Expression profiles of the Tau-associated genes GSk3β, CAPN1, and CDK5R1 in the brain cortex of aged female cynomolgus monkeys with cognitive impairment
Background: Alzheimer's disease (AD) is characterized by the buildup and aggregation of misfolded proteins in the brain, including amyloid-β (Aβ) and hyperphosphorylated tau. The hyperphosphorylation state of Tau protein plays an important role in the development of AD. Our previous studie...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Tripoli University
2025-03-01
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| Series: | Open Veterinary Journal |
| Subjects: | |
| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=228287 |
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| Summary: | Background:
Alzheimer's disease (AD) is characterized by the buildup and aggregation of misfolded proteins in the brain, including amyloid-β (Aβ) and hyperphosphorylated tau. The hyperphosphorylation state of Tau protein plays an important role in the development of AD. Our previous studies developed and characterized the cynomolgus monkey as a spontaneous animal model of AD.
Aim:
We demonstrated the validity of the model through experimental investigations of the relationships between cognitive decline and the neuro-pathy of AD. There is, however, little information about the expression actvity of hyperphosphorylated tau related genes in various brain areas in the in cynomolgus monkey spontaneous AD model.
Methods:
In the present study, total RNA was extracted from archived cortex and hippocampus tissues from the brains of two groups of cynomolgus monkeys, adult (10-12 years old, n=5) and aged (> 20 years old, n=4). The expression of the tau-protein-associated genes GSK3β, CAPN1, and CDK5R1 was evaluated using RT-qPCR technique
Results:
The expression of all three genes was increased up to five fold in the brain cortical area of the aged subjects compared to the adults
Conclusion:
The results add weight to the utility of cynomolgus macaques as a valid spontaneous model in translational preclinical research involving studies of the effect of aging on the formation of hyperphosphorylated tau protein, which causes AD-related lesions in the brain. [Open Vet J 2025; 15(3.000): 1150-1156] |
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| ISSN: | 2226-4485 2218-6050 |