The effects of Lepidium meyenii on MSG-induced subfertility in male Wistar rats: Targeting oxidative stress, pro-inflammatory cytokines, steroidogenic enzymes, and reproductive hormones regulation
Objective and rationale: Although monosodium glutamate (MSG), commonly used to enrich food flavour, is generally considered safe for consumption, evidence has shown its adverse effects on physiological functions, including male reproduction, when consumed excessively. Lepidium meyenii (Maca), on the...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-02-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844025006267 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832582085885296640 |
|---|---|
| author | Temidayo S. Omolaoye Surendra Singh Rawat Naglaa Gamil Shehab Eslam El Nebrisi Stefan S. Du Plessis |
| author_facet | Temidayo S. Omolaoye Surendra Singh Rawat Naglaa Gamil Shehab Eslam El Nebrisi Stefan S. Du Plessis |
| author_sort | Temidayo S. Omolaoye |
| collection | DOAJ |
| description | Objective and rationale: Although monosodium glutamate (MSG), commonly used to enrich food flavour, is generally considered safe for consumption, evidence has shown its adverse effects on physiological functions, including male reproduction, when consumed excessively. Lepidium meyenii (Maca), on the other hand, a plant belonging to the Brassicaceae family, has been shown to have diverse health benefits. The current study investigated pathways through which MSG could induce subfertility, and whether treatment with Maca root extract would proffer amelioration via those routes. Methods: Eighteen male Wistar rats were randomly divided into three groups. Animals were orally (oral gavage) administered MSG (900 mg/kg/day for 45days), MSG and Maca (900 mg/kg/day of MSG and 500 mg/kg/day of black Lepidium meyenii root extract), or water only (control group), for a total period of 80 days. MSG was administered for the first 45 days, followed by Maca or water for the subsequent 35 days. Spermatozoa were collected from the caudal epididymis for sperm assessments. The testis and prostate were used for biochemical assays and immunofluorescent localization of biomarkers of interest. Results: MSG administration significantly reduced total and progressive sperm motility (p < 0.05), decreased total antioxidant capacity (TAC) (p < 0.001), increased mRNA expression of TNF-α, IL-6, IL-1β in the testis and prostate (p < 0.05), protein expression of GP130 in the testis, reduced serum testosterone, and altered expression of steroidogenic markers. These adverse effects were mildly ameliorated upon treatment with Maca. Conclusion: Findings highlights how MSG could cause male reproductive dysfunction via reduction of TAC, increase in pro-inflammatory cytokines, alter reproductive hormones and reduced protein expression of steroidogenic markers. Treatment with Maca significantly improved sperm motility, possibly by enhancing TAC levels and reducing proinflammatory cytokines. However, this hypothesis needs further investigation. |
| format | Article |
| id | doaj-art-3f35d6bbd8cd4fc28e7699ec5892e385 |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-3f35d6bbd8cd4fc28e7699ec5892e3852025-01-30T05:14:40ZengElsevierHeliyon2405-84402025-02-01113e42246The effects of Lepidium meyenii on MSG-induced subfertility in male Wistar rats: Targeting oxidative stress, pro-inflammatory cytokines, steroidogenic enzymes, and reproductive hormones regulationTemidayo S. Omolaoye0Surendra Singh Rawat1Naglaa Gamil Shehab2Eslam El Nebrisi3Stefan S. Du Plessis4College of Medicine, Mohammed Bin Rashid University of Medicine and Health Sciences, Dubai Health, DHCC, P.O.Box 505055, Dubai, United Arab Emirates; Corresponding author.College of Medicine, Mohammed Bin Rashid University of Medicine and Health Sciences, Dubai Health, DHCC, P.O.Box 505055, Dubai, United Arab Emirates; Research and Graduate Studies, Mohammed Bin Rashid University of Medicine and Health Sciences, Dubai Health, Dubai, United Arab Emirates; Pharmaceutical Sciences Department, College of Pharmacy, Dubai Medical University, Dubai, 19099, United Arab Emirates; Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt; Department of Biomedical Sciences, Dubai Medical College for Girls, Dubai Medical University, Dubai, 20170, United Arab EmiratesPharmaceutical Sciences Department, College of Pharmacy, Dubai Medical University, Dubai, 19099, United Arab EmiratesDepartment of Biomedical Sciences, Dubai Medical College for Girls, Dubai Medical University, Dubai, 20170, United Arab EmiratesCollege of Medicine, Mohammed Bin Rashid University of Medicine and Health Sciences, Dubai Health, DHCC, P.O.Box 505055, Dubai, United Arab EmiratesObjective and rationale: Although monosodium glutamate (MSG), commonly used to enrich food flavour, is generally considered safe for consumption, evidence has shown its adverse effects on physiological functions, including male reproduction, when consumed excessively. Lepidium meyenii (Maca), on the other hand, a plant belonging to the Brassicaceae family, has been shown to have diverse health benefits. The current study investigated pathways through which MSG could induce subfertility, and whether treatment with Maca root extract would proffer amelioration via those routes. Methods: Eighteen male Wistar rats were randomly