CRISPR/Cas9-Mediated Editing of a <i>NODULATION SIGNALING PATHWAY 1</i> Homolog Alters the Production of Strigolactones in Sunflower Roots
Sunflower (<i>Helianthus annuus</i> L.) is specifically infected by an obligatory root parasitic plant <i>Orobanche cumana Wallr.</i> (sunflower broomrape), which causes significant losses of sunflower yield. Breeding of sunflower varieties resistant to broomrape is an import...
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MDPI AG
2025-01-01
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| author | Maria A. Lebedeva Maria S. Gancheva Maksim R. Losev Sofia V. Sokornova Oleg S. Yuzikhin Anna A. Krutikova Kirill V. Plemyashov Lyudmila A. Lutova |
| author_facet | Maria A. Lebedeva Maria S. Gancheva Maksim R. Losev Sofia V. Sokornova Oleg S. Yuzikhin Anna A. Krutikova Kirill V. Plemyashov Lyudmila A. Lutova |
| author_sort | Maria A. Lebedeva |
| collection | DOAJ |
| description | Sunflower (<i>Helianthus annuus</i> L.) is specifically infected by an obligatory root parasitic plant <i>Orobanche cumana Wallr.</i> (sunflower broomrape), which causes significant losses of sunflower yield. Breeding of sunflower varieties resistant to broomrape is an important challenge for agriculture. However, the selection of new resistant sunflower varieties was accompanied by the emergence of new virulent races of broomrape, which overcame the effect of resistance genes. Unraveling the molecular mechanisms underlying the resistance to broomrape in sunflowers should facilitate the development of new sunflower varieties with complex resistance to broomrape using genome editing technology. Here, we used CRISPR/Cas9-mediated genome editing in sunflower hairy roots for a specific knock-out of the gene encoding a GRAS transcription factor (HaNSP1a), acting as a possible regulator of strigolactone biosynthesis, a class of phytohormones known to induce the germination of broomrape seeds. According to HPLC-IT-TOF/MS analysis, the levels of orobanchol were decreased in the genetically modified roots with knock-out of the <i>HaNSP1a</i> gene, whereas, in contrast, 5-deoxystrigol levels were increased in the roots with <i>HaNSP1a</i> knock-out, suggesting the role of <i>HaNSP1a</i> in the regulation of the strigolactone biosynthetic pathway. The experimental approach described here could be used in further studies to test the effect of gene knock-out on the development of resistance to <i>O. cumana</i> in sunflowers. |
| format | Article |
| id | doaj-art-3f377c6408b04406a65c37ec56455305 |
| institution | Kabale University |
| issn | 2073-4395 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj-art-3f377c6408b04406a65c37ec564553052025-01-24T13:16:51ZengMDPI AGAgronomy2073-43952025-01-0115112910.3390/agronomy15010129CRISPR/Cas9-Mediated Editing of a <i>NODULATION SIGNALING PATHWAY 1</i> Homolog Alters the Production of Strigolactones in Sunflower RootsMaria A. Lebedeva0Maria S. Gancheva1Maksim R. Losev2Sofia V. Sokornova3Oleg S. Yuzikhin4Anna A. Krutikova5Kirill V. Plemyashov6Lyudmila A. Lutova7Department of Genetics and Biotechnology, Saint Petersburg State University, Universitetskaya Emb. 7/9, St. Petersburg 199034, RussiaDepartment of Genetics and Biotechnology, Saint Petersburg State University, Universitetskaya Emb. 7/9, St. Petersburg 199034, RussiaDepartment of Genetics and Biotechnology, Saint Petersburg State University, Universitetskaya Emb. 7/9, St. Petersburg 199034, RussiaFederal State Budget Scientific Institution “All-Russian Institute of Plant Protection”, (FSBSI VIZR), Podbelskogo, 3, St. Petersburg-Pushkin 196608, RussiaFederal State Budget Scientific Institution “All-Russian Institute of Plant