Evaluation of Epstein-Barr Virus Latent Membrane Protein 2 Specific T-Cell Receptors Driven by T-Cell Specific Promoters Using Lentiviral Vector
Transduction of latent membrane protein 2 (LMP2)-specific T-cell receptors into activated T lymphocytes may provide a universal, MHC-restricted mean to treat EBV-associated tumors in adoptive immunotherapy. We compared TCR-specific promoters of distinct origin in lentiviral vectors, that is, Vβ6.7,...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2011/716926 |
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| author | Dongchang Yang Qing Shao Hua Sun Xiaoxin Mu Yun Gao Runqiu Jiang Jiajie Hou Kun Yao Yun Chen Beicheng Sun |
| author_facet | Dongchang Yang Qing Shao Hua Sun Xiaoxin Mu Yun Gao Runqiu Jiang Jiajie Hou Kun Yao Yun Chen Beicheng Sun |
| author_sort | Dongchang Yang |
| collection | DOAJ |
| description | Transduction of latent membrane protein 2 (LMP2)-specific T-cell receptors into activated T lymphocytes may provide a universal, MHC-restricted mean to treat EBV-associated tumors in adoptive immunotherapy. We compared TCR-specific promoters of distinct origin in lentiviral vectors, that is, Vβ6.7, delta, luria, and Vβ5.1 to evaluate TCR gene expression in human primary peripheral blood monocytes and T cell line HSB2. Vectors containing Vβ 6.7 promoter were found to be optimal for expression in PBMCs, and they maintained expression of the transduced TCRs for up to 7 weeks. These cells had the potential to recognize subdominant EBV latency antigens as measured by cytotoxicity and IFN-γ secretion. The nude mice also exhibited significant resistance to the HLA-A2 and LMP2-positive CNE tumor cell challenge after being infused with lentiviral transduced CTLs. In conclusion, LMP2-specific CTLs by lentiviral transduction have the potential use for treatment of EBV-related tumors. |
| format | Article |
| id | doaj-art-3f7914f3eb564f91b3d0cd0000709ab5 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-3f7914f3eb564f91b3d0cd0000709ab52025-02-03T06:14:14ZengWileyClinical and Developmental Immunology1740-25221740-25302011-01-01201110.1155/2011/716926716926Evaluation of Epstein-Barr Virus Latent Membrane Protein 2 Specific T-Cell Receptors Driven by T-Cell Specific Promoters Using Lentiviral VectorDongchang Yang0Qing Shao1Hua Sun2Xiaoxin Mu3Yun Gao4Runqiu Jiang5Jiajie Hou6Kun Yao7Yun Chen8Beicheng Sun9Liver Transplantation Center, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province, Nanjing 210029, ChinaDepartment of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province, Nanjing 210029, ChinaBrown Foundation Institute of Molecular Medicine, The University of Texas Health Science Center at Houston, Houston, TX 77030, USALiver Transplantation Center, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province, Nanjing 210029, ChinaLiver Transplantation Center, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province, Nanjing 210029, ChinaLiver Transplantation Center, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province, Nanjing 210029, ChinaLiver Transplantation Center, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province, Nanjing 210029, ChinaDepartment of Microbiology and Immunology, Nanjing Medical University, Nanjing 210029, ChinaLiver Transplantation Center, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province, Nanjing 210029, ChinaLiver Transplantation Center, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province, Nanjing 210029, ChinaTransduction of latent membrane protein 2 (LMP2)-specific T-cell receptors into activated T lymphocytes may provide a universal, MHC-restricted mean to treat EBV-associated tumors in adoptive immunotherapy. We compared TCR-specific promoters of distinct origin in lentiviral vectors, that is, Vβ6.7, delta, luria, and Vβ5.1 to evaluate TCR gene expression in human primary peripheral blood monocytes and T cell line HSB2. Vectors containing Vβ 6.7 promoter were found to be optimal for expression in PBMCs, and they maintained expression of the transduced TCRs for up to 7 weeks. These cells had the potential to recognize subdominant EBV latency antigens as measured by cytotoxicity and IFN-γ secretion. The nude mice also exhibited significant resistance to the HLA-A2 and LMP2-positive CNE tumor cell challenge after being infused with lentiviral transduced CTLs. In conclusion, LMP2-specific CTLs by lentiviral transduction have the potential use for treatment of EBV-related tumors.http://dx.doi.org/10.1155/2011/716926 |
| spellingShingle | Dongchang Yang Qing Shao Hua Sun Xiaoxin Mu Yun Gao Runqiu Jiang Jiajie Hou Kun Yao Yun Chen Beicheng Sun Evaluation of Epstein-Barr Virus Latent Membrane Protein 2 Specific T-Cell Receptors Driven by T-Cell Specific Promoters Using Lentiviral Vector Clinical and Developmental Immunology |
| title | Evaluation of Epstein-Barr Virus Latent Membrane Protein 2 Specific T-Cell Receptors Driven by T-Cell Specific Promoters Using Lentiviral Vector |
| title_full | Evaluation of Epstein-Barr Virus Latent Membrane Protein 2 Specific T-Cell Receptors Driven by T-Cell Specific Promoters Using Lentiviral Vector |
| title_fullStr | Evaluation of Epstein-Barr Virus Latent Membrane Protein 2 Specific T-Cell Receptors Driven by T-Cell Specific Promoters Using Lentiviral Vector |
| title_full_unstemmed | Evaluation of Epstein-Barr Virus Latent Membrane Protein 2 Specific T-Cell Receptors Driven by T-Cell Specific Promoters Using Lentiviral Vector |
| title_short | Evaluation of Epstein-Barr Virus Latent Membrane Protein 2 Specific T-Cell Receptors Driven by T-Cell Specific Promoters Using Lentiviral Vector |
| title_sort | evaluation of epstein barr virus latent membrane protein 2 specific t cell receptors driven by t cell specific promoters using lentiviral vector |
| url | http://dx.doi.org/10.1155/2011/716926 |
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