Autoantibodies to Zinc Transporter 8 and SLC30A8 Genotype in Type 1 Diabetes Childhood: A Pioneering Study in North Africa
Background. Type 1 diabetes (T1D) occurs as a result of insulin deficiency due to destructive lesions of pancreatic β cells. In addition to classical autoantibodies (Abs) to islet cell antigens, antizinc transporter 8 Abs (ZnT8-Ab) have been recently described in T1D. Objective. As no data on ZnT8-A...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2022/2539871 |
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| author | Raouia Fakhfakh Sana Kmiha Safa Tahri Sawsan Feki Ferjeni Zouidi Olfa Abida Mongia Hachicha Thouraya Kammoun Hatem Masmoudi |
| author_facet | Raouia Fakhfakh Sana Kmiha Safa Tahri Sawsan Feki Ferjeni Zouidi Olfa Abida Mongia Hachicha Thouraya Kammoun Hatem Masmoudi |
| author_sort | Raouia Fakhfakh |
| collection | DOAJ |
| description | Background. Type 1 diabetes (T1D) occurs as a result of insulin deficiency due to destructive lesions of pancreatic β cells. In addition to classical autoantibodies (Abs) to islet cell antigens, antizinc transporter 8 Abs (ZnT8-Ab) have been recently described in T1D. Objective. As no data on ZnT8-Ab in Tunisian patients has been reported, we aim to evaluate the relationships between ZnT8-Ab, ZnT8 coding gene (SLC30A8) promoter polymorphism, and T1D risk in newly diagnosed children. Methods. ZnT8-Ab were measured in the serum of T1D newly affected children (n=156) who were admitted to the pediatric department of the Hedi Chaker University Hospital of Sfax. Rs13266634 was genotyped in T1D children and 79 of their first-degree parents. The SPSS software was used to analyze the serological data. Allelic association analysis was conducted with family-based association tests implemented in the FBAT program v1.5.1. Results. ZnT8-Ab was detected in 66/156 (42.3%) of T1D newly diagnosed children. Among them, 6 (9%) presented ZnT8-Ab as the only humoral marker. The inclusion of ZnT8-Ab increased the number of Ab-positive patients to 90% and reduced the negative ones by 27%. There was no evidence of any overtransmission of any allele of the rs13266634 C/T polymorphism from parents to affected T1D children, nor of any correlation with any clinical or serological parameter. After the T1D disease onset age adjustment, a significant association was observed with the C allele suggesting that it could have a susceptibility role. Conclusion. ZnT8-Ab appears as a relevant diagnostic marker for T1D in Tunisian children, especially at the onset of the disease as teenagers. |
| format | Article |
| id | doaj-art-3eb7b2cc73a34fbc97d9d2ddc3ae4eab |
| institution | Kabale University |
| issn | 2314-6753 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-3eb7b2cc73a34fbc97d9d2ddc3ae4eab2025-02-03T05:53:38ZengWileyJournal of Diabetes Research2314-67532022-01-01202210.1155/2022/2539871Autoantibodies to Zinc Transporter 8 and SLC30A8 Genotype in Type 1 Diabetes Childhood: A Pioneering Study in North AfricaRaouia Fakhfakh0Sana Kmiha1Safa Tahri2Sawsan Feki3Ferjeni Zouidi4Olfa Abida5Mongia Hachicha6Thouraya Kammoun7Hatem Masmoudi8AutoimmunityPediatrics DepartmentAutoimmunityAutoimmunityAutoimmunityAutoimmunityPediatrics DepartmentPediatrics DepartmentAutoimmunityBackground. Type 1 diabetes (T1D) occurs as a result of insulin deficiency due to destructive lesions of pancreatic β cells. In addition to classical autoantibodies (Abs) to islet cell antigens, antizinc transporter 8 Abs (ZnT8-Ab) have been recently described in T1D. Objective. As no data on ZnT8-Ab in Tunisian patients has been reported, we aim to evaluate the relationships between ZnT8-Ab, ZnT8 coding gene (SLC30A8) promoter polymorphism, and T1D risk in newly diagnosed children. Methods. ZnT8-Ab were measured in the serum of T1D newly affected children (n=156) who were admitted to the pediatric department of the Hedi Chaker University Hospital of Sfax. Rs13266634 was genotyped in T1D children and 79 of their first-degree parents. The SPSS software was used to analyze the serological data. Allelic association analysis was conducted with family-based association tests implemented in the FBAT program v1.5.1. Results. ZnT8-Ab was detected in 66/156 (42.3%) of T1D newly diagnosed children. Among them, 6 (9%) presented ZnT8-Ab as the only humoral marker. The inclusion of ZnT8-Ab increased the number of Ab-positive patients to 90% and reduced the negative ones by 27%. There was no evidence of any overtransmission of any allele of the rs13266634 C/T polymorphism from parents to affected T1D children, nor of any correlation with any clinical or serological parameter. After the T1D disease onset age adjustment, a significant association was observed with the C allele suggesting that it could have a susceptibility role. Conclusion. ZnT8-Ab appears as a relevant diagnostic marker for T1D in Tunisian children, especially at the onset of the disease as teenagers.http://dx.doi.org/10.1155/2022/2539871 |
| spellingShingle | Raouia Fakhfakh Sana Kmiha Safa Tahri Sawsan Feki Ferjeni Zouidi Olfa Abida Mongia Hachicha Thouraya Kammoun Hatem Masmoudi Autoantibodies to Zinc Transporter 8 and SLC30A8 Genotype in Type 1 Diabetes Childhood: A Pioneering Study in North Africa Journal of Diabetes Research |
| title | Autoantibodies to Zinc Transporter 8 and SLC30A8 Genotype in Type 1 Diabetes Childhood: A Pioneering Study in North Africa |
| title_full | Autoantibodies to Zinc Transporter 8 and SLC30A8 Genotype in Type 1 Diabetes Childhood: A Pioneering Study in North Africa |
| title_fullStr | Autoantibodies to Zinc Transporter 8 and SLC30A8 Genotype in Type 1 Diabetes Childhood: A Pioneering Study in North Africa |
| title_full_unstemmed | Autoantibodies to Zinc Transporter 8 and SLC30A8 Genotype in Type 1 Diabetes Childhood: A Pioneering Study in North Africa |
| title_short | Autoantibodies to Zinc Transporter 8 and SLC30A8 Genotype in Type 1 Diabetes Childhood: A Pioneering Study in North Africa |
| title_sort | autoantibodies to zinc transporter 8 and slc30a8 genotype in type 1 diabetes childhood a pioneering study in north africa |
| url | http://dx.doi.org/10.1155/2022/2539871 |
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