Advance technologies for DNA-protein interactions and future research prospect
DNA-protein interactions (DPIs) are essential for genome functioning, with billions of years of evolution shaping specific patterns of protein-DNA interactions to regulate gene networks in response to various stimuli. Over the years, scientists have developed numerous techniques to study these inter...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Crop Design |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772899424000314 |
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| author | Chengyi Qu Hao Du |
| author_facet | Chengyi Qu Hao Du |
| author_sort | Chengyi Qu |
| collection | DOAJ |
| description | DNA-protein interactions (DPIs) are essential for genome functioning, with billions of years of evolution shaping specific patterns of protein-DNA interactions to regulate gene networks in response to various stimuli. Over the years, scientists have developed numerous techniques to study these interactions. This review provides a historical overview of these methods, highlighting their advantages and disadvantages and offering the examples of recent applications. Our aim is to help researchers select the most appropriate technique on the basis of their working goals and capabilities. For the experimental design of DPIs assays, several kinds of techniques are relatively quicker or/and simpler, the precision and accuracy of these methods must be carefully considered to verify the DNA-protein interaction. The review also discusses the recent advances in the computational approaches for predicting DNA-protein bindings and diverse reporting systems. |
| format | Article |
| id | doaj-art-3e2a1fa6b6d443a499cf30f2f5cb2983 |
| institution | Kabale University |
| issn | 2772-8994 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Crop Design |
| spelling | doaj-art-3e2a1fa6b6d443a499cf30f2f5cb29832025-01-24T04:46:04ZengElsevierCrop Design2772-89942025-02-0141100082Advance technologies for DNA-protein interactions and future research prospectChengyi Qu0Hao Du1College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, ChinaCollege of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China; ZJU-Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou, 311215, China; Corresponding author. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China.DNA-protein interactions (DPIs) are essential for genome functioning, with billions of years of evolution shaping specific patterns of protein-DNA interactions to regulate gene networks in response to various stimuli. Over the years, scientists have developed numerous techniques to study these interactions. This review provides a historical overview of these methods, highlighting their advantages and disadvantages and offering the examples of recent applications. Our aim is to help researchers select the most appropriate technique on the basis of their working goals and capabilities. For the experimental design of DPIs assays, several kinds of techniques are relatively quicker or/and simpler, the precision and accuracy of these methods must be carefully considered to verify the DNA-protein interaction. The review also discusses the recent advances in the computational approaches for predicting DNA-protein bindings and diverse reporting systems.http://www.sciencedirect.com/science/article/pii/S2772899424000314DNA-protein interactionElectrophoretic mobility shift assayEnzyme-linked immunosorbent assayFootprintingCUT&RUNChromatin immunoprecipitation |
| spellingShingle | Chengyi Qu Hao Du Advance technologies for DNA-protein interactions and future research prospect Crop Design DNA-protein interaction Electrophoretic mobility shift assay Enzyme-linked immunosorbent assay Footprinting CUT&RUN Chromatin immunoprecipitation |
| title | Advance technologies for DNA-protein interactions and future research prospect |
| title_full | Advance technologies for DNA-protein interactions and future research prospect |
| title_fullStr | Advance technologies for DNA-protein interactions and future research prospect |
| title_full_unstemmed | Advance technologies for DNA-protein interactions and future research prospect |
| title_short | Advance technologies for DNA-protein interactions and future research prospect |
| title_sort | advance technologies for dna protein interactions and future research prospect |
| topic | DNA-protein interaction Electrophoretic mobility shift assay Enzyme-linked immunosorbent assay Footprinting CUT&RUN Chromatin immunoprecipitation |
| url | http://www.sciencedirect.com/science/article/pii/S2772899424000314 |
| work_keys_str_mv | AT chengyiqu advancetechnologiesfordnaproteininteractionsandfutureresearchprospect AT haodu advancetechnologiesfordnaproteininteractionsandfutureresearchprospect |