divided into three groups. Animals were orally (oral gavage) administered MSG (900 mg/kg/day for 45days), MSG and Maca (900 mg/kg/day of MSG and 500 mg/kg/day of black Lepidium meyenii root extract), or water only (control group), for a total period of 80 days. MSG was administered for the first 45 days, followed by Maca or water for the subsequent 35 days. Spermatozoa were collected from the caudal epididymis for sperm assessments. The testis and prostate were used for biochemical assays and immunofluorescent localization of biomarkers of interest. Results: MSG administration significantly reduced total and progressive sperm motility (p < 0.05), decreased total antioxidant capacity (TAC) (p < 0.001), increased mRNA expression of TNF-α, IL-6, IL-1β in the testis and prostate (p < 0.05), protein expression of GP130 in the testis, reduced serum testosterone, and altered expression of steroidogenic markers. These adverse effects were mildly ameliorated upon treatment with Maca. Conclusion: Findings highlights how MSG could cause male reproductive dysfunction via reduction of TAC, increase in pro-inflammatory cytokines, alter reproductive hormones and reduced protein expression of steroidogenic markers. Treatment with Maca significantly improved sperm motility, possibly by enhancing TAC levels and reducing proinflammatory cytokines. However, this hypothesis needs further investigation.http://www.sciencedirect.com/science/article/pii/S2405844025006267Monosodium glutamateMacaLepidium meyeniiMale subfertilityOxidative stressSteroidogenesis |
| spellingShingle | Temidayo S. Omolaoye Surendra Singh Rawat Naglaa Gamil Shehab Eslam El Nebrisi Stefan S. Du Plessis The effects of Lepidium meyenii on MSG-induced subfertility in male Wistar rats: Targeting oxidative stress, pro-inflammatory cytokines, steroidogenic enzymes, and reproductive hormones regulation Heliyon Monosodium glutamate Maca Lepidium meyenii Male subfertility Oxidative stress Steroidogenesis |
| title | The effects of Lepidium meyenii on MSG-induced subfertility in male Wistar rats: Targeting oxidative stress, pro-inflammatory cytokines, steroidogenic enzymes, and reproductive hormones regulation |
| title_full | The effects of Lepidium meyenii on MSG-induced subfertility in male Wistar rats: Targeting oxidative stress, pro-inflammatory cytokines, steroidogenic enzymes, and reproductive hormones regulation |
| title_fullStr | The effects of Lepidium meyenii on MSG-induced subfertility in male Wistar rats: Targeting oxidative stress, pro-inflammatory cytokines, steroidogenic enzymes, and reproductive hormones regulation |
| title_full_unstemmed | The effects of Lepidium meyenii on MSG-induced subfertility in male Wistar rats: Targeting oxidative stress, pro-inflammatory cytokines, steroidogenic enzymes, and reproductive hormones regulation |
| title_short | The effects of Lepidium meyenii on MSG-induced subfertility in male Wistar rats: Targeting oxidative stress, pro-inflammatory cytokines, steroidogenic enzymes, and reproductive hormones regulation |
| title_sort | effects of lepidium meyenii on msg induced subfertility in male wistar rats targeting oxidative stress pro inflammatory cytokines steroidogenic enzymes and reproductive hormones regulation |
| topic | Monosodium glutamate Maca Lepidium meyenii Male subfertility Oxidative stress Steroidogenesis |
| url | http://www.sciencedirect.com/science/article/pii/S2405844025006267 |
| work_keys_str_mv | AT temidayosomolaoye theeffectsoflepidiummeyeniionmsginducedsubfertilityinmalewistarratstargetingoxidativestressproinflammatorycytokinessteroidogenicenzymesandreproductivehormonesregulation AT surendrasinghrawat theeffectsoflepidiummeyeniionmsginducedsubfertilityinmalewistarratstargetingoxidativestressproinflammatorycytokinessteroidogenicenzymesandreproductivehormonesregulation AT naglaagamilshehab theeffectsoflepidiummeyeniionmsginducedsubfertilityinmalewistarratstargetingoxidativestressproinflammatorycytokinessteroidogenicenzymesandreproductivehormonesregulation AT eslamelnebrisi theeffectsoflepidiummeyeniionmsginducedsubfertilityinmalewistarratstargetingoxidativestressproinflammatorycytokinessteroidogenicenzymesandreproductivehormonesregulation AT stefansduplessis theeffectsoflepidiummeyeniionmsginducedsubfertilityinmalewistarratstargetingoxidativestressproinflammatorycytokinessteroidogenicenzymesandreproductivehormonesregulation AT temidayosomolaoye effectsoflepidiummeyeniionmsginducedsubfertilityinmalewistarratstargetingoxidativestressproinflammatorycytokinessteroidogenicenzymesandreproductivehormonesregulation AT surendrasinghrawat effectsoflepidiummeyeniionmsginducedsubfertilityinmalewistarratstargetingoxidativestressproinflammatorycytokinessteroidogenicenzymesandreproductivehormonesregulation AT naglaagamilshehab effectsoflepidiummeyeniionmsginducedsubfertilityinmalewistarratstargetingoxidativestressproinflammatorycytokinessteroidogenicenzymesandreproductivehormonesregulation AT eslamelnebrisi effectsoflepidiummeyeniionmsginducedsubfertilityinmalewistarratstargetingoxidativestressproinflammatorycytokinessteroidogenicenzymesandreproductivehormonesregulation AT stefansduplessis effectsoflepidiummeyeniionmsginducedsubfertilityinmalewistarratstargetingoxidativestressproinflammatorycytokinessteroidogenicenzymesandreproductivehormonesregulation |