Protection”, (FSBSI VIZR), Podbelskogo, 3, St. Petersburg-Pushkin 196608, RussiaDepartment of Genetic and Reproductive Biotechnology, St. Petersburg State University of Veterinary Medicine, Chernigovskaya St., 5, St. Petersburg 196084, RussiaDepartment of Genetic and Reproductive Biotechnology, St. Petersburg State University of Veterinary Medicine, Chernigovskaya St., 5, St. Petersburg 196084, RussiaDepartment of Genetics and Biotechnology, Saint Petersburg State University, Universitetskaya Emb. 7/9, St. Petersburg 199034, RussiaSunflower (<i>Helianthus annuus</i> L.) is specifically infected by an obligatory root parasitic plant <i>Orobanche cumana Wallr.</i> (sunflower broomrape), which causes significant losses of sunflower yield. Breeding of sunflower varieties resistant to broomrape is an important challenge for agriculture. However, the selection of new resistant sunflower varieties was accompanied by the emergence of new virulent races of broomrape, which overcame the effect of resistance genes. Unraveling the molecular mechanisms underlying the resistance to broomrape in sunflowers should facilitate the development of new sunflower varieties with complex resistance to broomrape using genome editing technology. Here, we used CRISPR/Cas9-mediated genome editing in sunflower hairy roots for a specific knock-out of the gene encoding a GRAS transcription factor (HaNSP1a), acting as a possible regulator of strigolactone biosynthesis, a class of phytohormones known to induce the germination of broomrape seeds. According to HPLC-IT-TOF/MS analysis, the levels of orobanchol were decreased in the genetically modified roots with knock-out of the <i>HaNSP1a</i> gene, whereas, in contrast, 5-deoxystrigol levels were increased in the roots with <i>HaNSP1a</i> knock-out, suggesting the role of <i>HaNSP1a</i> in the regulation of the strigolactone biosynthetic pathway. The experimental approach described here could be used in further studies to test the effect of gene knock-out on the development of resistance to <i>O. cumana</i> in sunflowers.https://www.mdpi.com/2073-4395/15/1/129sunflowergenome editingbroomrapestrigolactonescommon symbiotic pathway |
| spellingShingle | Maria A. Lebedeva Maria S. Gancheva Maksim R. Losev Sofia V. Sokornova Oleg S. Yuzikhin Anna A. Krutikova Kirill V. Plemyashov Lyudmila A. Lutova CRISPR/Cas9-Mediated Editing of a <i>NODULATION SIGNALING PATHWAY 1</i> Homolog Alters the Production of Strigolactones in Sunflower Roots Agronomy sunflower genome editing broomrape strigolactones common symbiotic pathway |
| title | CRISPR/Cas9-Mediated Editing of a <i>NODULATION SIGNALING PATHWAY 1</i> Homolog Alters the Production of Strigolactones in Sunflower Roots |
| title_full | CRISPR/Cas9-Mediated Editing of a <i>NODULATION SIGNALING PATHWAY 1</i> Homolog Alters the Production of Strigolactones in Sunflower Roots |
| title_fullStr | CRISPR/Cas9-Mediated Editing of a <i>NODULATION SIGNALING PATHWAY 1</i> Homolog Alters the Production of Strigolactones in Sunflower Roots |
| title_full_unstemmed | CRISPR/Cas9-Mediated Editing of a <i>NODULATION SIGNALING PATHWAY 1</i> Homolog Alters the Production of Strigolactones in Sunflower Roots |
| title_short | CRISPR/Cas9-Mediated Editing of a <i>NODULATION SIGNALING PATHWAY 1</i> Homolog Alters the Production of Strigolactones in Sunflower Roots |
| title_sort | crispr cas9 mediated editing of a i nodulation signaling pathway 1 i homolog alters the production of strigolactones in sunflower roots |
| topic | sunflower genome editing broomrape strigolactones common symbiotic pathway |
| url | https://www.mdpi.com/2073-4395/15/1/129 |
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