Preparation and application of porcine broadly neutralizing monoclonal antibodies in an immunoassay for efficiently detecting neutralizing antibodies against foot-and-mouth disease virus serotype O

Preparation and application of porcine broadly neutralizing monoclonal antibodies in an immunoassay for efficiently detecting neutralizing antibodies against foot-and-mouth disease virus serotype O

ABSTRACT Neutralizing antibodies provide vital protection against foot-and-mouth disease virus (FMDV). The virus neutralization test (VNT) is a gold standard method for the detection of neutralizing antibodies. However, its application is limited due to the requirement for live virus and unsuitabili...

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Main Authors: Yimei Cao, Fengjuan Li, Xiangchuan Xing, Huiyan Zhang, QiongQiong Zhao, Pu Sun, Yuanfang Fu, Pinghua Li, Xueqing Ma, Jing Zhang, Zhixun Zhao, Hong yuan, Jian Wang, Tao Wang, Huifang Bao, Xingwen Bai, Dong Li, Qiang Zhang, Kun Li, Zengjun Lu
Format: Article
Language:English
Published: American Society for Microbiology 2025-02-01
Series:Microbiology Spectrum
Subjects:
foot-and-mouth disease virus
porcine broadly neutralizing monoclonal antibody
competitive ELISA
neutralizing antibody
serotype O
Online Access:https://journals.asm.org/doi/10.1128/spectrum.02234-24
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