Oxidation state of bioavailable dissolved organic matter influences bacterioplankton respiration and growth efficiency
Abstract Oxygen consumption by oceanic microbes can predict respiration (CO2 production) but requires an assumed respiratory quotient (RQ; ΔO2/ΔCO2). Measured apparent RQs (ARQs) can be impacted by various processes, including nitrification and changes in dissolved organic matter (DOM) composition,...
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| Format: | Article |
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Nature Portfolio
2025-01-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-025-07574-2 |
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| author | Brandon M. Stephens Paolo Stincone Daniel Petras Chance J. English Keri Opalk Stephen Giovannoni Craig A. Carlson |
| author_facet | Brandon M. Stephens Paolo Stincone Daniel Petras Chance J. English Keri Opalk Stephen Giovannoni Craig A. Carlson |
| author_sort | Brandon M. Stephens |
| collection | DOAJ |
| description | Abstract Oxygen consumption by oceanic microbes can predict respiration (CO2 production) but requires an assumed respiratory quotient (RQ; ΔO2/ΔCO2). Measured apparent RQs (ARQs) can be impacted by various processes, including nitrification and changes in dissolved organic matter (DOM) composition, leading to discrepancies between ARQ and actual RQ. In DOM remineralization experiments conducted in the eastern North Atlantic Ocean, ARQs averaged 1.39 ± 0.14, similar to predictions for complete consumption of plankton biomass. DOM removed with an elevated nominal oxidation state (i.e., more oxidized DOM), as detected by liquid chromatography-tandem mass spectrometry, coincided with increased hydrolyzable amino acid removal, increased ARQs and bacterioplankton respiration (BR), and a decreased bacterioplankton growth efficiency (BGE). Across experiments, evidence emerged that nitrification and DOM partial oxidation, driven in part by bacterioplankton members of OM43, SAR92 and Rhodobacteraceae, can elevate BR relative to bacterioplankton consumption of plankton-derived carbon. These rare synoptic measurements of interrelated variables reveal complex biochemical and cellular processes underlying variability in large-scale CO2 production estimates. |
| format | Article |
| id | doaj-art-3df6e1ee891d4f5d9df966a1fe065865 |
| institution | Kabale University |
| issn | 2399-3642 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| series | Communications Biology |
| spelling | doaj-art-3df6e1ee891d4f5d9df966a1fe0658652025-02-02T12:37:20ZengNature PortfolioCommunications Biology2399-36422025-01-018111410.1038/s42003-025-07574-2Oxidation state of bioavailable dissolved organic matter influences bacterioplankton respiration and growth efficiencyBrandon M. Stephens0Paolo Stincone1Daniel Petras2Chance J. English3Keri Opalk4Stephen Giovannoni5Craig A. Carlson6Marine Science Institute/Department of Ecology, Evolution and Marine Biology, University of CaliforniaCMFI Cluster of Excellence, Interfaculty Institute of Microbiology and Medicine, University of TuebingenCMFI Cluster of Excellence, Interfaculty Institute of Microbiology and Medicine, University of TuebingenMarine Science Institute/Department of Ecology, Evolution and Marine Biology, University of CaliforniaMarine Science Institute/Department of Ecology, Evolution and Marine Biology, University of CaliforniaOregon State UniversityMarine Science Institute/Department of Ecology, Evolution and Marine Biology, University of CaliforniaAbstract Oxygen consumption by oceanic microbes can predict respiration (CO2 production) but requires an assumed respiratory quotient (RQ; ΔO2/ΔCO2). Measured apparent RQs (ARQs) can be impacted by various processes, including nitrification and changes in dissolved organic matter (DOM) composition, leading to discrepancies between ARQ and actual RQ. In DOM remineralization experiments conducted in the eastern North Atlantic Ocean, ARQs averaged 1.39 ± 0.14, similar to predictions for complete consumption of plankton biomass. DOM removed with an elevated nominal oxidation state (i.e., more oxidized DOM), as detected by liquid chromatography-tandem mass spectrometry, coincided with increased hydrolyzable amino acid removal, increased ARQs and bacterioplankton respiration (BR), and a decreased bacterioplankton growth efficiency (BGE). Across experiments, evidence emerged that nitrification and DOM partial oxidation, driven in part by bacterioplankton members of OM43, SAR92 and Rhodobacteraceae, can elevate BR relative to bacterioplankton consumption of plankton-derived carbon. These rare synoptic measurements of interrelated variables reveal complex biochemical and cellular processes underlying variability in large-scale CO2 production estimates.https://doi.org/10.1038/s42003-025-07574-2 |
| spellingShingle | Brandon M. Stephens Paolo Stincone Daniel Petras Chance J. English Keri Opalk Stephen Giovannoni Craig A. Carlson Oxidation state of bioavailable dissolved organic matter influences bacterioplankton respiration and growth efficiency Communications Biology |
| title | Oxidation state of bioavailable dissolved organic matter influences bacterioplankton respiration and growth efficiency |
| title_full | Oxidation state of bioavailable dissolved organic matter influences bacterioplankton respiration and growth efficiency |
| title_fullStr | Oxidation state of bioavailable dissolved organic matter influences bacterioplankton respiration and growth efficiency |
| title_full_unstemmed | Oxidation state of bioavailable dissolved organic matter influences bacterioplankton respiration and growth efficiency |
| title_short | Oxidation state of bioavailable dissolved organic matter influences bacterioplankton respiration and growth efficiency |
| title_sort | oxidation state of bioavailable dissolved organic matter influences bacterioplankton respiration and growth efficiency |
| url | https://doi.org/10.1038/s42003-025-07574-2 |
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