Mesenchymal Stromal Cells Cultured in Serum from Heart Failure Patients Are More Resistant to Simulated Chronic and Acute Stress
Despite regulatory issues surrounding the use of animal-derived cell culture supplements, most clinical cardiac cell therapy trials using mesenchymal stromal cells (MSCs) still rely on fetal bovine serum (FBS) for cell expansion before transplantation. We sought to investigate the effect of human se...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/5832460 |
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| _version_ | 1832562315710431232 |
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| author | Timo Z. Nazari-Shafti Zhiyi Xu Andreas Matthäus Bader Georg Henke Kristin Klose Volkmar Falk Christof Stamm |
| author_facet | Timo Z. Nazari-Shafti Zhiyi Xu Andreas Matthäus Bader Georg Henke Kristin Klose Volkmar Falk Christof Stamm |
| author_sort | Timo Z. Nazari-Shafti |
| collection | DOAJ |
| description | Despite regulatory issues surrounding the use of animal-derived cell culture supplements, most clinical cardiac cell therapy trials using mesenchymal stromal cells (MSCs) still rely on fetal bovine serum (FBS) for cell expansion before transplantation. We sought to investigate the effect of human serum from heart failure patients (HFS) on cord blood MSCs (CB-MSCs) during short-term culture under regular conditions and during simulated acute and chronic stress. Cell survival, proliferation, metabolic activity, and apoptosis were quantified, and gene expression profiles of selected apoptosis and cell cycle regulators were determined. Compared to FBS, HFS and serum from healthy donors (CS) showed similar effects by substantially increasing cell survival during chronic and acute stress and by increasing cell yields 5 days after acute stress. Shortly after the termination of acute stress, both HFS and CS resulted in a marked decrease in apoptotic cells. Transcriptome analysis suggested a decrease in TNF-mediated induction of caspases and decreased activation of mitochondrial apoptosis. Our data confirm that human serum from both healthy donors and heart failure patients results in increased cell yields and increased resistance to cellular stress signals. Therefore, we consider autologous serum a valid alternative to FBS in cell-based therapies addressing severe heart disease. |
| format | Article |
| id | doaj-art-3f388477e5674288af7393f61aabcb4c |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-3f388477e5674288af7393f61aabcb4c2025-02-03T01:22:55ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/58324605832460Mesenchymal Stromal Cells Cultured in Serum from Heart Failure Patients Are More Resistant to Simulated Chronic and Acute StressTimo Z. Nazari-Shafti0Zhiyi Xu1Andreas Matthäus Bader2Georg Henke3Kristin Klose4Volkmar Falk5Christof Stamm6Deutsches Herzzentrum Berlin (DHZB), Berlin, GermanyBerlin Center for Regenerative Therapies (BCRT), Berlin, GermanyDeutsches Herzzentrum Berlin (DHZB), Berlin, GermanyBerlin Center for Regenerative Therapies (BCRT), Berlin, GermanyBerlin Center for Regenerative Therapies (BCRT), Berlin, GermanyDeutsches Herzzentrum Berlin (DHZB), Berlin, GermanyDeutsches Herzzentrum Berlin (DHZB), Berlin, GermanyDespite regulatory issues surrounding the use of animal-derived cell culture supplements, most clinical cardiac cell therapy trials using mesenchymal stromal cells (MSCs) still rely on fetal bovine serum (FBS) for cell expansion before transplantation. We sought to investigate the effect of human serum from heart failure patients (HFS) on cord blood MSCs (CB-MSCs) during short-term culture under regular conditions and during simulated acute and chronic stress. Cell survival, proliferation, metabolic activity, and apoptosis were quantified, and gene expression profiles of selected apoptosis and cell cycle regulators were determined. Compared to FBS, HFS and serum from healthy donors (CS) showed similar effects by substantially increasing cell survival during chronic and acute stress and by increasing cell yields 5 days after acute stress. Shortly after the termination of acute stress, both HFS and CS resulted in a marked decrease in apoptotic cells. Transcriptome analysis suggested a decrease in TNF-mediated induction of caspases and decreased activation of mitochondrial apoptosis. Our data confirm that human serum from both healthy donors and heart failure patients results in increased cell yields and increased resistance to cellular stress signals. Therefore, we consider autologous serum a valid alternative to FBS in cell-based therapies addressing severe heart disease.http://dx.doi.org/10.1155/2018/5832460 |
| spellingShingle | Timo Z. Nazari-Shafti Zhiyi Xu Andreas Matthäus Bader Georg Henke Kristin Klose Volkmar Falk Christof Stamm Mesenchymal Stromal Cells Cultured in Serum from Heart Failure Patients Are More Resistant to Simulated Chronic and Acute Stress Stem Cells International |
| title | Mesenchymal Stromal Cells Cultured in Serum from Heart Failure Patients Are More Resistant to Simulated Chronic and Acute Stress |
| title_full | Mesenchymal Stromal Cells Cultured in Serum from Heart Failure Patients Are More Resistant to Simulated Chronic and Acute Stress |
| title_fullStr | Mesenchymal Stromal Cells Cultured in Serum from Heart Failure Patients Are More Resistant to Simulated Chronic and Acute Stress |
| title_full_unstemmed | Mesenchymal Stromal Cells Cultured in Serum from Heart Failure Patients Are More Resistant to Simulated Chronic and Acute Stress |
| title_short | Mesenchymal Stromal Cells Cultured in Serum from Heart Failure Patients Are More Resistant to Simulated Chronic and Acute Stress |
| title_sort | mesenchymal stromal cells cultured in serum from heart failure patients are more resistant to simulated chronic and acute stress |
| url | http://dx.doi.org/10.1155/2018/5832460 |
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