Effect of colistin-tigecycline combination on colistin-resistant and carbapenem-resistant Klebsiella pneumoniae and Acinetobacter baumannii
ABSTRACT There is a critical need for treatment strategies to combat carbapenem-resistant Gram-negative pathogens. This study investigates the efficacy of combining low concentrations of colistin with tigecycline against colistin- and carbapenem-resistant Klebsiella pneumoniae and Acinetobacter baum...
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American Society for Microbiology
2025-02-01
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| Series: | Microbiology Spectrum |
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| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.02021-24 |
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| author | Suyeon Park Yanhong Jin Kwan Soo Ko |
| author_facet | Suyeon Park Yanhong Jin Kwan Soo Ko |
| author_sort | Suyeon Park |
| collection | DOAJ |
| description | ABSTRACT There is a critical need for treatment strategies to combat carbapenem-resistant Gram-negative pathogens. This study investigates the efficacy of combining low concentrations of colistin with tigecycline against colistin- and carbapenem-resistant Klebsiella pneumoniae and Acinetobacter baumannii strains. We used two strains of KPC-2-producing K. pneumoniae and OXA-23-producing A. baumannii, both of which are highly colistin-resistant. In the in vitro time-killing assays, monotherapy with colistin (2 mg/L) and tigecycline (4 or 8 mg/L) was ineffective against all strains. However, the combination of colistin (2 mg/L) and tigecycline (4 or 8 mg/L) inhibited bacterial growth. Survival analysis of Galleria mellonella larvae also demonstrated the efficacy of low concentrations of colistin and tigecycline against colistin- and carbapenem-resistant strains. All larvae survived when treated with a combination of colistin and tigecycline, whereas monotherapy with either colistin or tigecycline did not increase larval survival rates. This study proposes using a combination of tigecycline and colistin at clinical concentrations to treat carbapenem-resistant K. pneumoniae and A. baumannii, regardless of colistin resistance.IMPORTANCEColistin resistance in carbapenem-resistant Gram-negative pathogens is a serious challenge in clinical settings. Our study showed that low concentration of colistin can be effective when combined with tigecycline. It presents the possibility of an available treatment option for antibiotic-resistant pathogens. |
| format | Article |
| id | doaj-art-3f205e253bc8412dbb80d5c105c93dd7 |
| institution | Kabale University |
| issn | 2165-0497 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj-art-3f205e253bc8412dbb80d5c105c93dd72025-02-04T14:03:41ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972025-02-0113210.1128/spectrum.02021-24Effect of colistin-tigecycline combination on colistin-resistant and carbapenem-resistant Klebsiella pneumoniae and Acinetobacter baumanniiSuyeon Park0Yanhong Jin1Kwan Soo Ko2Department of Microbiology, Sungkyunkwan University School of Medicine, Suwon, South KoreaDepartment of Microbiology, Sungkyunkwan University School of Medicine, Suwon, South KoreaDepartment of Microbiology, Sungkyunkwan University School of Medicine, Suwon, South KoreaABSTRACT There is a critical need for treatment strategies to combat carbapenem-resistant Gram-negative pathogens. This study investigates the efficacy of combining low concentrations of colistin with tigecycline against colistin- and carbapenem-resistant Klebsiella pneumoniae and Acinetobacter baumannii strains. We used two strains of KPC-2-producing K. pneumoniae and OXA-23-producing A. baumannii, both of which are highly colistin-resistant. In the in vitro time-killing assays, monotherapy with colistin (2 mg/L) and tigecycline (4 or 8 mg/L) was ineffective against all strains. However, the combination of colistin (2 mg/L) and tigecycline (4 or 8 mg/L) inhibited bacterial growth. Survival analysis of Galleria mellonella larvae also demonstrated the efficacy of low concentrations of colistin and tigecycline against colistin- and carbapenem-resistant strains. All larvae survived when treated with a combination of colistin and tigecycline, whereas monotherapy with either colistin or tigecycline did not increase larval survival rates. This study proposes using a combination of tigecycline and colistin at clinical concentrations to treat carbapenem-resistant K. pneumoniae and A. baumannii, regardless of colistin resistance.IMPORTANCEColistin resistance in carbapenem-resistant Gram-negative pathogens is a serious challenge in clinical settings. Our study showed that low concentration of colistin can be effective when combined with tigecycline. It presents the possibility of an available treatment option for antibiotic-resistant pathogens.https://journals.asm.org/doi/10.1128/spectrum.02021-24colistincarbapenem resistancetigecyclineKlebsiella pneumoniaeAcinetobacter baumannii |
| spellingShingle | Suyeon Park Yanhong Jin Kwan Soo Ko Effect of colistin-tigecycline combination on colistin-resistant and carbapenem-resistant Klebsiella pneumoniae and Acinetobacter baumannii Microbiology Spectrum colistin carbapenem resistance tigecycline Klebsiella pneumoniae Acinetobacter baumannii |
| title | Effect of colistin-tigecycline combination on colistin-resistant and carbapenem-resistant Klebsiella pneumoniae and Acinetobacter baumannii |
| title_full | Effect of colistin-tigecycline combination on colistin-resistant and carbapenem-resistant Klebsiella pneumoniae and Acinetobacter baumannii |
| title_fullStr | Effect of colistin-tigecycline combination on colistin-resistant and carbapenem-resistant Klebsiella pneumoniae and Acinetobacter baumannii |
| title_full_unstemmed | Effect of colistin-tigecycline combination on colistin-resistant and carbapenem-resistant Klebsiella pneumoniae and Acinetobacter baumannii |
| title_short | Effect of colistin-tigecycline combination on colistin-resistant and carbapenem-resistant Klebsiella pneumoniae and Acinetobacter baumannii |
| title_sort | effect of colistin tigecycline combination on colistin resistant and carbapenem resistant klebsiella pneumoniae and acinetobacter baumannii |
| topic | colistin carbapenem resistance tigecycline Klebsiella pneumoniae Acinetobacter baumannii |
| url | https://journals.asm.org/doi/10.1128/spectrum.02021-24 |
| work_keys_str_mv | AT suyeonpark effectofcolistintigecyclinecombinationoncolistinresistantandcarbapenemresistantklebsiellapneumoniaeandacinetobacterbaumannii AT yanhongjin effectofcolistintigecyclinecombinationoncolistinresistantandcarbapenemresistantklebsiellapneumoniaeandacinetobacterbaumannii AT kwansooko effectofcolistintigecyclinecombinationoncolistinresistantandcarbapenemresistantklebsiellapneumoniaeandacinetobacterbaumannii |