Blood biochemistry changes in a minipig infarction model
IntroductionThe present study aimed to assess changes in biochemical parameters during the adaptation of the myocardial infarction model to a conventional Hungarian minipig breed. According to our hypothesis, changes in the blood level of the necroenzymes are not only related to the interventional p...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2025.1493660/full |
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| author | Dénes Kőrösi Ágoston Göcző Noémi Varga Rita Garamvölgyi Nándor Balogh Kornélia Farkas András Vorobcsuk András Vorobcsuk |
| author_facet | Dénes Kőrösi Ágoston Göcző Noémi Varga Rita Garamvölgyi Nándor Balogh Kornélia Farkas András Vorobcsuk András Vorobcsuk |
| author_sort | Dénes Kőrösi |
| collection | DOAJ |
| description | IntroductionThe present study aimed to assess changes in biochemical parameters during the adaptation of the myocardial infarction model to a conventional Hungarian minipig breed. According to our hypothesis, changes in the blood level of the necroenzymes are not only related to the interventional procedure but are also influenced by peri-procedural animal keeping and treatment conditions.MethodsClosed chest acute myocardial infarction (AMI) was induced by balloon occlusion for 90 min in the left anterior descendent coronary artery (LAD) in 24 adult, female Pannon minipigs followed by reperfusion. Blood samples were taken before AMI, and immediately after the reperfusion, during the cardiac magnetic resonance imaging (cMRI) on days 3 and 30. Aspartate transaminase (AST), alanine aminotransferase (ALT), creatine kinase (CK), lactate dehydrogenase (LDH), and high-sensitivity troponin I were determined.ResultsWhile the parameters measured at baseline remained within physiological ranges, a notable elevation was seen in comparison with the results observed on day 30. This phenomenon was evident in all the laboratory parameters tested, except hs-troponin. The results for AST, ALT, LDH, and CK were statistically significant (p = 0.011, p = 0.001, p = 0.013, and p = 0.001, respectively). A statistically significant difference was observed between the baseline and 30-day AST/ALT ratio (p = 0.00514).DiscussionThe elevated levels of necroenzymes observed at baseline are likely to be a consequence of the physical and social stress imposed by the study design on the minipigs during the 72-h period prior to intervention. It is essential to define the optimal timing of baseline blood tests to ensure the reliability of the biochemical profile in a large animal infarction model. |
| format | Article |
| id | doaj-art-3ddab4a96c6a43d3acc6cdf4fe2d2f9a |
| institution | Kabale University |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-3ddab4a96c6a43d3acc6cdf4fe2d2f9a2025-01-27T06:40:35ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-01-011210.3389/fvets.2025.14936601493660Blood biochemistry changes in a minipig infarction modelDénes Kőrösi0Ágoston Göcző1Noémi Varga2Rita Garamvölgyi3Nándor Balogh4Kornélia Farkas5András Vorobcsuk6András Vorobcsuk7Doctoral School in Animal Science, Hungarian University of Agriculture and Life Sciences, Kaposvár, HungaryMedical School, University of Pécs, Pécs, HungaryCardio-Econom Ltd., Görcsöny, HungaryDoctoral School in Animal Science, Hungarian University of Agriculture and Life Sciences, Kaposvár, HungaryPraxislab Ltd., Budapest, HungaryMedical School, Institute of Bioanalysis, University of Pécs, Pécs, HungaryMedical School, University of Pécs, Pécs, HungaryDepartment of Cardiology, Kaposi Moritz Teaching Hospital, Kaposvár, HungaryIntroductionThe present study aimed to assess changes in biochemical parameters during the adaptation of the myocardial infarction model to a conventional Hungarian minipig breed. According to our hypothesis, changes in the blood level of the necroenzymes are not only related to the interventional procedure but are also influenced by peri-procedural animal keeping and treatment conditions.MethodsClosed chest acute myocardial infarction (AMI) was induced by balloon occlusion for 90 min in the left anterior descendent coronary artery (LAD) in 24 adult, female Pannon minipigs followed by reperfusion. Blood samples were taken before AMI, and immediately after the reperfusion, during the cardiac magnetic resonance imaging (cMRI) on days 3 and 30. Aspartate transaminase (AST), alanine aminotransferase (ALT), creatine kinase (CK), lactate dehydrogenase (LDH), and high-sensitivity troponin I were determined.ResultsWhile the parameters measured at baseline remained within physiological ranges, a notable elevation was seen in comparison with the results observed on day 30. This phenomenon was evident in all the laboratory parameters tested, except hs-troponin. The results for AST, ALT, LDH, and CK were statistically significant (p = 0.011, p = 0.001, p = 0.013, and p = 0.001, respectively). A statistically significant difference was observed between the baseline and 30-day AST/ALT ratio (p = 0.00514).DiscussionThe elevated levels of necroenzymes observed at baseline are likely to be a consequence of the physical and social stress imposed by the study design on the minipigs during the 72-h period prior to intervention. It is essential to define the optimal timing of baseline blood tests to ensure the reliability of the biochemical profile in a large animal infarction model.https://www.frontiersin.org/articles/10.3389/fvets.2025.1493660/fullblood biochemicalscardiac markersclosed chest infarctlarge animal modelminipigstress |
| spellingShingle | Dénes Kőrösi Ágoston Göcző Noémi Varga Rita Garamvölgyi Nándor Balogh Kornélia Farkas András Vorobcsuk András Vorobcsuk Blood biochemistry changes in a minipig infarction model Frontiers in Veterinary Science blood biochemicals cardiac markers closed chest infarct large animal model minipig stress |
| title | Blood biochemistry changes in a minipig infarction model |
| title_full | Blood biochemistry changes in a minipig infarction model |
| title_fullStr | Blood biochemistry changes in a minipig infarction model |
| title_full_unstemmed | Blood biochemistry changes in a minipig infarction model |
| title_short | Blood biochemistry changes in a minipig infarction model |
| title_sort | blood biochemistry changes in a minipig infarction model |
| topic | blood biochemicals cardiac markers closed chest infarct large animal model minipig stress |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2025.1493660/full |
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