Molecular identification of chitinase and its function in growth of Odontobutis potamophila
Chitinases are pivotal enzymes for the degradation of chitin, which play a role in food digestion. However, the functional roles of chitinases in teleosts remain under-explored. In this study, a full-length cDNA of chitinase gene (namely, OpCht) was obtained from dark sleeper Odontobutis potamophila...
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Elsevier
2025-03-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513424006434 |
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| author | Siyu Lu Lin Du Ting Zhang Dan Wang Jinjuan Wan Guoxing Liu Yuning Hu Yu Xu Zhiqiang Xu Xuguang Li |
| author_facet | Siyu Lu Lin Du Ting Zhang Dan Wang Jinjuan Wan Guoxing Liu Yuning Hu Yu Xu Zhiqiang Xu Xuguang Li |
| author_sort | Siyu Lu |
| collection | DOAJ |
| description | Chitinases are pivotal enzymes for the degradation of chitin, which play a role in food digestion. However, the functional roles of chitinases in teleosts remain under-explored. In this study, a full-length cDNA of chitinase gene (namely, OpCht) was obtained from dark sleeper Odontobutis potamophila. The predicted amino acid sequence of OpCht consisted of a typical catalytic domain and a chitin-binding domain interconnected with a linker region. Phylogenetic analyses suggest that OpCht has appeared in the teleost-specific monophyletic branches (CHIO). Quantitative real-time PCR analyses showed that OpCht was mainly expressed in stomach, liver, intestine and increased significantly during the period of juvenile. The optimal gradient concentrations and intervals/efficiency of RNA interference (RNAi) in OpCht were established, the results demonstrated that RNAi significantly repressed the expression of OpCht and diminished the chitinase activity. After 8 weeks of long-term RNAi, knockdown of OpCht reduced the height of intestinal villus and the thickness of muscular layer, which led to a decline in weight gain rate (WGR), while the accompanying intestinal inflammation contributed to a decreased survival rate (SR) in O. potamophila. In conclusion, OpCht is implicated in chitin degradation and plays an important role in intestinal health and growth performance in O. potamophila. |
| format | Article |
| id | doaj-art-3eb99d1b67814288b7282373270174e2 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-3eb99d1b67814288b7282373270174e22025-02-06T05:12:07ZengElsevierAquaculture Reports2352-51342025-03-0140102555Molecular identification of chitinase and its function in growth of Odontobutis potamophilaSiyu Lu0Lin Du1Ting Zhang2Dan Wang3Jinjuan Wan4Guoxing Liu5Yuning Hu6Yu Xu7Zhiqiang Xu8Xuguang Li9Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Marine Science and Engineering, Nanjing Normal University, Nanjing 210023, China; Key Laboratory of Genetic Breeding and cultivation for Freshwater Crustacean, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing 210017, ChinaJiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Marine Science and Engineering, Nanjing Normal University, Nanjing 210023, China; Key Laboratory of Genetic Breeding and cultivation for Freshwater Crustacean, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing 210017, ChinaKey Laboratory of Genetic Breeding and cultivation for Freshwater Crustacean, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing 210017, ChinaKey Laboratory of Genetic Breeding and cultivation for Freshwater Crustacean, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing 210017, ChinaKey Laboratory of Genetic Breeding and cultivation for Freshwater Crustacean, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing 210017, ChinaKey Laboratory of Genetic Breeding and cultivation for Freshwater Crustacean, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing 210017, China; Low-temperature Germplasm Bank of Important Economic Fish (Freshwater Fisheries Research Institute of Jiangsu Province) of Jiangsu Provincial Science and Technology Resources (Agricultural Germplasm Resources) Coordination Service Platform, Nanjing, ChinaKey Laboratory of Genetic Breeding and cultivation for Freshwater Crustacean, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing 210017, ChinaKey Laboratory of Genetic Breeding and cultivation for Freshwater Crustacean, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing 210017, ChinaKey Laboratory of Genetic Breeding and cultivation for Freshwater Crustacean, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing 210017, ChinaKey Laboratory of Genetic Breeding and cultivation for Freshwater Crustacean, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing 210017, China; Correspondence to: Freshwater Fisheries Research Institute of Jiangsu Province, No. 90, South Lake East Street, Jianye District, Jiangsu Province 210017, China.Chitinases are pivotal enzymes for the degradation of chitin, which play a role in food digestion. However, the functional roles of chitinases in teleosts remain under-explored. In this study, a full-length cDNA of chitinase gene (namely, OpCht) was obtained from dark sleeper Odontobutis potamophila. The predicted amino acid sequence of OpCht consisted of a typical catalytic domain and a chitin-binding domain interconnected with a linker region. Phylogenetic analyses suggest that OpCht has appeared in the teleost-specific monophyletic branches (CHIO). Quantitative real-time PCR analyses showed that OpCht was mainly expressed in stomach, liver, intestine and increased significantly during the period of juvenile. The optimal gradient concentrations and intervals/efficiency of RNA interference (RNAi) in OpCht were established, the results demonstrated that RNAi significantly repressed the expression of OpCht and diminished the chitinase activity. After 8 weeks of long-term RNAi, knockdown of OpCht reduced the height of intestinal villus and the thickness of muscular layer, which led to a decline in weight gain rate (WGR), while the accompanying intestinal inflammation contributed to a decreased survival rate (SR) in O. potamophila. In conclusion, OpCht is implicated in chitin degradation and plays an important role in intestinal health and growth performance in O. potamophila.http://www.sciencedirect.com/science/article/pii/S2352513424006434Odontobutis potamophilaChitinaseRNA interferenceIntestinal healthGrowth performance |
| spellingShingle | Siyu Lu Lin Du Ting Zhang Dan Wang Jinjuan Wan Guoxing Liu Yuning Hu Yu Xu Zhiqiang Xu Xuguang Li Molecular identification of chitinase and its function in growth of Odontobutis potamophila Aquaculture Reports Odontobutis potamophila Chitinase RNA interference Intestinal health Growth performance |
| title | Molecular identification of chitinase and its function in growth of Odontobutis potamophila |
| title_full | Molecular identification of chitinase and its function in growth of Odontobutis potamophila |
| title_fullStr | Molecular identification of chitinase and its function in growth of Odontobutis potamophila |
| title_full_unstemmed | Molecular identification of chitinase and its function in growth of Odontobutis potamophila |
| title_short | Molecular identification of chitinase and its function in growth of Odontobutis potamophila |
| title_sort | molecular identification of chitinase and its function in growth of odontobutis potamophila |
| topic | Odontobutis potamophila Chitinase RNA interference Intestinal health Growth performance |
| url | http://www.sciencedirect.com/science/article/pii/S2352513424006434 |
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