The Rise of CRISPR/Cas for Genome Editing in Stem Cells
Genetic manipulation is a powerful tool to establish the causal relationship between a genetic lesion and a particular pathological phenotype. The rise of CRISPR/Cas9 genome-engineering tools overcame the traditional technical bottleneck for routine site-specific genetic manipulation in cells. To cr...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/8140168 |
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| _version_ | 1832562316185436160 |
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| author | Bing Shui Liz Hernandez Matias Yi Guo Ying Peng |
| author_facet | Bing Shui Liz Hernandez Matias Yi Guo Ying Peng |
| author_sort | Bing Shui |
| collection | DOAJ |
| description | Genetic manipulation is a powerful tool to establish the causal relationship between a genetic lesion and a particular pathological phenotype. The rise of CRISPR/Cas9 genome-engineering tools overcame the traditional technical bottleneck for routine site-specific genetic manipulation in cells. To create the perfect in vitro cell model, there is significant interest from the stem cell research community to adopt this fast evolving technology. This review addresses this need directly by providing both the up-to-date biochemical rationale of CRISPR-mediated genome engineering and detailed practical guidelines for the design and execution of CRISPR experiments in cell models. Ultimately, this review will serve as a timely and comprehensive guide for this fast developing technology. |
| format | Article |
| id | doaj-art-3fdb7d42d946439d8881a76a41e72ff3 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-3fdb7d42d946439d8881a76a41e72ff32025-02-03T01:22:55ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/81401688140168The Rise of CRISPR/Cas for Genome Editing in Stem CellsBing Shui0Liz Hernandez Matias1Yi Guo2Ying Peng3Department of Biology, Carleton College, Northfield, MN 55057, USADepartment of Biology, University of Puerto Rico, Rio Piedras, San Juan, PR 00931, USADepartment of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USADepartment of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USAGenetic manipulation is a powerful tool to establish the causal relationship between a genetic lesion and a particular pathological phenotype. The rise of CRISPR/Cas9 genome-engineering tools overcame the traditional technical bottleneck for routine site-specific genetic manipulation in cells. To create the perfect in vitro cell model, there is significant interest from the stem cell research community to adopt this fast evolving technology. This review addresses this need directly by providing both the up-to-date biochemical rationale of CRISPR-mediated genome engineering and detailed practical guidelines for the design and execution of CRISPR experiments in cell models. Ultimately, this review will serve as a timely and comprehensive guide for this fast developing technology.http://dx.doi.org/10.1155/2016/8140168 |
| spellingShingle | Bing Shui Liz Hernandez Matias Yi Guo Ying Peng The Rise of CRISPR/Cas for Genome Editing in Stem Cells Stem Cells International |
| title | The Rise of CRISPR/Cas for Genome Editing in Stem Cells |
| title_full | The Rise of CRISPR/Cas for Genome Editing in Stem Cells |
| title_fullStr | The Rise of CRISPR/Cas for Genome Editing in Stem Cells |
| title_full_unstemmed | The Rise of CRISPR/Cas for Genome Editing in Stem Cells |
| title_short | The Rise of CRISPR/Cas for Genome Editing in Stem Cells |
| title_sort | rise of crispr cas for genome editing in stem cells |
| url | http://dx.doi.org/10.1155/2016/8140168 |
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