Morphological and biochemical characterization of Ethiopian mustard (Brassica carinata A. Braun) germplasm grown in Central Ethiopia

Ethiopian mustard (Brassica carinata A. Braun) shows potential for diverse applications, including as leafy greens, green manure and oilseed feedstock for biofuels. This study evaluated the seed and oil production potential and phenotypic diversity of 49 B. carinata accessions through trials conduc...

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Main Authors: Ermias Estifanos, Tileye Feyissa, Kassahun Tesfaye, Alemu Lencho, Christina Eynck
Format: Article
Language:English
Published: Bioversity International 2025-05-01
Series:Genetic Resources
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Online Access:https://www.genresj.org/index.php/grj/article/view/243
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author Ermias Estifanos
Tileye Feyissa
Kassahun Tesfaye
Alemu Lencho
Christina Eynck
author_facet Ermias Estifanos
Tileye Feyissa
Kassahun Tesfaye
Alemu Lencho
Christina Eynck
author_sort Ermias Estifanos
collection DOAJ
description Ethiopian mustard (Brassica carinata A. Braun) shows potential for diverse applications, including as leafy greens, green manure and oilseed feedstock for biofuels. This study evaluated the seed and oil production potential and phenotypic diversity of 49 B. carinata accessions through trials conducted in 2018 at the Holeta and Asela Research Centers in Ethiopia, using a lattice design. Data were collected on phenological, morphological, agronomic and seed quality traits. The analysis revealed significant variability across most traits, except for silique width and oil and protein content at Asela, and main raceme length and total glucosinolate content at Holeta. Combined analysis showed significant genotype-by-location interactions for flowering date, seeds per silique and seed yield per hectare, indicating a strong environmental influence on these traits. Phenotypic and genotypic correlation analyses identified strong positive correlations between leaf traits and phenology, seed yield and seed quality, while oil content was negatively associated with protein and glucosinolate content. Principal component analysis identified five components at Asela and six components at Holeta with eigenvalues greater than one, explaining over 77% of the total variation at both locations. Key traits such as plant height, seed yield and oil content contributed significantly to these principal components. Cluster analysis grouped the accessions into three clusters based on distinct trait combinations. Accessions 17545, 21373, 24203 and 24495 consistently performed well across multiple traits across sites, making them strong candidates for breeding programmes focused on improving seed yield and quality in B. carinata.
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spelling doaj-art-3db6624e87c24dd6af89b79f6d2eea0a2025-08-20T01:51:54ZengBioversity InternationalGenetic Resources2708-37642025-05-0161110.46265/genresj.ZFEE2261Morphological and biochemical characterization of Ethiopian mustard (Brassica carinata A. Braun) germplasm grown in Central EthiopiaErmias Estifanos0Tileye Feyissa1Kassahun Tesfaye2Alemu Lencho3Christina Eynck4Department of Plant Science, Ambo University, Guder, EthiopiaInstitute of Biotechnology, Addis Ababa University, Addis Ababa, EthiopiaBio and Emerging Technology Institute, Addis Ababa, EthiopiaDepartment of Plant Science, Ambo University, Guder, EthiopiaSaskatoon Research and Development Centre, Agriculture and Agri-Food Canada, Saskatoon, SK, Canada Ethiopian mustard (Brassica carinata A. Braun) shows potential for diverse applications, including as leafy greens, green manure and oilseed feedstock for biofuels. This study evaluated the seed and oil production potential and phenotypic diversity of 49 B. carinata accessions through trials conducted in 2018 at the Holeta and Asela Research Centers in Ethiopia, using a lattice design. Data were collected on phenological, morphological, agronomic and seed quality traits. The analysis revealed significant variability across most traits, except for silique width and oil and protein content at Asela, and main raceme length and total glucosinolate content at Holeta. Combined analysis showed significant genotype-by-location interactions for flowering date, seeds per silique and seed yield per hectare, indicating a strong environmental influence on these traits. Phenotypic and genotypic correlation analyses identified strong positive correlations between leaf traits and phenology, seed yield and seed quality, while oil content was negatively associated with protein and glucosinolate content. Principal component analysis identified five components at Asela and six components at Holeta with eigenvalues greater than one, explaining over 77% of the total variation at both locations. Key traits such as plant height, seed yield and oil content contributed significantly to these principal components. Cluster analysis grouped the accessions into three clusters based on distinct trait combinations. Accessions 17545, 21373, 24203 and 24495 consistently performed well across multiple traits across sites, making them strong candidates for breeding programmes focused on improving seed yield and quality in B. carinata. https://www.genresj.org/index.php/grj/article/view/243Brassica carinataGermplasm characterizationoil contentseed yield
spellingShingle Ermias Estifanos
Tileye Feyissa
Kassahun Tesfaye
Alemu Lencho
Christina Eynck
Morphological and biochemical characterization of Ethiopian mustard (Brassica carinata A. Braun) germplasm grown in Central Ethiopia
Genetic Resources
Brassica carinata
Germplasm characterization
oil content
seed yield
title Morphological and biochemical characterization of Ethiopian mustard (Brassica carinata A. Braun) germplasm grown in Central Ethiopia
title_full Morphological and biochemical characterization of Ethiopian mustard (Brassica carinata A. Braun) germplasm grown in Central Ethiopia
title_fullStr Morphological and biochemical characterization of Ethiopian mustard (Brassica carinata A. Braun) germplasm grown in Central Ethiopia
title_full_unstemmed Morphological and biochemical characterization of Ethiopian mustard (Brassica carinata A. Braun) germplasm grown in Central Ethiopia
title_short Morphological and biochemical characterization of Ethiopian mustard (Brassica carinata A. Braun) germplasm grown in Central Ethiopia
title_sort morphological and biochemical characterization of ethiopian mustard brassica carinata a braun germplasm grown in central ethiopia
topic Brassica carinata
Germplasm characterization
oil content
seed yield
url https://www.genresj.org/index.php/grj/article/view/243
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