Application of Response Surface Methodology to Study the Combined Effect of Temperature, Time and pH on Antioxidant Activity of Cherry (Prunus avium) Honey

Response surface methodology (RSM) was employed to analyze the effect of independent variables viz. temperature, time and pH on antioxidant properties of cherry honey. Seventeen runs including five replicates were used to study the combined effect of temperature (60–80°C), time (10–15 min) and pH (3...

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Main Authors: Nayik Gulzar Ahmad, Nanda Vikas
Format: Article
Language:English
Published: Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences 2016-12-01
Series:Polish Journal of Food and Nutrition Sciences
Subjects:
Online Access:http://www.degruyter.com/view/j/pjfns.2016.66.issue-4/pjfns-2015-0055/pjfns-2015-0055.xml?format=INT
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author Nayik Gulzar Ahmad
Nanda Vikas
author_facet Nayik Gulzar Ahmad
Nanda Vikas
author_sort Nayik Gulzar Ahmad
collection DOAJ
description Response surface methodology (RSM) was employed to analyze the effect of independent variables viz. temperature, time and pH on antioxidant properties of cherry honey. Seventeen runs including five replicates were used to study the combined effect of temperature (60–80°C), time (10–15 min) and pH (3 to 6) on the antioxidant properties [radical scavenging activity (DPPH-RSA), total phenolic content (TPC) and total flavonoid content (TFC)] of cherry honey. Statistical analysis revealed that process variables significantly affected all the responses. All the three parameters viz. DPPH-RSA, TPC and TFC increased with increase in time and temperature. The antioxidant properties of cherry honey were significantly decreased with increase in pH from 3 to 6. The thermal treatment of honey at 80°C was found to be more effective than at 70 and 60°C. The results demonstrated that antioxidant activity significantly increased with formation of browning pigments.
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spelling doaj-art-3e252e1c72164f0580b577fc4f0d070d2025-02-02T13:38:08ZengInstitute of Animal Reproduction and Food Research of the Polish Academy of SciencesPolish Journal of Food and Nutrition Sciences2083-60072016-12-0166428729410.1515/pjfns-2015-0055pjfns-2015-0055Application of Response Surface Methodology to Study the Combined Effect of Temperature, Time and pH on Antioxidant Activity of Cherry (Prunus avium) HoneyNayik Gulzar Ahmad0Nanda Vikas1Department of Food Engineering and Technology, Sant Longowal Institute of Engineering and Technology, Longowal 148106 (Punjab) IndiaDepartment of Food Engineering and Technology, Sant Longowal Institute of Engineering and Technology, Longowal 148106 (Punjab) IndiaResponse surface methodology (RSM) was employed to analyze the effect of independent variables viz. temperature, time and pH on antioxidant properties of cherry honey. Seventeen runs including five replicates were used to study the combined effect of temperature (60–80°C), time (10–15 min) and pH (3 to 6) on the antioxidant properties [radical scavenging activity (DPPH-RSA), total phenolic content (TPC) and total flavonoid content (TFC)] of cherry honey. Statistical analysis revealed that process variables significantly affected all the responses. All the three parameters viz. DPPH-RSA, TPC and TFC increased with increase in time and temperature. The antioxidant properties of cherry honey were significantly decreased with increase in pH from 3 to 6. The thermal treatment of honey at 80°C was found to be more effective than at 70 and 60°C. The results demonstrated that antioxidant activity significantly increased with formation of browning pigments.http://www.degruyter.com/view/j/pjfns.2016.66.issue-4/pjfns-2015-0055/pjfns-2015-0055.xml?format=INTcherry honeyantioxidant activitytotal phenolic contenttotal flavonoid content
spellingShingle Nayik Gulzar Ahmad
Nanda Vikas
Application of Response Surface Methodology to Study the Combined Effect of Temperature, Time and pH on Antioxidant Activity of Cherry (Prunus avium) Honey
Polish Journal of Food and Nutrition Sciences
cherry honey
antioxidant activity
total phenolic content
total flavonoid content
title Application of Response Surface Methodology to Study the Combined Effect of Temperature, Time and pH on Antioxidant Activity of Cherry (Prunus avium) Honey
title_full Application of Response Surface Methodology to Study the Combined Effect of Temperature, Time and pH on Antioxidant Activity of Cherry (Prunus avium) Honey
title_fullStr Application of Response Surface Methodology to Study the Combined Effect of Temperature, Time and pH on Antioxidant Activity of Cherry (Prunus avium) Honey
title_full_unstemmed Application of Response Surface Methodology to Study the Combined Effect of Temperature, Time and pH on Antioxidant Activity of Cherry (Prunus avium) Honey
title_short Application of Response Surface Methodology to Study the Combined Effect of Temperature, Time and pH on Antioxidant Activity of Cherry (Prunus avium) Honey
title_sort application of response surface methodology to study the combined effect of temperature time and ph on antioxidant activity of cherry prunus avium honey
topic cherry honey
antioxidant activity
total phenolic content
total flavonoid content
url http://www.degruyter.com/view/j/pjfns.2016.66.issue-4/pjfns-2015-0055/pjfns-2015-0055.xml?format=INT
work_keys_str_mv AT nayikgulzarahmad applicationofresponsesurfacemethodologytostudythecombinedeffectoftemperaturetimeandphonantioxidantactivityofcherryprunusaviumhoney
AT nandavikas applicationofresponsesurfacemethodologytostudythecombinedeffectoftemperaturetimeandphonantioxidantactivityofcherryprunusaviumhoney