Detection and Characterization of β-Lactam Resistance in Bacillus cereus PTCC 1015
In the present study, detection, isolation, and characterization of β-lactamases from Bacillus cereus PTCC 1015 were investigated. B. cereus was inoculated in nutrient broth containing ampicillin (50 μg.ml−1) for 24 h (35°C, 200 rpm). Activity measurements were carried out against ampicillin (0.1 mg...
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| Format: | Article |
| Language: | English |
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Wiley
2004-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/tsw.2004.122 |
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| author | Javad Behravan Zeinab Rangsaaz |
| author_facet | Javad Behravan Zeinab Rangsaaz |
| author_sort | Javad Behravan |
| collection | DOAJ |
| description | In the present study, detection, isolation, and characterization of β-lactamases from Bacillus cereus PTCC 1015 were investigated. B. cereus was inoculated in nutrient broth containing ampicillin (50 μg.ml−1) for 24 h (35°C, 200 rpm). Activity measurements were carried out against ampicillin (0.1 mg.ml−1) and cephalexin (0.08 mg.ml−1) by a spectrophotometric method at different conditions (pH 6–10, temperatures 2545°C).Maximum penicillinase and cephalosporinase activity was observed at pH 7. The optimized temperatures for penicillinase and cephalosporinase activity were 30 and 40°C, respectively. At the above conditions, maximum enzymatic activity was calculated as 0.89 ± 0.014 and 0.037 ± 0.001 units against ampicillin and cephalexin. |
| format | Article |
| id | doaj-art-3e78f0e6ff49451fbe9771047535757a |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2004-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-3e78f0e6ff49451fbe9771047535757a2025-02-03T01:27:53ZengWileyThe Scientific World Journal1537-744X2004-01-01462262710.1100/tsw.2004.122Detection and Characterization of β-Lactam Resistance in Bacillus cereus PTCC 1015Javad Behravan0Zeinab Rangsaaz1Pharmaceutical Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, IRANBiotechnology Section, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IRANIn the present study, detection, isolation, and characterization of β-lactamases from Bacillus cereus PTCC 1015 were investigated. B. cereus was inoculated in nutrient broth containing ampicillin (50 μg.ml−1) for 24 h (35°C, 200 rpm). Activity measurements were carried out against ampicillin (0.1 mg.ml−1) and cephalexin (0.08 mg.ml−1) by a spectrophotometric method at different conditions (pH 6–10, temperatures 2545°C).Maximum penicillinase and cephalosporinase activity was observed at pH 7. The optimized temperatures for penicillinase and cephalosporinase activity were 30 and 40°C, respectively. At the above conditions, maximum enzymatic activity was calculated as 0.89 ± 0.014 and 0.037 ± 0.001 units against ampicillin and cephalexin.http://dx.doi.org/10.1100/tsw.2004.122 |
| spellingShingle | Javad Behravan Zeinab Rangsaaz Detection and Characterization of β-Lactam Resistance in Bacillus cereus PTCC 1015 The Scientific World Journal |
| title | Detection and Characterization of β-Lactam Resistance in Bacillus cereus PTCC 1015 |
| title_full | Detection and Characterization of β-Lactam Resistance in Bacillus cereus PTCC 1015 |
| title_fullStr | Detection and Characterization of β-Lactam Resistance in Bacillus cereus PTCC 1015 |
| title_full_unstemmed | Detection and Characterization of β-Lactam Resistance in Bacillus cereus PTCC 1015 |
| title_short | Detection and Characterization of β-Lactam Resistance in Bacillus cereus PTCC 1015 |
| title_sort | detection and characterization of β lactam resistance in bacillus cereus ptcc 1015 |
| url | http://dx.doi.org/10.1100/tsw.2004.122 |
| work_keys_str_mv | AT javadbehravan detectionandcharacterizationofblactamresistanceinbacilluscereusptcc1015 AT zeinabrangsaaz detectionandcharacterizationofblactamresistanceinbacilluscereusptcc1015 |