Screening and evaluation of Biofilm forming antibiotic-resistant bacterial strains isolated from wastewater treatment plants and river environment
This study aimed to assess the antibiotic resistance and biofilm formation efficiency in different bacterial strains isolated from wastewater treatment plants (WWTPs) and Ganges River environments of three North Indian cities in summer and winter. A total of 104 morphologically distinct bacterial s...
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Action for Sustainable Efficacious Development and Awareness
2025-03-01
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| Series: | Environment Conservation Journal |
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| Online Access: | https://journal.environcj.in/index.php/ecj/article/view/2956 |
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| author | Arun Kumar Pal Pooja Tripathi Vijay Tripathi |
| author_facet | Arun Kumar Pal Pooja Tripathi Vijay Tripathi |
| author_sort | Arun Kumar Pal |
| collection | DOAJ |
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This study aimed to assess the antibiotic resistance and biofilm formation efficiency in different bacterial strains isolated from wastewater treatment plants (WWTPs) and Ganges River environments of three North Indian cities in summer and winter. A total of 104 morphologically distinct bacterial strains were isolated from wastewater and river samples and molecularly characterized by 16S rRNA gene sequencing. All bacterial strains showed multidrug resistance against the most commonly used antibiotics. The biofilm formation ability of bacterial strains was evaluated through the crystal violet method. According to the results, 54.38% of bacterial isolates were strong biofilm formers isolated in the summer season, whereas 35.41% isolated in the winter were strong biofilm formers after 72 hours of incubation. Among the biofilm-forming bacterial isolates, the highest autoaggregation index was observed in Kerstersia gyiorum (summer) and Bacillus cereus (winter). However, some bacterial strains capable of biofilm formation showed no aggregation properties. Thus, the present study showed that multidrug-resistant bacterial strains isolated from wastewater and river environments can form biofilm. Also, this study suggested the development of innovative bioinocula in antibiotic degradation and preventing biofilm development.
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| format | Article |
| id | doaj-art-3f886e92dcf44cd5bb9f4eef2b110c31 |
| institution | Kabale University |
| issn | 0972-3099 2278-5124 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Action for Sustainable Efficacious Development and Awareness |
| record_format | Article |
| series | Environment Conservation Journal |
| spelling | doaj-art-3f886e92dcf44cd5bb9f4eef2b110c312025-08-20T03:47:16ZengAction for Sustainable Efficacious Development and AwarenessEnvironment Conservation Journal0972-30992278-51242025-03-0126210.36953/ECJ.29562934Screening and evaluation of Biofilm forming antibiotic-resistant bacterial strains isolated from wastewater treatment plants and river environmentArun Kumar Pal0Pooja Tripathi1Vijay Tripathi2Department of Molecular and Cellular Engineering, Jacob Institute of Biotechnology and Bioengineering, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, U.P, IndiaDepartment of Computational Biology and Bioinformatics, Jacob Institute of Biotechnology and Bioengineering,Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, U.P, IndiaDepartment of Microbiology Graphic Era deemed to be University Clement Town, Dehradun, India This study aimed to assess the antibiotic resistance and biofilm formation efficiency in different bacterial strains isolated from wastewater treatment plants (WWTPs) and Ganges River environments of three North Indian cities in summer and winter. A total of 104 morphologically distinct bacterial strains were isolated from wastewater and river samples and molecularly characterized by 16S rRNA gene sequencing. All bacterial strains showed multidrug resistance against the most commonly used antibiotics. The biofilm formation ability of bacterial strains was evaluated through the crystal violet method. According to the results, 54.38% of bacterial isolates were strong biofilm formers isolated in the summer season, whereas 35.41% isolated in the winter were strong biofilm formers after 72 hours of incubation. Among the biofilm-forming bacterial isolates, the highest autoaggregation index was observed in Kerstersia gyiorum (summer) and Bacillus cereus (winter). However, some bacterial strains capable of biofilm formation showed no aggregation properties. Thus, the present study showed that multidrug-resistant bacterial strains isolated from wastewater and river environments can form biofilm. Also, this study suggested the development of innovative bioinocula in antibiotic degradation and preventing biofilm development. https://journal.environcj.in/index.php/ecj/article/view/2956AntibioticAntibiotic resistanceBiofilmMultidrug resistancewastewater treatment |
| spellingShingle | Arun Kumar Pal Pooja Tripathi Vijay Tripathi Screening and evaluation of Biofilm forming antibiotic-resistant bacterial strains isolated from wastewater treatment plants and river environment Environment Conservation Journal Antibiotic Antibiotic resistance Biofilm Multidrug resistance wastewater treatment |
| title | Screening and evaluation of Biofilm forming antibiotic-resistant bacterial strains isolated from wastewater treatment plants and river environment |
| title_full | Screening and evaluation of Biofilm forming antibiotic-resistant bacterial strains isolated from wastewater treatment plants and river environment |
| title_fullStr | Screening and evaluation of Biofilm forming antibiotic-resistant bacterial strains isolated from wastewater treatment plants and river environment |
| title_full_unstemmed | Screening and evaluation of Biofilm forming antibiotic-resistant bacterial strains isolated from wastewater treatment plants and river environment |
| title_short | Screening and evaluation of Biofilm forming antibiotic-resistant bacterial strains isolated from wastewater treatment plants and river environment |
| title_sort | screening and evaluation of biofilm forming antibiotic resistant bacterial strains isolated from wastewater treatment plants and river environment |
| topic | Antibiotic Antibiotic resistance Biofilm Multidrug resistance wastewater treatment |
| url | https://journal.environcj.in/index.php/ecj/article/view/2956 |
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