 002789763 1 mediated mitophagy |
| topic | Myopia Mitophagy LncRNA Retinal ganglion cell LncRNA-XR_002789763.1/miR-342-5p/PINK1 axis |
| url | https://doi.org/10.1186/s13020-025-01058-5 |
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