Preparation of Nanoparticle-Immobilized Gold Surfaces for the Reversible Conjugation of Neurotensin Peptide
Polymer coatings as thin films stand out as a commonly used strategy to modify biosensor surfaces for improving detection performance; however, nonspecific biomolecule interactions and the limited degree of ligand conjugation on the surface have necessitated the development of innovative methods for...
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MDPI AG
2025-05-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/15/6/767 |
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| author | Hidayet Gok Deniz Gol Betul Zehra Temur Nureddin Turkan Ozge Can Ceyhun Ekrem Kirimli Gokcen Ozgun Ozgul Gok |
| author_facet | Hidayet Gok Deniz Gol Betul Zehra Temur Nureddin Turkan Ozge Can Ceyhun Ekrem Kirimli Gokcen Ozgun Ozgul Gok |
| author_sort | Hidayet Gok |
| collection | DOAJ |
| description | Polymer coatings as thin films stand out as a commonly used strategy to modify biosensor surfaces for improving detection performance; however, nonspecific biomolecule interactions and the limited degree of ligand conjugation on the surface have necessitated the development of innovative methods for surface modification. To this end, methacrylated tethered telechelic polyethylene glycol (PEG-diMA) chains of three different molecular weights (2, 6, and 10 kDa) were synthesized herein and used for obtaining thiolated nanoparticles (NPs) upon adding excess amounts of a tetra-thiol crosslinker. Characterized according to their size, surface charge, morphology, and thiol amounts, these nanoparticles were immobilized on gold surfaces that mimicked gold-coated mass sensor platforms. The PEG-based nanoparticles, prepared especially by PEG6K-diMA polymers, were shown to result in the preparation of a monolayer and smooth coating of 80–120 nm thickness. Cysteine-modified NTS(8–13) peptide (RRPYIL) was conjugated to thiolated NP with reversible disulfide bonds and it was demonstrated that its cleavage with a reducing agent such as dithiothreitol (DTT) restores the NP-immobilized gold surface for at least two cycles. Together with its binding studies to NTSR2 antibodies, it was revealed that the peptide-conjugated NP-modified gold surface could be employed as a model for a reusable sensor surface for the detection of biomarkers of same or different types. |
| format | Article |
| id | doaj-art-3f907b091cd340f7b44b2b563fdfdae7 |
| institution | Kabale University |
| issn | 2218-273X |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
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| series | Biomolecules |
| spelling | doaj-art-3f907b091cd340f7b44b2b563fdfdae72025-08-20T03:26:20ZengMDPI AGBiomolecules2218-273X2025-05-0115676710.3390/biom15060767Preparation of Nanoparticle-Immobilized Gold Surfaces for the Reversible Conjugation of Neurotensin PeptideHidayet Gok0Deniz Gol1Betul Zehra Temur2Nureddin Turkan3Ozge Can4Ceyhun Ekrem Kirimli5Gokcen Ozgun6Ozgul Gok7Department of Nanoscience and Nanoengineering, School of Engineering and Natural Sciences, Istanbul Medeniyet University, 34720 Istanbul, TurkeyDepartment of Biomedical Engineering, Institute of Natural and Applied Sciences, Acibadem Mehmet Ali Aydinlar University, 34752 Istanbul, TurkeyDepartment of Medical Biotechnology, Institute of Health Sciences, Acibadem Mehmet Ali Aydinlar University, 34752 Istanbul, TurkeyDepartment of Physics Engineering, Faculty of Engineering and Natural Sciences, Istanbul Medeniyet University, 34720 Istanbul, TurkeyDepartment of Biomedical Engineering, Institute of Natural and Applied Sciences, Acibadem Mehmet Ali Aydinlar University, 34752 Istanbul, TurkeyDepartment of Biomedical Engineering, Institute of Natural and Applied Sciences, Acibadem Mehmet Ali Aydinlar University, 34752 Istanbul, TurkeyDepartment of Medical Biotechnology, Institute of Health Sciences, Acibadem University, 34752 Istanbul, TurkeyDepartment of Biomedical Engineering, Institute of Natural and Applied Sciences, Acibadem Mehmet Ali Aydinlar University, 34752 Istanbul, TurkeyPolymer coatings as thin films stand out as a commonly used strategy to modify biosensor surfaces for improving detection performance; however, nonspecific biomolecule interactions and the limited degree of ligand conjugation on the surface have necessitated the development of innovative methods for surface modification. To this end, methacrylated tethered telechelic polyethylene glycol (PEG-diMA) chains of three different molecular weights (2, 6, and 10 kDa) were synthesized herein and used for obtaining thiolated nanoparticles (NPs) upon adding excess amounts of a tetra-thiol crosslinker. Characterized according to their size, surface charge, morphology, and thiol amounts, these nanoparticles were immobilized on gold surfaces that mimicked gold-coated mass sensor platforms. The PEG-based nanoparticles, prepared especially by PEG6K-diMA polymers, were shown to result in the preparation of a monolayer and smooth coating of 80–120 nm thickness. Cysteine-modified NTS(8–13) peptide (RRPYIL) was conjugated to thiolated NP with reversible disulfide bonds and it was demonstrated that its cleavage with a reducing agent such as dithiothreitol (DTT) restores the NP-immobilized gold surface for at least two cycles. Together with its binding studies to NTSR2 antibodies, it was revealed that the peptide-conjugated NP-modified gold surface could be employed as a model for a reusable sensor surface for the detection of biomarkers of same or different types.https://www.mdpi.com/2218-273X/15/6/767reactive nanoparticlespoly(ethylene glycol)surface modificationcoatingdisulfide exchange reactionneurotensin |
| spellingShingle | Hidayet Gok Deniz Gol Betul Zehra Temur Nureddin Turkan Ozge Can Ceyhun Ekrem Kirimli Gokcen Ozgun Ozgul Gok Preparation of Nanoparticle-Immobilized Gold Surfaces for the Reversible Conjugation of Neurotensin Peptide Biomolecules reactive nanoparticles poly(ethylene glycol) surface modification coating disulfide exchange reaction neurotensin |
| title | Preparation of Nanoparticle-Immobilized Gold Surfaces for the Reversible Conjugation of Neurotensin Peptide |
| title_full | Preparation of Nanoparticle-Immobilized Gold Surfaces for the Reversible Conjugation of Neurotensin Peptide |
| title_fullStr | Preparation of Nanoparticle-Immobilized Gold Surfaces for the Reversible Conjugation of Neurotensin Peptide |
| title_full_unstemmed | Preparation of Nanoparticle-Immobilized Gold Surfaces for the Reversible Conjugation of Neurotensin Peptide |
| title_short | Preparation of Nanoparticle-Immobilized Gold Surfaces for the Reversible Conjugation of Neurotensin Peptide |
| title_sort | preparation of nanoparticle immobilized gold surfaces for the reversible conjugation of neurotensin peptide |
| topic | reactive nanoparticles poly(ethylene glycol) surface modification coating disulfide exchange reaction neurotensin |
| url | https://www.mdpi.com/2218-273X/15/6/767 |
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