Identification and Characterization of Wheat Streak Mosaic Virus Isolates in Wheat-Growing Areas in Brazil
Wheat streak mosaic virus (WSMV—Potyviridae, Tritimovirus), transmitted by the eriophyid mite Aceria tosichella Keifer (Acari: Eriophyidae), was considered a quarantine pest in South America. Since the first report of virus in Argentina, the vector has been found in Argentina, Uruguay and Brazil. Th...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2013-01-01
|
| Series: | International Journal of Agronomy |
| Online Access: | http://dx.doi.org/10.1155/2013/983414 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832567027469910016 |
|---|---|
| author | Talita Bernardon Mar Douglas Lau Jurema Schons Paulo Roberto Valle da Silva Pereira Alan Johnny Carminatti |
| author_facet | Talita Bernardon Mar Douglas Lau Jurema Schons Paulo Roberto Valle da Silva Pereira Alan Johnny Carminatti |
| author_sort | Talita Bernardon Mar |
| collection | DOAJ |
| description | Wheat streak mosaic virus (WSMV—Potyviridae, Tritimovirus), transmitted by the eriophyid mite Aceria tosichella Keifer (Acari: Eriophyidae), was considered a quarantine pest in South America. Since the first report of virus in Argentina, the vector has been found in Argentina, Uruguay and Brazil. The objective of this work was to determine the occurrence of WSMV in Brazil and characterize isolates from wheat-growing areas. Between 2009 and 2011, a total of 40 samples collected in wheat (Triticum aestivum) growing areas, where the presence of the mite was previously related, were tested by RT-PCR for virus detection. Six isolates of WSMV were obtained and characterized by sequencing. Two of them had their host range determined. The Brazilian WSMV isolates clustered in clade D are closely related to the Argentine isolate Arg2 (FJ348359). As expected, isolates were unable to infect dicotyledons plants. They caused mosaic in Triticum aestivum, Hordeum vulgare, Secale cereale, ×Triticosecale, and Zea mays, and they were also able to infect Avena sativa. The presence of WSMV in wheat-growing areas of the Brazilian state Rio Grande do Sul was confirmed, possibly having been introduced from a common source and/or direct vector bearing virus migration from Argentina. |
| format | Article |
| id | doaj-art-3d5b372a8bd64bd5836d1bb9d93a8b2d |
| institution | Kabale University |
| issn | 1687-8159 1687-8167 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Agronomy |
| spelling | doaj-art-3d5b372a8bd64bd5836d1bb9d93a8b2d2025-02-03T01:02:28ZengWileyInternational Journal of Agronomy1687-81591687-81672013-01-01201310.1155/2013/983414983414Identification and Characterization of Wheat Streak Mosaic Virus Isolates in Wheat-Growing Areas in BrazilTalita Bernardon Mar0Douglas Lau1Jurema Schons2Paulo Roberto Valle da Silva Pereira3Alan Johnny Carminatti4Universidade de Passo Fundo, BR 285, Km 292, 99001-970 Passo Fundo, RS, BrazilEmbrapa Trigo, BR 285, Km 294, 99001-970 Passo Fundo, RS, BrazilUniversidade de Passo Fundo, BR 285, Km 292, 99001-970 Passo Fundo, RS, BrazilEmbrapa Trigo, BR 285, Km 294, 99001-970 Passo Fundo, RS, BrazilUniversidade de Passo Fundo, BR 285, Km 292, 99001-970 Passo Fundo, RS, BrazilWheat streak mosaic virus (WSMV—Potyviridae, Tritimovirus), transmitted by the eriophyid mite Aceria tosichella Keifer (Acari: Eriophyidae), was considered a quarantine pest in South America. Since the first report of virus in Argentina, the vector has been found in Argentina, Uruguay and Brazil. The objective of this work was to determine the occurrence of WSMV in Brazil and characterize isolates from wheat-growing areas. Between 2009 and 2011, a total of 40 samples collected in wheat (Triticum aestivum) growing areas, where the presence of the mite was previously related, were tested by RT-PCR for virus detection. Six isolates of WSMV were obtained and characterized by sequencing. Two of them had their host range determined. The Brazilian WSMV isolates clustered in clade D are closely related to the Argentine isolate Arg2 (FJ348359). As expected, isolates were unable to infect dicotyledons plants. They caused mosaic in Triticum aestivum, Hordeum vulgare, Secale cereale, ×Triticosecale, and Zea mays, and they were also able to infect Avena sativa. The presence of WSMV in wheat-growing areas of the Brazilian state Rio Grande do Sul was confirmed, possibly having been introduced from a common source and/or direct vector bearing virus migration from Argentina.http://dx.doi.org/10.1155/2013/983414 |
| spellingShingle | Talita Bernardon Mar Douglas Lau Jurema Schons Paulo Roberto Valle da Silva Pereira Alan Johnny Carminatti Identification and Characterization of Wheat Streak Mosaic Virus Isolates in Wheat-Growing Areas in Brazil International Journal of Agronomy |
| title | Identification and Characterization of Wheat Streak Mosaic Virus Isolates in Wheat-Growing Areas in Brazil |
| title_full | Identification and Characterization of Wheat Streak Mosaic Virus Isolates in Wheat-Growing Areas in Brazil |
| title_fullStr | Identification and Characterization of Wheat Streak Mosaic Virus Isolates in Wheat-Growing Areas in Brazil |
| title_full_unstemmed | Identification and Characterization of Wheat Streak Mosaic Virus Isolates in Wheat-Growing Areas in Brazil |
| title_short | Identification and Characterization of Wheat Streak Mosaic Virus Isolates in Wheat-Growing Areas in Brazil |
| title_sort | identification and characterization of wheat streak mosaic virus isolates in wheat growing areas in brazil |
| url | http://dx.doi.org/10.1155/2013/983414 |
| work_keys_str_mv | AT talitabernardonmar identificationandcharacterizationofwheatstreakmosaicvirusisolatesinwheatgrowingareasinbrazil AT douglaslau identificationandcharacterizationofwheatstreakmosaicvirusisolatesinwheatgrowingareasinbrazil AT juremaschons identificationandcharacterizationofwheatstreakmosaicvirusisolatesinwheatgrowingareasinbrazil AT paulorobertovalledasilvapereira identificationandcharacterizationofwheatstreakmosaicvirusisolatesinwheatgrowingareasinbrazil AT alanjohnnycarminatti identificationandcharacterizationofwheatstreakmosaicvirusisolatesinwheatgrowingareasinbrazil |