Extraction, structural characterization, and in vitro immunomodulatory activities of Saccharomyces cerevisiae spore wall β-glucan
Saccharomyces cerevisiae spores exposing β-glucan layers exhibit notable immunomodulatory properties, suggesting their potential as a natural and efficient source of β-glucans. However, studies on the application of spore wall β-glucans are limited, and their precise structural characteristics and i...
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Elsevier
2025-08-01
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| Series: | Journal of Agriculture and Food Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666154325004879 |
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| author | Mengqing Yan Xue Wang Jingtao Zhang Shuheng Yang Cong Pan Shiwei Liu Jia Liu Youqiang Yu Fangli Ding Shenglin Duan Huoqing Huang Guoyu Liu Peng Yuan |
| author_facet | Mengqing Yan Xue Wang Jingtao Zhang Shuheng Yang Cong Pan Shiwei Liu Jia Liu Youqiang Yu Fangli Ding Shenglin Duan Huoqing Huang Guoyu Liu Peng Yuan |
| author_sort | Mengqing Yan |
| collection | DOAJ |
| description | Saccharomyces cerevisiae spores exposing β-glucan layers exhibit notable immunomodulatory properties, suggesting their potential as a natural and efficient source of β-glucans. However, studies on the application of spore wall β-glucans are limited, and their precise structural characteristics and immunological mechanisms remain poorly understood. This study aimed to investigate the structural characteristics and immunomodulatory properties of β-glucan extracted from the yeast spores (YSG) and compare them with those of β-glucan derived from vegetative cells (YG). Similar to YG, YSG consists of a backbone chain of β-(1,3) glucan and side chains of β-(1,6) glucan with a natural triplex structure. However, there are differences in the branching ratio and helical structure between YSG and YG. YSG has fewer branches and a less pronounced triple helix structure than YG. The particle size (222.4 nm) and molecular weight (Mw: 1392.27 kDa) of YSG were found to be significantly smaller than those of YG. Additionally, YSG can bind to the Dectin-1 receptor protein on the surface of macrophages, activating the Syk/CARD9/NF-κB pathway to promote the expression of iNOS and cytokine mRNAs, thereby facilitating the release of NO and related cytokines for increased immunostimulatory effects. Collectively, our results provide foundational insights into the potential of yeast spore wall β-glucan as a functional immunostimulatory agent. |
| format | Article |
| id | doaj-art-3f42afb3f12b4be2b1de29e9ea0d73a3 |
| institution | DOAJ |
| issn | 2666-1543 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Agriculture and Food Research |
| spelling | doaj-art-3f42afb3f12b4be2b1de29e9ea0d73a32025-08-20T02:47:24ZengElsevierJournal of Agriculture and Food Research2666-15432025-08-012210211610.1016/j.jafr.2025.102116Extraction, structural characterization, and in vitro immunomodulatory activities of Saccharomyces cerevisiae spore wall β-glucanMengqing Yan0Xue Wang1Jingtao Zhang2Shuheng Yang3Cong Pan4Shiwei Liu5Jia Liu6Youqiang Yu7Fangli Ding8Shenglin Duan9Huoqing Huang10Guoyu Liu11Peng Yuan12China National Research Institute of Food and Fermentation Industries, Beijing, 100015, ChinaHeilongjiang Feihe Dairy Co., Ltd., Beijing, 100015, ChinaChina National Research Institute of Food and Fermentation Industries, Beijing, 100015, ChinaChina National Research Institute of Food and Fermentation Industries, Beijing, 100015, ChinaChina National Research Institute of Food and Fermentation Industries, Beijing, 100015, ChinaChina National Research Institute of Food and Fermentation Industries, Beijing, 100015, ChinaChina National Research Institute of Food and Fermentation Industries, Beijing, 100015, ChinaChina National Research Institute of Food and Fermentation Industries, Beijing, 100015, ChinaChina National Research Institute of Food and Fermentation Industries, Beijing, 100015, ChinaChina National Research Institute of Food and Fermentation Industries, Beijing, 100015, ChinaState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, ChinaChina National Research Institute of Food and Fermentation Industries, Beijing, 100015, China; Corresponding author.China National Research Institute of Food and Fermentation Industries, Beijing, 100015, China; Corresponding author.Saccharomyces cerevisiae spores exposing β-glucan layers exhibit notable immunomodulatory properties, suggesting their potential as a natural and efficient source of β-glucans. However, studies on the application of spore wall β-glucans are limited, and their precise structural characteristics and immunological mechanisms remain poorly understood. This study aimed to investigate the structural characteristics and immunomodulatory properties of β-glucan extracted from the yeast spores (YSG) and compare them with those of β-glucan derived from vegetative cells (YG). Similar to YG, YSG consists of a backbone chain of β-(1,3) glucan and side chains of β-(1,6) glucan with a natural triplex structure. However, there are differences in the branching ratio and helical structure between YSG and YG. YSG has fewer branches and a less pronounced triple helix structure than YG. The particle size (222.4 nm) and molecular weight (Mw: 1392.27 kDa) of YSG were found to be significantly smaller than those of YG. Additionally, YSG can bind to the Dectin-1 receptor protein on the surface of macrophages, activating the Syk/CARD9/NF-κB pathway to promote the expression of iNOS and cytokine mRNAs, thereby facilitating the release of NO and related cytokines for increased immunostimulatory effects. Collectively, our results provide foundational insights into the potential of yeast spore wall β-glucan as a functional immunostimulatory agent.http://www.sciencedirect.com/science/article/pii/S2666154325004879Saccharomyces cerevisiae sporeβ-GlucanStructural analysisImmune mechanism |
| spellingShingle | Mengqing Yan Xue Wang Jingtao Zhang Shuheng Yang Cong Pan Shiwei Liu Jia Liu Youqiang Yu Fangli Ding Shenglin Duan Huoqing Huang Guoyu Liu Peng Yuan Extraction, structural characterization, and in vitro immunomodulatory activities of Saccharomyces cerevisiae spore wall β-glucan Journal of Agriculture and Food Research Saccharomyces cerevisiae spore β-Glucan Structural analysis Immune mechanism |
| title | Extraction, structural characterization, and in vitro immunomodulatory activities of Saccharomyces cerevisiae spore wall β-glucan |
| title_full | Extraction, structural characterization, and in vitro immunomodulatory activities of Saccharomyces cerevisiae spore wall β-glucan |
| title_fullStr | Extraction, structural characterization, and in vitro immunomodulatory activities of Saccharomyces cerevisiae spore wall β-glucan |
| title_full_unstemmed | Extraction, structural characterization, and in vitro immunomodulatory activities of Saccharomyces cerevisiae spore wall β-glucan |
| title_short | Extraction, structural characterization, and in vitro immunomodulatory activities of Saccharomyces cerevisiae spore wall β-glucan |
| title_sort | extraction structural characterization and in vitro immunomodulatory activities of saccharomyces cerevisiae spore wall β glucan |
| topic | Saccharomyces cerevisiae spore β-Glucan Structural analysis Immune mechanism |
| url | http://www.sciencedirect.com/science/article/pii/S2666154325004879 |
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