Surface-Enhanced Raman Spectroscopy (SERS) for characterization SARS-CoV-2
We used SERS with silver nanoparticles (AgNPs) as the active substrate to develop a, simple, quick, and accurate method for the detection and characterization SARS-CoV-2 without the need for RNA isolation and purification. Inactivated SARS-CoV-2 was used. The SERS signals were more than 105 times en...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer
2022-09-01
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| Series: | Journal of Saudi Chemical Society |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319610322001132 |
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| Summary: | We used SERS with silver nanoparticles (AgNPs) as the active substrate to develop a, simple, quick, and accurate method for the detection and characterization SARS-CoV-2 without the need for RNA isolation and purification. Inactivated SARS-CoV-2 was used. The SERS signals were more than 105 times enhanced than the normal Raman (NR) spectra. The SERS spectra of SARS-CoV-2 fingerprint revealed pronounced intensity signals of nucleic acids; aromatic amino acid side chains: 1007 cm−1 (Phe marker), 1095 cm−1 (CN and PO2− markers), 1580 cm−1 (Tyr, Trp markers). Vibrations of the protein main chain: 1144 cm−1 (CN and NH2 markers), 1221 cm−1 (CN and NH markers), 1270 cm−1 (NH2 marker), 1453 cm−1 (CHCH2 marker). All of these biomolecules could be adsorbed on the AgNPs surface's dense hot patches. The intensity of the SERS band varied with the concentration of SARS-CoV-2, with a virus detection limit of less than 103 vp/mL and RSDs of 20 %. |
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| ISSN: | 1319-6103 |