Sweat-Sensing Patches with Integrated Hydrogel Interface for Resting Sweat Collection and Multi-Information Detection

Sweat analysis represents an emerging non-invasive approach for health monitoring, yet its practical application is hindered by challenges such as insufficient natural sweat secretion and inefficient collection. To overcome these limitations, this study develops a hydrogel sheet composed of agarose...

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Main Authors: Lei Lu, Qiang Sun, Zihao Lin, Wenjie Xu, Xiangnan Li, Tian Wang, Yiming Lu, Huaping Wu, Lin Cheng, Aiping Liu
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Biosensors
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Online Access:https://www.mdpi.com/2079-6374/15/6/342
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Summary:Sweat analysis represents an emerging non-invasive approach for health monitoring, yet its practical application is hindered by challenges such as insufficient natural sweat secretion and inefficient collection. To overcome these limitations, this study develops a hydrogel sheet composed of agarose and glycerol, which efficiently facilitates resting sweat collection without external stimulation when integrated into the microfluidic channels of a sweat-sensing patch. The microfluidic sweat-sensing patch, fabricated with laser-cut technology, features a sandwich structure that enables the measurement of sweat rate and chloride ion concentration while minimizing interference from electrochemical reactions. Additionally, a colorimetric module utilizing glucose oxidase and peroxidase is also integrated into the platform for cost-effective and efficient glucose detection through a color change that can be quantified via RGB analysis. The hydrogel interface, characterized by its optimal thickness and water content, exhibits superior absorption capability for efficient sweat collection and retention, with a negligible effect on the dilution of sweat components. This hydrogel-interfaced microfluidic platform demonstrates high efficiency in sweat collection and multi-biomarker analysis, offering a non-invasive, real-time solution for health monitoring. Its low-cost and wearable design highlights its potential for broad applications in personalized healthcare.
ISSN:2079-6374